International Journal of Applied Sciences and Biotechnology

123-127

Authors: Ouation Souleymane Soro, Vincent Kadjo, Koffi Sylvanus Aubert Yao, Moussa Kimse

The aim of this work is to test the effect of green forage Ipomoea batatas on young rabbit (Oryctolagus cuniculus) digestive health and growing performance. Two group (T and I) of 15 rabbits were made. These animals were weaned at 35 days of age and slaughtered at 70 days. The mean weight per group was 500 ± 20 g. Control group T was feed only with industrial pelleted Ivograin®. Experimental group I was feed with the same pelleted diet and green forage I. batatas in the cage. Results showed that the growing performances in group I were lower than control. Group I daily gain was 0.89g lower than T (P<0.01). Fed intakes was 1.05 times lower with forage than control (P<0.01). For sanitary risk index (mortality + morbidity) was 2 part higher in group I than control (14 vs 7, P<0.01). However, the nutrient digestibility was higher with I. batatas group than control. Digestibility of DM, OM, CP, NDF, ADF and Hc was 26.68, 23.43, 9.67, 18.56, 18.74 and 16.46 respectively higher with group I than control (P<0.05). We can conclude that, associate green forage I. batatas with pelleted diet was not increased the young rabbit growing performances and health except nutrients digestibility.

135-142

Authors: M. Paudel, B. Bohara, T. Prasai, S. Bhattarai, T.R. Gompo

The dairy sector in Nepal has a high potential for growth, but unfortunately, its progress is impeded by diseases such as mastitis. The purpose of the current investigation was to identify the predominant bacterial species in mastitis-affected cattle milk and to ascertain the most efficacious antibacterial treatment against the bacterial isolates. The study was conducted from 17th August 2022 to 12th February 2023 in lactating cattle of the Kirtipur municipality and Chandragiri municipality of Kathmandu district, Nepal. A total of 640 milk samples from 160 cattle were collected randomly from cattle farms of Kirtipur and Chandragiri municipality of Kathmandu district. Initial screening for mastitis was conducted on collection sites by the California Mastitis Test (CMT) and further bacterial isolation and identification were carried out at the Central Veterinary Laboratory using standard bacterial techniques and the antibiogram were evaluated based on CLSI guidelines. Among 640 samples collected from 160 cattle, 3.91% (25/640) tested CMT-positive at the individual quarter level, whereas the prevalence at the cow level was 15.62% (25/160). The major bacterial species isolated, in subsequent culture of CMT positive samples, were Staphylococcus spp. (10, 40%), E. coli (6, 24%), Streptococcus spp. (5, 20%), Enterococcus spp. (2, 8%), Bacillus spp. (1, 4%), and Klebsiella spp. (1, 4%).. Antibiogram study reveals that major gram-positive bacteria, Staphylococcus spp., Streptococcus spp., Enterococcus spp., and Bacillus spp. were highly sensitive to Ampicillin, Florfenicol, and Gentamicin while the major gram-negative bacteria, E. coli, and Klebsiella spp. were highly sensitive to Florfenicol, Gentamicin, Trimethoprim/Sulfamethoxazole and Ceftriaxone. Hence, early diagnosis and careful monitoring of antimicrobial susceptibility before therapeutic intervention are essential to prevent high economic losses due to mastitis and to mitigate the potential risk of antimicrobial resistance in the livestock population.

152-157

Authors: Ashok Sah, Prakash Gurung

The experiment was conducted in Ratuwamai Municipality, Morang, Nepal to assess the impact of various cropping systems on soil fertility and nutrient status. Four different cropping systems (Rice-Wheat-Rice, Rice-Mustard-Maize, Rice-Maize and Rice-Mustard-Rice) were selected as treatments and all treatments were replicated five times in Randomized Complete Block Design. Soil samples from 0-15 cm depth were collected from each site and evaluated for soil pH, Soil Organic Matter content (SOM), Total Nitrogen (N), available phosphorus (P), available potassium (K), sand silt and clay content. All the tested parameters except Soil N content, sand content and clay content were found to be significantly affected by the cropping system. The soil in all four cropping systems were found acidic in nature with pH ranging from (4.18-4.48). The Soil Organic Matter was recorded highest (1.84%) from Rice-Maize based system and lowest amount of Soil Organic Matter was observed in Rice-Mustard-Rice based system. The highest N content (0.19%) was recorded from the Rice-Mustard-Maize cropping system and the lowest N content (0.02%) was recorded from Rice-Wheat-Rice cropping system which is statistically similar to Rice-Mustard-Rice based cropping system. The highest P content (163.95kg/ha) was recorded from Rice-Mustard-Maize and the lowest (75.28kg/ha) was recorded from Rice-Mustard-Rice based cropping system. The highest K content (169.18kg/ha) was recorded from Rice-Mustard-Rice cropping system and the lowest K content (57.76kg/ha) was observed in Rice-Wheat-Rice based system. The highest silt content (48.38%) was recorded from Rice-Mustard-Maize cropping system. The result indicated that the Soil Organic Matter, Nitrogen and Potassium were found to be deficit nutrients in all the cropping system.