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Comparative Investigation on Fourier Transform Infrared Spectra of Unmodified, Extract and Modified Cola Lepidota seed
Authors: Millicent U. Ibezim-Ezeani, Armstrong E. Pat-Okunbor, Chidi Obi
Number of views: 260
This study examined the spectra of the seed of cola lepidota in the unmodified form, the acetone extract and the acetone extract modified with oxalic acid. The comparison of the spectra data obtained using Fourier Transform Infrared (FTIR) was carried out to resolve the functional groups present in cola lepidota seed. The seeds of cola lepidota were air dried, pulverized to particle size of 150µm and stored at 28oC. The acetone extract was obtained using soxhlet extraction method while the acetone extract was reacted with oxalic acid in the modification process. The unmodified, acetone extract and the oxalic acid-modified extract were analyzed using FTIR in the wave number range of 650-4000 cm-1. Result analyses revealed the likely presence of =C-H, C-O, C-O-C, O=C-O-O, C=C, C=O and O-H functional groups. The characteristic peaks at 748.41 and 840.99 cm-1 for the spectrum of unmodified cola lepidota seed reflects bond deformation, while that at 3279.10 and 3387.11 cm-1 for the unmodified and extract respectively which disappeared in oxalic acid-modified cola lepidota seed corresponds to the formation of new compound. The formation of oxalic acid-modified cola lepidota seed extract was indicated by the variation of percentage transmittance with wave number (103.0-111.5 %, 86.0-111.6 % and 86.0-109.8 % for the unmodified, acetone extract and extract modified with oxalic acid respectively), as well as differences in absorption band intensities in relation to functional groups per molecule.