Bìofìzičnij Vìsnik

5-9

Authors: M.A. Bondarenko, V.G. Knigavko, N.O. Gordiyenko, O.V. Zaytseva, T.R. Kocharova

Carcinogenesis is subject of the research. The research aims at creating the mathematical model of carcinogenesis allowing assessing the distribution in human population of the genes which when damaged lead to oncology diseases. The main task is to build a probability mathematical model describing the quasistationary equilibrium of two contrary processes, and namely: 1) the process of reduction in population of the number of the aforesaid genes due to their mutative damage; 2) increase in population of the number of these genes due to the fact that persons with a few genes of the kind in their genotype acquire oncological diseases with higher probability at early stages of their lives and do not manage to reproduce themselves before they die, and so the growth of the total population size is more due to the reproduction of individuals with a high number of the a-genes. Assessment of the distribution of these genes in the population was carried out by determining the probability that a randomly selected individual from the population has one of the possible values (according to the literature, from 0 to 8) of the aforementioned genes.

21-27

Authors: D. M. Glibitskiy, G. M. Glibitskiy, M. A. Semenov, A. V. Shestopalova

Analysis of the patterns on the dried films of biological fluids is a promising avenue of medical diagnostics and biosensors development. The aim of this work was to study the effect of iron and copper ions on the quantitative characteristics of textures and zigzag patterns (Z-structures) observed on the films of saline solutions of bovine serum albumin (BSA). In the experiment, 0.5 mg/ml BSA, 20 mM/l NaCl, 0.05 mM/l CuCl2 and 0.2÷0.05 mM/l FeCl3 were used. For textures, the area and fractal dimension were estimated, and for zigzag patterns, area and the specific length of the Z-structures were estimated. The effect of iron and copper ions on the geometrical parameters of Z-structures was analyzed. Spatial distributions of the fractal dimension of texture and the specific length of Z-structures were analyzed. It was shown that using the product of all four parameters increases the sensitivity of the method compared to using only one of the parameters. The method can be used in pharmacology and medical biochemistry for screening drugs and chemical compounds by their degree of influence on biopolymers.

34-45

Authors: M. A. Popirny, O. T. Nikolov, E. V. Skrylnik

The purpose of this work lies in studying the composition, physical and chemical properties of the second fraction of the humic acid (HA2) extracted from the typical black soil, depending on the intensity of the soil tillage. Physical and chemical properties of HA2 have been studied by using ultraviolet (UV) spectroscopy, infrared (IR) spectroscopy and electron spin resonance spectroscopy (ESR). It has been demonstrated that increase of the tillage intensity of the typical black soil leads to decrease in the total carbon content and the HA2 content due to displacement of balance between the conjugated processes of humus synthesis and decay towards destruction. UV spectroscopy indicates intense absorption of humic acids in the near UV region, that corresponds to the donor-acceptor mechanisms π-π* electrons of double bonds. Maximum absorption has been observed for the HA2 isolated from the black soil tilled by plowing. Moreover, the highest content of oxygen-containing functional groups and low quantity of aliphatic structures has been observed in the same sample. It has been shown that the nature of paramagnetic centers of the HA2 represented by chelate paramagnetic Fe3+-complex formed by rearrangement π-electron system of double bonds, depending on the tillage intensity of the typical black soil. It has been found that plowing of the typical black soil enhances the number of paramagnetic centers in HA2.