1977-1980
HISTOLOGICAL INVESTIGATIONS ON KIDNEY OF SILDENAFIL CITRATE (EDEGRA) TREATED ALBINO MICE
Authors: K. V. P. Suriyakumari *1, R. Udayakumar 2, T. Ruba 2.
Number of views: 464
Background: Edegra (Sildenafil citrate), an effective oral therapy for Erectile Dysfunction, being the citrate salt of Sildenafil is a selective inhibitor of cGMP- specific phosphodiesterase type 5 (PDE 5).
Aim: To study the long- term impact of Sildenafil citrate on the histological components of kidney of Albino mice.
Materials and Methods: Thirty six healthy male Wistar Albino mice were selected on weight basis and divided into six groups (S1, S2, S3, S4, S5 and S6), each consisting of six animals in it. Here, Group S1 and S5 served as the control while the rest of the four groups (i.e. S2, S3, S4 and S6) served as the experimental ones. Group S1 animals were treated with a single dosage of conductivity water while the experimental animals, namely, S2, S3 and S4 were treated with a single dosage of the chosen drug (@ 1µg/g body wt.) and sacrificed after 1 hr., 4 hrs. and 24 hrs. of the last dosage. Group S5 animals were treated daily with a single dosage of conductivity water while the group S6 animals were treated daily with a single dosage of the chosen drug (@ 1µg/g body wt.) for all the 15 days and then sacrificed after 4 hrs. of the last dosage. Vertical ventral midline incision was made in the abdominal wall to collect both the kidney samples. The organs were preserved in 10% formalin saline, processed and stained with Eosin and Haematoxylin stains.
Results: Histopathological observations have been made on the collected kidney samples of Albino mice treated with Sildenafil citrate (Edegra). Increase in the periglomerular spaces, shrinkage of Glomeruli, increased number of vacuolated cells and reduced number of Glomeruli in cortical area due to destruction of Glomeruli have been strikingly noticed for long- term drug treated samples.
Conclusion: Sildenafil citrate (Edegra), if administered for a long- term, will produce drastic impacts on the histoarchitecture and vital functions of kidney of Albino mice.
KEY WORDS: Albino mice, Kidney, Edegra, Histology, Vacuolated cells.