146-151
Laboratory Scale Comparative Studies of Penicillin Antibiotic Production from Various Carbon Sources
Authors: Ngerebara NN, Amadi LO
Number of views: 231
Comparative penicillin antibiotic yields or production from various carbon sources was studied in the laboratory. Surface culture method for assessment of growth of the fungus, Pencillium notatum and penicillin yields was employed. The basic nutrient medium; corn steep liquor, standard salts with initial pH of 4.0 were used throughout the study. The basic medium was supplemented with 3 percent (3%) and 6 percent (6%) of the various carbon sources; glucose, lactose, cornstarch, glycerol, brown sugar, sorbitol and corn dextrin respectively. Penicillin yields for 7-days period at 3% concentration of glucose, brown sugar, glycerol, cornstarch, corn dextrin, and lactose were 66mg/ml, 79 mg/ml, 52mg/ml, 146mg/ml, 146mg/ml and 146mg/ml respectively. Similarly, at 6% concentration penicillin yields were 50mg/ml, 45mg/ml, 40mg/ml, 42mg/ml and
105mg/ml respectively. At 3% concentration, lactose, cornstarch, corn dextrin and brown sugar were more beneficial for penicillin production, glycerol being definitely inferior. Increases in pH of the broth were less rapid in the lactose, starch and dextrin cultures compared to those of glucose, sucrose and glycerol cultures. But at 6% concentration, there was less fluctuation in pH in the cultures containing lactose than those of glucose, sucrose, glycerol or sorbitol respectively. Under these conditions, there was no corresponding increase in penicillin yields as the concentration was increased from 3% to 6%. However, at both 3% and 6% concentrations, cultures containing lactose gave the highest penicillin yields and therefore should be the substrate of choice even in industrial application.
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