Visnyk of Dnipropetrovsk University. Biology, medicine

9-15

Authors: O. M. Alekseenko, T. M Polishko, A. I. Vinnikov

The direct comparative facts of different options of inoculum production of Plearotus ostreatus:classical, tissue, with the use of «grain», active and fermentative mycelium are presented. The advantages and shortcomings of each method have been shown, that gives the possibility of chosing the most optimum variant for already formed grow conditions. The methods of mycelium production on the grain substrate and also with the use of the active material demand considerable expenditure for the equipment and absolutely sterile conditions that is very difficult to achieve. The improved technological possibilities of mycelium production by a new fermentation way promotes rationalisation and profitability, that cause reduction of product price

22-27

Authors: S. I. Aliyeva

The results of the long term research on the number and the species composition of the phyllosphere’s yeasts (epigenous yeasts) of the plants in the northern, southern and subtropical regions of the Republic of Azerbaijan have been generalized. The obtained results show that the number and diversity of the yeast communities naturally decrease in different substrates, which correspond to different stages of the plants development. A set of dominant species is characteristic for each stage of the development. High yeast diversity is peculiar to forest biogeocenos rather than other ones. We identified 48 types of yeasts in phyllosphere and associated substances, 35 of which we were able to identify by morphological and physiological characteristics. Most of the identified yeasts were minor species, whose occurrence did not exceed 0.5% of the total specified yeasts. The results show that only 25 species of yeasts are permanent habitants of phyllosphere and alltogether they account for 90 % of the total yeast population.

34-40

Authors: T. E. Vygrayenko, I. E. Sokolova

Gas-producing bacteria were isolated from natural media: soil and compost extracts. The properties of the obtained microorganisms cultures as destructors of organic substances were studied. Studied bacteria were cultivated in experimental semi-synthetic media with saw dust and carboxymethyl cellulose. A growth at different pH values, a dynamics of growth, an amount of utilizated sugar, a volume of released gas during cultivation were studied. Most intensive growth of gas-producing cultures has been observed in a medium with CMC and the ability to gas producing was more manifested in the bacterial culture isolaited from the compost.

50-54

Authors: A. M. Goncharov, V. G. Gavriljuk, E. V. Matseljukh, A. I. Vinnikov

It was established, that production of proteolytic enzymes from yeasts is effected by sources of Carbon, Nitrogen and energy. The impact of different organic substances to proteo-, fibrino-, gelatine- and haemoglobinolytic enzyme activity from Yarrowia lipoytica 2061 is established. Positive effect of addition galactose and gelatin with glycine in cultural liquid is revealed. Optimal temperature, pH and other physical parameters for getting proteolityc enzyme complexes is defined.

60-65

Authors: О. А. Zemliany

Microelements accumulation features are considered in micromammals’ organism taken from different degrees transformation biogeocoenosis of Dnipropetrovs’k region. Coefficients of accumulation are defined in different organs and tissues. Accumulation of heavy metals in the micromammals’ organism in different ecosystems, with a different technogenic contamination is heterogeneous. Organs which defined as concentrators are spleen, heart and gonads. They have primary accumulation of heavy metals. Organs–deconcentrators are those which have active extraction of microelements (liver, kidneysand and intestine). Tendency to the decline of microelements accumulation intensity in animals from strongly transformed biogeocoenosis are stated. Formation of the special barriers of accumulation and excretion from organism by organs–deconcentrators testifies to making an adaptive mechanism at the level of physiology-biochemical processes and, first of all, the metabolism intensification.

71-77

Authors: O. M. Kunakh

The network approach is proposed to study the animal community with herpetobiont spiders of Dnipropetrovsk region as an example. The network representation is an alternative of an ordination paradigm in community structure description. The advantages of the network approaches in animal community analysis are shown. The possible directions of the herpetobiont spiders community formation of the steepe zone of Ukraine have been found. The effect of the forests on the steepe animals community is shown.

83-88

Authors: M. V. Markina, О. V. Vyatkin, V. P. Lyashenko, А. І. Rudenko

Connection between the pathologies of alimentary canal and the changes of concentrations of microelements in human saliva is under consideration. The decrease of the cations content in saliva under forming and developing the pathological process in gastrointestinal tract is shown.

97-102

Authors: N. V. Nedzvetskaya, N. I. Таshevskaya

There was investigated protective effect of chronic melatonin injection on the development of cognitive deficit in rats with streptozotocine induced diabetes. There were observed decrease of astrogliosis and of cognitive deficit in animals that were injected with melatonin. Presented results are evidence that melatonin can prevent the development of cognitive deficit in diabetic animals by decreasing oxidative stress level and astrogliosis inhibition.

107-111

Authors: N. I. Tashevska, V. S. Nedzvetsky, L. K. Perstneva

The advantage of donor-recipient pairs’ selection HLA for kidneys transplantation by the second grades HLA-DR determined by a method of DNA typing as compared with the selection based on the antigens of the first grade has been studied. It has been found that genetic typing is highly sensitive and specific. The results of the research show that the selection by the antigens HLA-DR is more efficient.