Spei Domus

1-14

Authors: Torres-Ruda, Felipe, Torres-Cruz, Mónica, Joya-Cárdenas, Carlos A., Castellanos-Duarte, Frank A.

Introduction:Goat milk is recognized as a food with compounds of high biological value given its composition and intrinsic characteristics, reaching high recognition and commercial value. However, the production sys-tems of the region have been developed in isolation, without technical support, absence of records and low technological inclusion, ignoring limited quality values through regulatory standards that regulate their produc-tion and extension. Therefore, the objective of this work was to recognize characteristics of composition and hygienic quality of goat’s milk in farms located in the García Rovira province in the department of Santander. Methods: Analysis of the physicochemical parameters density (g / cm3), fat (%), non-fat solids (MSNF) (%), protein (%) and cryoscopic point (°C) using the brand Milkana Kam 98-2A ultrasonic milk analyzer Ekomilk ®. Results: The means values for the physicochemical variables were: 4.44 ± 1.66%, in fat, 3.31 ± 0.24% in protein, 8.77 ± 0.65% in SNG, 1.031 ± 0.03 g / cm3 for density and, -0.561 ± 0.06 ° C for the cryoscopic point; the TRAM test also showed that 73% of the milk is classified as grade II and III, which is accepted for human consumption, in addition 80% of the animals of the UP evaluated were negative to the CMT test. Conclusions: These specific data with fundamental referential information that can help in structuring aspects of a regulatory nature for the consumption of goat’s milk in the country.

1-16

Authors: Ingris Yohana Hernández Martínez, Jenny Johanna Pacheco López, Ana Paula Castro Santos, José Nelio de Souza Sales

The purpose of this article is to provide the reader with knowledge about the growth relationship between the future dominant follicle FD, the subordinate follicle (FS) and the intraovarian factors that influence follicular divergence in cattle. Development. This review begins by presenting the importance of follicular divergence, when a significant difference is observed in the growth rate between the first two follicles. Thereafter, the aspects that influence this divergence are covered: presence of the corpus luteum (CL), its relationship with the dominant follicle (DF) and plasma progesterone concentrations (P4), in addition to the endocrine and molecular factors related to the follicular divergence. It is concluded that as more in-depth research is carried out, new factors that influence this process are discovered.

1-16

Authors: Pablo Antonio Fariña Sirandoni, Rodrigo Ambrosio Pulgar Aguila, Alejandro Ignacio Molina Cofré

Introduction: platelet rich plasma (PRP) usage has acquired great relevance within the last few years, which has demonstrated its usefulness in the treatment of some pathologies. Up until now, there is not enough information on efficient obtention processes that warrants a wished minimal concentration for therapeutical purposes. This study compared five known protocols for PRP obtention and stablished the best performance to determine which protocol offers the greatest number of platelets with common resources within the clinic. Methodology: five standard protocols recommended by international literature were compared by its capacity to accomplish larger amounts of platelets. Canine blood with a normal hemogram was used to evaluate each protocol by counting each PRP number. Results: a platelet count was carried out and it was found that 163% for protocol C, followed by protocol B, E, D and A with 96%, 8.62%, 6.38% and 4.47%, respectively. Conclusions: there is a great variability between platelet count within each protocol. In this case, protocol C gave the best result, being the one that demands more revolutions and time for the obtention of PRP.

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Authors: Maura Cruz-Fierro, Laura Jaramillo-Meza, Clara Inés Espitia-Pinzón, Rafael Pérez-González, Anabelle Manzo-Sandoval, Fernando Díaz-Otero

Introduction: bovine tuberculosis (bTB) continues to be a serious public and animal health issue, specially in developing countries, in which prevalences in bovine dairy systems can reach numbers above 40%. Consequently, its control must be grounded in whole alternatives for its animal source in order to prevent transmission between animals and human beings from a “one health” perspective. Vaccination is considered the most viable option to reduce its incidence; nonetheless, to achieve this purpose successfully is necessary to determine the conditions under which vaccine must be applied, and understand the immunological mechanisms giving resistance against mycobacterium bovis, since its an intracellular bacteria it is desirable that vaccines induce a Th1 type response, characterized by gamma interferon production (IFNγ), which is a tumor necrosis factor alpha (TNF) and interleukin 12 (IL-12), cytokines responsible for macrophage microbicide activation and effect. This study evaluated vaccination effectiveness of BCG vaccine and proteic extract filtered from a m. Bovis (CFPE) culture as immuno agent against bTB, through monitoring of IFNγ production, evaluation of cytokines Th1 expression (IFN-g, IL-2), Th2 (IL-4, IL-10), and the presence of macroscopic lesions towards necrosis after the challenge. Methodology: 24 Holstein-Friesian calves of six months old, negative to different bTB immunodiagnostic tests, were devided in four groups (6 calves/group): not vaccined group, BCB vaccined, vaccinated with CFPE m. Bovis AN5 and vccinated with CFPE, but with previous IFNγ treatment. IFNγ, IL-2, IL-4 and IL-10 cytokine expression was evaluated through RT-PCR and represented as the percentage of relative intensity to corresponding bands with respect to internal positive control (b-actin), using LabWorks 4.0 software. Immune cellular response was evaluated through IFNγ production in cell culture estimated with bovine and avian PPD in different periods. Results: cytokine expression was different between vaccinated and control groups, both in the postvaccinal and postchallenge periods. Expression of IFNγ in vaccinated group with BCG was higher in front of in vitro antigen stimulation with bovine PPD and with DipZ antigen in the evaluated postvaccination period. Immunized groups with CFPE, expression of IL-4 was significant during that period, specially in group treated with IFNγ. After challenge, production and expression of IFNγ increased in all groups; being 200 times higher for the group treated with IFNγ with different antigen. In this group, higher expression of IL-2, IL-4 and IL-10 was also significant, especially IL10, a known antagonist of cellular immunity. In fact, this vaccinated group presente the highest number of lesions to challenge; being, nonetheless, less severe than the ones in the control group. Conclusions: the vaccinated group with BCG registered the lower number and degree of lesions, correlated with a significant IFNγ expression and production. Previous treatment with IFNγ in the immunized group with CFPE showed an adverse effect in the stablishment of a protective immunity due to a negative modulation from IL-4 and IL-10. According to the results, BCG is a better immune agent in the study, so that its use as an alternative for disease control is promising.

1-20

Authors: Gabriel Serrano-Díaz, Orlando Villa-Mesa

ntroduction: in the livestock sector it is usual to attribute the sex of the offspring that are born to the influence of some external and external factors to the animal. This work explores and supports some evidence on the possibility that these factors have a real association on the sex of the offspring. Methodology: the data recorded through private professional practice by one of the authors for 16 years (2000-2015) in 23 farms corresponding to 18,124 births (1,108 with service registration) were used. The Excel and Panacea 2 programs were used. Significance tests were performed using one-way analysis of variance and chi-square tests in contingency tables. Results: the percentage of females born was lower and there was a farm with a high predominance of the proportion of these. The marked seasonality of births was noted throughout the year. No differences were observed in the proportions of females and males born inherent in the production system or the time of year of birth. Significance was observed for a higher percentage of births in the crescent moon phase, and in this same, a higher proportion of males in the births. The days from lunation to birth did not show differences in the numbers of births and proportions by sex. No differences were observed in the proportions of conceptions by sex due to the lunar phases or days of the lunation at the time of conception. For the duration of gestation, significance was observed in the differences determined by the factors “farm” and “production system”; No influence was observed of the sex of the calf that was gestating or of the variables month, moon phase and lunation day in which both conception and delivery occurred. Conclusions: the percentage of males conceived and born is slightly higher than that of females; None of the factors analyzed can be used to increase the percentage of conceptions of either sex, but an increase in both the total proportion of births and males in the births registered in the crescent moon phase is noticeable.