International Journal of Ayurveda and Pharmaceutical Chemistry

12

Authors: Dammalapati Srikantha1 and Rudra Raju Ramesh Raju1*

A reversed phase-high performance liquid chromatography (RP-HPLC) method was developed for determination of Lorazepam in the tablet dosage form. The developed method was validated by measuring the parameters such as linearity range, precision, limit of detection (LOD), robustness, ruggedness, drug recovery and for the system suitability. In the HPLC method, acetonitrile: methanol (65:35 v/v) was the mobile phase, C18 column (250 mm x 4.5 mm x 5 μm), pH 4.3 and detection wavelength was 224 nm. The measured retention time of Lorazepam was found to be 4.68 minutes, which is the shortest time compared to the values reported so far. The limit of detection was 0.3μg/ml and the linearity was found to be in the range 10-100 μg/ml. The correlation coefficient value was 0.9998 and a low relative standard deviation (RSD < 1%) was obtained for linearity, precision and robustness. From the above observed parameters it can be concluded that the developed method satisfies to be a powerful tool in determination of Lorazepam drug in tablet dosage forms.

11

Authors: G.Y.S.K. Swamy1 *and P. Sivanarayanan

Medicinal plants contain essential and trace elements, which are very important for the human body. India had a rich experience of health benefits through utilising many varieties of Ayurvedic medicinal herbs. Our emphasis in the current paper is on the characterization of five Medicinal plants (Oroxylum indicum (Linn.) Vent., Leucas indica (L.) R. Br. Ex Vatke, Premna tomentosa (Willd.), Piper chaba (Hunter) and Hedychium spicatum (Buch.-Ham.) for their macro and microelement contents using wavelength dispersive X-ray fluorescence (WD-XRF) spectrometry. The applicability of the method particularly for the simultaneous determination of sixteen elements (Na, Mg, Al, Si, P, S, Cl, K, Ca, Cr, Mn, Fe, Cu, Zn, Ba and Pb) in biological matrices has been evaluated in terms of the detection limit, precision and accuracy. The method was validated by analyzing five Chinese certified reference materials (NCS ZC73012 (cabbage), NCS ZC73013 (spinach), NCS ZC73017 (apple), NCS ZC85006 (tomato) and NCS DC73348 (bush, branches and leaves)) of vegetable standards. In general, good agreement was achieved between certified values and measured ones with recoveries ranging from 92 to 108%. The WD-XRF method proposed proved to be an effective tool for the investigation and quality control processes of vegetation samples.

10

Authors: Kanchan M Borkar1*, Anantkumar V Shekokar2 and Sandhya A Kolekar3

In modern aspect recurrence of venous ulcer, after conservative treatment, is very high. Direct venous surgery is difficult due to absence of suitable prosthetic graft and difficulty in reconstructing venous valve. The healing of wound still remains a major problem to the surgeon as well as to the patient. In Ayurveda the primary aim of the treatment is to give an effective and safe approach to this perplexing problem. Shonit Sanghat Bhedana, Marg Vivruanan, Bandh Chhinnana, Twak Mansa Sthirikaran, Lekhana, Lasikapuy and Shleshma Upshoshan are the Karmas (propertities)of Katu tikta Rasa . Therefore, we have selected Katu andTikta Raspradhana Kalpa which are Navjivan Rasa & Ropana Tailm for Antaparimarjan & Bahiparimarjan respectively medicaments of venous ulcer . In using Ayurvedic medicinal remedy we avoid surgery and its complication with saving of time and money. In the following article an attempt will be made for proper understanding of the historical aspect of the Dushta Vrana (Infected wound), its etiopathology, methodology of ulcer healing, in the light of Ayurvedic and modern knowledge. The clinical study was conducted on two patients who were selected randomly and divided in two groups. First experimental group treated with Navjivan Rasa and Ropana Tailm and second control group treated with Diosmin 300mg BID. The clinical assessment was done on the basis of grading criteria with basic symptomatology pain, local temperature, discharge, color and smell. Navjivan Rasa and Ropan tailm possess sufficient efficacy in Vran shodhana and Vran ropana and shows better results in healing of venous ulcer.

11

Authors: Sriram Chandra Mishra*

Gridhrasi (Sciatica) is a major health problem related to locomotor system in both developed and developing countries. There is close association between low back pain and Sciatica. The prevalence data on low back pain and Sciatica shows the problematic status of the disease. As many as 90% of patients with acute back pain return to work within three months, but many experience symptoms of recurrence and function limitations. This hospital based observational piloting study on Godugdha yukta Vaitaran basti evaluates its therapeutic efficacy on Vataja Gridhrasi. For control study, Katibasti with sahacharadi tailam is taken which is a well known & proven procedure for lumbosacral disorders. The study was conducted on sixty clinically diagnosed cases of Vataja Gridhrasi (Sciatica) on the basis of various subjective & objective scientific parameters. Clinically and statistically improvements were observed after completion of therapy which was more in Vaitaran basti group rather than Katibasti group. Both drugs were well tolerated and patients were not forced to discontinue drug treatment out of drug intolerance. However in the series of trial 100 % recovery leading to Cure could not be achieved. This may be due to the reason that Basti chikitsa is said to be Ardhachikitsa. Godugdha yukta Vaitaran basti has been found to be an effective therapeutic regimen in the management of Vataja Gridhrasi.

7

Authors: Padma Harshan 1*, Geeta Sukumaran 2and Uttam Nagwanshi 3

Maranta arundinacea (Arrowroot or West Indian Arrowroot) is indigenous to the West Indies, where native people, use the powder. The Arawaks used the substance to draw out toxins from people wounded by poisoned arrows. Its name is thought to be derived from that practice. The name may also come from the native Caribbean Arawak people's aru-aru (meal of meals), for which the plant is a staple. Arrowroot has been used as an infant formula in place of breast milk or to help the baby adjust after weaning believed to be the easiest to digest. Because of its demulcent properties it has been used for various bowel complaints. Also, it is believed to be useful against poisoned wounds, including scorpion stings, snake bites, and spider bites and gangrene. Consuming fresh arrowroot juice mixed with water is said to be an antidote to vegetable poisons. Arrowroot is used as a herbal remedy to alleviate nausea and to replenish nutrients lost through diarrhea and vomiting. It’s a nutritious diet for people with certain chronic diseases, during recovery from an illness or for certain internal irritations including bladder irritation. The results of non-chemically grown arrowroots have been encouraging with a good production in less than a quarter acre of land that depicts a high economic viability with such practices. It can prove to be a remunerative medicinal plant with semi-processing to small and marginal farmers. Another very important feature observed was that the seeds were not treated with any fungicide or chemicals during preservation thus avoiding use of chemicals and keeping it healthy.