Journal of Innovative Biology

5

Authors: Shefali Kansal, Pankaj Kumar Jiloha*

Pus is a whitish yellow or yellowish substance which is made of dead leukocytes and dead bacteria. Pus infection, due to various bacteria has a significant impact on public health in the region so early diagnosis and appropriate treatment remain important. The present study was carried out for a period of 3 months (February to April 2014) in Microbiology Laboratory of General Hospital Panchkula (Haryana), to isolate, identify and screen various bacterial pathogens present in pus sample and to determine their antibiotic sensitivity and resistance pattern against the commonly used standard antibiotic. A total of 63 pus samples were collected and analyzed. Out of total 63 samples, 42 bacterial isolates of 6 species were isolated which included 2 species of gram positive bacteria and 4 species of gram negative. 21-30 age groups were found to be the most vulnerable age group in both male and female. In-vitro study determine that the most common gram +ve bacterial pathogens isolated and identified from pus sample were Staphylococcus aureus and S. epidermidis, both are facultative anaerobe but S. aureus was found to be most predominant followed by S. epidermidis. During antibiotic susceptibility pattern, the most sensitive antibiotic was Vancomycin (100%) while the least effective antibiotic was Amoxicillin (35%) followed by Penicillin (36%) respectively. Our study reveals that bacterial pathogens showed resistance to most of the antibiotics. Penicillin, Ampicillin, Ciprofloxacin, Ofloxacin, Gentamycin antibiotic were showed high resistance to prevent from gram positive bacteria. So, it may be concluded that the high prevalence of S. aureus infection treated with the use of appropriate antibiotic i.e. the use of Vancomycin.