Journal of World's Poultry Research

32-37

Authors: Boussouar H, Khenenou T, Bennoune O and Berghiche A.

Excessive alcohol consumption by a pregnant woman may delay foetal development and may cause malformations. In this study, the model of the chicken embryo to demonstrate the teratogenic effect of ethanol (33%) on the chicken osteogenesis on the 10th day of embryonic development have been used. 49 fertilized eggs were used in present investigation. Hence, different doses of ethanol were injected into the chicken embryos at 33% (20, 40, 80μl) in the air space at gastrulation and, on the other hand, an equivalent amount of the mentioned doses of distilled water were injected into the control-group eggs which was done once in every two days in order to maintain a high concentration in the blood. Experiments were repeatedly and independently carried out for three times. The eggs were incubated in a humid incubator at the temperature of 37.7 °C and at 60-65% of humidity. On the 10th day of incubation, the embryos were taken out and fixed in formalin at 10%. After that, the eggs were sectioned at 5μm of thickness with a Leica micrtome and, then, stained with the Hematoxylin and eosin. Histological examination has revealed that the exposition of chicken embryos to ethanol (33%) delays the skeletal development in a dose-dependent manner by reducing the length of the cartilaginous proliferation zone and hypertrophic zone during the bone formation period. Furthermore, under the effect of ethanol, the cell proliferation activities were repressed. In conclusion, present results indicated that using ethanol to treat chicken embryos at early stages caused considerable malformations and a decreased in the embryo survival rate. The exposition to alcohol affects the chicken osteogenesis in a dose-dependent manner.

50-58

Authors: James G, Garba DJ, Adeolu AS, Adamu Z and Mamma Z.

The aim of the present study was to determine the effect of different bedding materials on the, hematological and serum biochemical parameters of the broiler chickens. A completely randomized design was employed for the research in which the treatment were five bedding materials (rice hulls, groundnut hulls, wood shaving, sharp sand and control). The treatments were replicated three times each with ten birds in each experimental unit. The birds were brooded for two weeks before the experiment begin. The blood samples were collected at day 56 of experiment for hematological and serum biochemical analysis. There was no significant difference in the glucose, serum total protein, globulin, calcium, sodium, total bilirubin, conjugated bilirubin, and serum alanine transferase, but there was a significant difference in serum cholesterol, albumin, bicarbonate and serum aspartate transferase within the groups. The findings also indicated in the hematological parameters that bedding materials caused significant difference in the hemoglobin content, white blood cell count and mean corpuscular hemoglobin, but no significant differences in the other parameters (Heterophils, monocytes, basophils, lymphocytes, mean cell volume and packed cell volume) were seen. The results suggested that rice, hulls, groundnut hulls and sharp sand can serve appropriately as bedding materials for broiler production, with compatible effects on serum biochemical and hematological parameters.

68-77

Authors: Kurniasih and Prakoso YA.

Aflatoxin is a worldwide problem in poultry industries as it is known to contaminate poultry feed. Aflatoxin induces stress and increases mortality rate during infection in poultry, especially broiler chickens. The objectives of this study was to observe the pathological effects due to aflatoxicosis in broiler chickens. A total of 120 chickens were divided into four groups, group A fed with a basal diet without aflatoxin contamination, group B with aflatoxin (> 1 ppb 51 ppb 101 ppb < 150 ppb). The data were collected and analysed on day 7, 14, and 21. The results showed that diet with high aflatoxin contamination in group D impaired the physical and laboratory performances of the chickens, increased the risk of residue in the poultry’s final products. The varying doses of aflatoxin contamination in the chicken feed causes the problems on the broiler chickens with different level of severity.

82-88

Authors: Habibi H, Ghahtan N and kohanmoo MA.

The current study was conducted to examine the influences of Sargassam cristafolium, Gracilaria pulvinata, Rhus coriaria, and Punica granatum peel dried powder in two levels (10 and 20gr/kg) of dietary on productive performance and some egg quality characteristics of laying Japanese quail. A total of 675 (49 days old) Japanese quail were randomly distributed into nine groups with three replicates of 25 birds in each. Results showed that egg weight, shell weight, albumen weight and shell thickness were not influenced by treatments. The effects of medicinal plants on weekly egg production differ depending on the dietary medicinal plant type and dosage. Moreover, the birds fed diet supplemented with Sargassum cristaefolium at 10g/kg had greater weekly egg production compared to others. Haugh unit was reduced by diet supplemented with R. coriaria. Diet supplemented with S. cristaefolium at 20 g/kg increased yolk weight. Furthermore, greater albumen protein and thiobarbituric acid content of the tenth day’s eggs (TBAd10) were perceived in birds fed diet supplemented with G. pulvinata. In present study, the lowest levels of cholesterol (9.66 mg/g) was determined in the egg yolk of groups R. coriaria and G. pulvinata, and the control group showed to have the most amounts (12.00 mg/g). Based on obtained data, addition of 0.5% and 2% of S. cristafolium, G. pulvinata, R. coriaria, and P. granatum peel to the diet lead to be progress in egg production rate, enhanced the egg quality and egg biochemical properties in Japanese quail.

98-108

Authors: Abdo SM, Amer SA, Ahmed HM, Mahmoud RH, Salama AA and Kutkat MA-A.

Using microalgal biomass in animal diets has been studied recently. Many species of cultivated algae were found effective in maintaining animal growth performance, and in improving body weight. Using of microalgae collected from high rate algal ponds (HRAP) as a feed additive to broilers ration was studied. One hundred and twenty broiler chicks were divided into 6 groups of 20 birds, three of them have fed on balanced broiler ration supplied with 1% weight per weight (W/W) of microalgae biomass and have variable vaccination schemes of live attenuated and inactivated Newcastle disease virus (NDV) vaccines genotype II or either non-vaccinated control. In addition, the other 3 groups have fed on free microalgae biomass balanced ration with the same vaccination treatment. Furthermore, weight gain, antibody response, mortalities, viral shedding and normal viability of chickens were estimated in order to assess the efficiency of microalgae as a feed additive. The results showed that the microalgae have no hazard effect on feed and water intake as well as enhanced viability of chickens. And in regards to immune function and body weight, they have similar effect with the free microalgae groups in normal serological response and viral shedding post vaccination with NDV vaccines as well as similar protection rate and body weight gain. In conclusion, microalgae can be used in broiler ration with no deleterious effect on growth rate, weight gain, poultry viability and immune response. In conclusion dried microalgal biomass harvested from HRAP can be used in broiler ration with no deleterious effect on growth rate, weight gain, poultry viability and immune response. Furthermore, future studies should be applied with increasing microalgae percent in poultry feed up to 5, 10 or 20% (W/W) in order to assess better performance on poultry production.

01-06

Authors: Sugiharto S.

In order to reduce the production cost, cassava pulp has been incorporated in chicken diets as an energy source. However, the use of such agro-industrial by-product may be confined by its higher fibre and lower protein contents. Improving the nutritional characteristics (lowering fibre and increasing protein content) through fungal solid state fermentation may be conducted to increase the inclusion level of cassava pulp in chicken rations. Apart from an energy source, fungal fermented cassava pulp (FCP) may also exert a beneficial effect on intestinal health of chickens, although further studies are needed to explore the functional benefit of FCP on chicken health.

15-23

Authors: Sarwar Inam AKM, Suzauddula Md and Kearney J.

Poultry meat is very popular in Ireland because of low cholesterol level. Ireland is in the top position for the consumption of poultry meat in whole Europe. Ireland emits 3.3 kg CO2- equivalent per kg of poultry for the poultry meat production which is the lowest amount among all the other European countries. To expand this sector with respect to environmental concern some issues need to be considered very carefully such as effective poultry feed production system, energy consumption in both poultry production and processing area, manure management system, wastewater and odour management systems. If these issues are not handled carefully, several types of harmful effect will occur in both living and environment cycle such as water borne diseases, global warming and ozone layer depletion. The objective of this report is to give an overview of the current situation of poultry production in Ireland, policies and legislation related to poultry production and to show the way to expand this sector in Ireland in line with current ecological concern.

81-94

Authors: Arya Kh, Gupta R and Laxmi Saxena V.

This Review aims to understand the present status of influenza viruses and its epidemiology. The first case in India has been reported in the Dasarahalli village near Bangalore after six months of India’s declaration that it is free from H5N1 and H5N8 from world organization for animal health. The recent controversy regarding outbreaks and cross-species barrier resulted in highly contagious infection with fatal outcomes, triggered menace all over India with remarkable economic consequences. Thus, we had reviewed epidemiology, virology, surveillance, transmission, detection, treatment and associated control measures to depict the current perspective of Influenza epidemic. We also studied different Quails and its comprehensive portal susceptible to influenza and in-depth genetic characterization of virus due to new viral mutant causing host-virus complications, virus mutation, and vaccination with its prompt administration as it is the urgency of the era. Addressing aspects of the epidemiology of the H5N1 and drug resistance genomic signatures infecting poultry and Humans helps to frontier our ability to minimize data gaps and maximize the better results of the available H5N1 studies.

100-104

Authors: Kumar R, Brar RS, Banga HS and Sodhi S.

Vitamin D3 is ten times more biologically active than vitamin D2, over supplementation of vitamin D3 causes hypercalcemia with deposition of calcium and phosphate as crystals in the visceral organs. Birds are considered more resistant to endotoxin and information on inflammation and homeostasis in birds supplemented with higher dose of vitamin D3 when suffer endotoxic shock is lacking. The present study was conducted to compare the effect on hemoglobin concentration and biochemical parameters of broiler chicks by administering toxic dose of vitamin D3 for 21 days concomitantly challenged with endotoxin. The chicks were randomly divided into four groups viz. A, B, C and D. Hemoglobin concentrations of control groups (A and B) and treatment groups (C and D) did not differ significantly (P< 0.05). Hypercalcemia and hyperphosphatemia was observed in both treatment groups in comparison to the control group. No significant (P< 0.05) change was observed in the concentrations of total protein and albumin and in the activity of plasma Alanine Aminotransferase, Aspartate Aminotransferase and Alkaline Phosphatase on day 28 of control (A and B) and treatment (C and D) groups. Vitamin D3 supplementation causes immunomodulation; hence acute endotoxic shock does not incite inflammatory response and disturb the homeostasis in broiler chicks.

111-119

Authors: Rasol Al-Kafajy F, Sahib Al-Shuhaib MB, Salah Al-Jashami Gh, Mohammed Al-Thuwaini T.

The study was conducted to compare body weight, egg, and carcass characteristics, as well as several biochemical parameters amongst three lines of plumage color of quails, including, black, white, and brown (n= 200 each). Body weight was analyzed on a weekly basis throughout the study period (third– 13th week of age). Eggs were collected for seven consecutive weeks of sexual maturity (seventh – 13th week of age). In addition to egg quality measurements, 16 serum biochemical parameters were also determined. The brown line had exerted significantly higher values of body weight in most analyzed weeks of sexual maturity. It had given higher values of albumen height and shell thickness, as well as carcass dressing than other lines. Simultaneously, a significantly high number of eggs in the white line were observed in the most analyzed weeks. Besides, it had given higher values in terms of shell and yolk weights, as well as several carcass characteristics, such as the heart, thigh, breast, and back. The biochemical analyses had shown no significant differences amongst the analyzed populations with exception of a higher concentration of amylase in the brown line. In conclusion, our study revealed the presence of a clear superiority of the brown and white lines in terms of the meat and egg productivity, respectively. Therefore, we recommend breeders to raise brown and white lines for a better production of meat and eggs, respectively, whereas the black line has shown the least productive characteristics than other two lines throughout the study period.

127-133

Authors: Sedeik M El-S, Elshal NA, Awad AM and Kandil N.

Avian influenza (AI) disease still threat poultry industry in Egypt causing great economic losses. In order to identify and characterize the agent of suggestive clinical cases of AI disease, 28 flocks showing clinical signs suspected to be due to AI infections have been investigated. By slide Haemagglutination (HA), the positive samples were 14/28 and concerning the results of real time- reverse transcriptase polymerase chain reaction (RRT-PCR), 2/14 samples were positive to AI H5, 7/14 to New castle disease virus (NDV), 1/14 to H9 and 4/14 co-infected (2 samples had NDV + AI H5 and others had NDV + AI H9). These positive PCR samples were subjected to further characterization by genotyping and sequencing analysis. The two isolated of H5 AI strain were classified to H5N8 which, related to Russian strains (clade 2.3.4.4) and the genetic analysis approved little relationship between these two H5N8 strain and the commercial AI vaccines with percent (80- 91.7%). So, the researchers should have more monitoring for these viral diseases with effective biosecurity and quarantine measures to minimize the disease occurrence.

50-58

Authors: Sayed RH, Ahmed HA, Shasha FA and Ali AM.

Mycoplasma gallisepticum is an economically important pathogen of poultry worldwide, causing chronic respiratory disease in chickens and turkeys. Vaccination of poultry with Mycoplasma gallisepticum live vaccines is an approach to reduce susceptibility to infection and to prevent economic losses. The goal of this study was to develop an alternative method for evaluation of live and killed vaccine using quantitative differential real time PCR (rt-PCR) assay. Real time PCR assay was implemented for titration and identification of three types of Mycoplasma gallisepticum (F, ts-11 and field strain). Three groups of chicks were vaccinated by using F- strain, ts-11 and killed vaccine and the forth group was considered control. Challenge test was appliedby using Mycoplasma gallisepticum field strain (108 CFU) at three weeks post vaccination. Antibody ELISA titers against Mycoplasma gallisepticum were 319, 259 and 1009 for F, t-11 and killed vaccine respectively at 3 weeks post vaccination. The protection rates were 81.5%, 74%, and 66.6% for F- strain, ts-11 and killed vaccine respectively that was determined by air sac lesion scour. Using quantitative differential rt-PCR for necropsied birds at 5 days post challenge 7days post challenge and 14 days post challenge demonstrated that the F-strain vaccine had ability to prevent shedding of field strain at 14 days post challenge mean while the ts-11 and killed vaccine decreased shedding of field strain from 108.1 and 108.6 to 105.1 and 105.8 CFU respectively at 14 days post challenge. In this study, rt-PCR had ability to identify and quantify of two types of vaccines (F and ts-11) and field strain.

66-73

Authors: Qureshi S, Musadiq Khan H, Saleem Mir M, Ahmad Raja T, Alam Khan A, Ali H and Adil Sh.

A biological trial was conducted on commercial chicks during the winter months (December and January). Day old commercial meat type broiler chicks (273) were procured from a reputed source. Cold conditioning (20C to 80C) at third and fourth day of age for 3-4 hours was provided to 78 birds. These early cold conditioned birds were kept separate until distributed into respective treatment groups (fifth and sixth). At the end of second week, the chicks were individually weighed, distributed into 7 treatment groups of 3 replicates with 13 chicks in each replicate. Cold challenge @ 20C to 80C for 8 hours was provided from third week of age to sixth week of their age for all treatment groups except first and fifth treatment groups. The broiler birds in the treatment groups T1 and T5 were reared under normal temperature conditions (250C). Treatment group first (T1) was kept as control group. Antioxidant Vitamin E 250 mg per kg of feed was supplemented to the basal diet in the third treatment group. Chromium 0.1 gram per kg of feed was supplemented to the basal diet in the fourth treatment group. Chromium 0.2 gram per kg of feed was supplemented to the basal diet in the seventh treatment group. The data on individual body weight of the experimental birds and the cumulative feed consumption and feed conversion ratio on group basis were recorded at weekly intervals. Deaths were recorded daily and all dead birds were necropsied to identify ascites syndrome. There was no significant (p< 0.05) difference in the average body weight and body weight gain among various treatment groups throughout the experiment period. The cumulative feed consumption showed significant (p< 0.05) difference among various treatment groups throughout the experiment period. Highest feed consumption (p< 0.05) was observed in broiler chickens reared under cold conditions when compared with broiler birds reared under normal temperature conditions. Among the cold challenge treatment groups (T2, T3, T4, T6 and T7), there was significant (p< 0.05) improvement in feed conversion ratio (FCR) in the treatment groups T6 (early cold conditioning birds exposed to cold stress) and T7 (supplementation of chromium 0.2 g/kg of feed to birds exposed to cold stress). Among different treatment groups in general best FCR was observed in treatment group T5 (early cold conditioning group reared under normal conditions) followed by T1 (control group reared under normal conditions). At the end of the biological trial ascites linked mortalities showed significant (p< 0.05) difference among various treatment groups. There was no mortality reported in treatment groups kept under normal temperature conditions (T1 and T5). Highest ascites related mortality (23.07%) was observed in treatment group in which cold stress was provided and no measures were taken to alleviate the effect of cold stress on broiler birds (T2). The Vitamin E supplementation in the diet of broiler birds reared under cold stress (T3) showed significant (p< 0.05) reduction in ascites related mortality (10.25%).

25-30

Authors: Rasooli R, Sheibani H, Kheirandish R and Rohollahzadeh H.

Hepatic damage in poultry occurs either due to metabolic or nutritional disturbances or chemical intoxication. The absence of reliable liver protective drugs and also consumption of broiler meat, limit us in usage of chemical hepatoprotective agents. The aim of this study is to evaluate the protective effects of CichoriumIntybus (CI) extract in paracetamol-induced hepatotoxicity in broiler chicks. One-day-old Ross chicken broilers were divided into four groups. One group was kept as normal and liver damage were induced in other 3 groups by oral administration of 1 ml/kg body weight of paracetamol for four successive days. Of 3 intoxicated groups one was kept as control and two different medicinal plants extracts were administered 0.2 g/kg of CI and 0.4 g/kg of CI extract. The medicinal plant was administered orally for 14 days after paracetamol administration. Then the blood samples were collected and the chicks sacrificed to histopathological examination. Serum liver markers and histopathological assessment of the livers revealed that Cichoriumintybus has protective activity against hepatic damage specially at a dose of 0.4 g/kg body weight and exhibited anti-hepatotoxic activity in broilers. The present study showed that administration of Cichoriumintybus extract at the doses of 0.2 g/kg/day and 0.4 g/kg/day respectively to Paracetamol intoxicated broilers, mitigates liver toxicity and liver histopathological changes.

37-43

Authors: Jabbar A, Riaz A and Allah Ditta Y.

Eggs weight loss during storage has been documented well due to water loss. The single stage incubators are designed to use egg water loss as humidity source during incubation. In this experiment one week old eggs (n=430840) were collected in Salman hatchery, Pakistan and divided into two groups, group A was (eggs n=215420) immediately shifted to incubators before 10 hours of incubation to avoid further weight loss) and eggs from group B (n=215420) was stored in eggs room at 23.80 C and 65% humidity with 2cfm/1000 eggs ventilation for 10 hours. After 10 hours of storage both groups were pre-heated at 80 0F for 5 hours leading to incubation conditions. Hatchability % (84.502±2.9221, 84.217±3.0279) candling% (6.5418±0.5605, 6.7682±0.5705) dead in shell% (6.5418±2.3112, 6.7682±2.3702) and hatch window (hours) (22-24±0.142, 26-28±0.1324) were significantly better for A compare to B respectively, Water loss% (11.556±0.1399, 11.545±0.1486), chick yield% (68.835±0.0926, 68.818±0.0928) and chick waste (gram) (19.67±1.721, 19.69±1.7653) were insignificant due to same incubation conditions inside incubator. The water loss from eggs of group A retained inside incubator have an impact on embryonic mortality including the duration of first and the last chick that hatches out. So, the deleterious effects of storage can be avoided by shifting the eggs inside a single stage incubator before 10 hours of incubation including pre-heating.