International Journal of Medical Laboratory

4

Authors: Zakieh Rostamzadeh *, Nasim Valizadeh, Mahshid Mohammadian

Background and Aims: Human T-lymphotropic virus (HTLV) is a human retrovirus which has been known to cause adult T-cell leukemia/lymphoma and some other inflammatory disorders. Patients with hereditary bleeding diseases are at high risk for these viruses. In this study, we evaluated serological HTLV-I/II infection among these patients in west Azerbaijan of Iran. Material and Methods: We studied 50 hemophiliacs including 43 males and 7 females. Serum samples were screened for Anti-HTLV-I and II using enzyme-linked immunosorbent assay. Western blot method was applied on borderline results for confirmation. Results and Conclusions: All studied patients were seronegative for HTLV-I/II and serologic evidence of HTLV I/II infection was not found in any of patients. Based on our results, blood donor population of Urmia must be increased to achieve more accurate results.

7

Authors: Amir Cheraghi, Shahla Mohammad Ganji *, Bita Najmi

Background and Aims: Recently it has been proved that some of Enterobacteriacea like Klebsiella pneumoniae, which carry PKS islands, damage dsDNA by encoding Colibactin genotoxin; and finally they induce some apoptosis in damaged mucosal cells. This study aimed to isolating the clbN and clbB genes, which are the markers of the PKS genomic island, from PKS cluster in these bacteria Materials and Methods: In this study, 110 biopsies were obtained from colorectal cancer patients referred to the clinic of Imam Khomeini Hospital. Then, all samples were cultured in LB medium for bacterial isolation. In the next step, Klebsiella peunomoniae was detected by biochemical and microbiological tests. Finally, the genomes were extracted by boiling method and then were amplified by PCR with specific clbN and clbB gene primers. Results: Our findings have showed 30 Klebsiella pneumonia isolated out of 110 biopsies (27.3%) from patients with colorectal cancer; in which all of biochemical and microbial experiments including Gram-negative staining confirmed this bacteria. Also, 23.23% frequency of clbN were positive, 20% frequency of clbB were positive genes in the isolated Klebsiella pneumonia, and 13.33% frequency were positive for both clbB and clbN genes as simultaneous. Conclusions: It is expected to find a significant correlation between Klebsiella bacteria which carries PKS genes and colorectal cancer by increased number of samples. The lower frequencies for Klebsiella with clbB and clbN positive strain in patients with colorectal cancer were shown in European countries based on the results of this study rather than the similar studies.

7

Authors: Reza Dolatyari Eslami, Asghar Tanomad *, Jamal Hallajzadeh, Mehdi Ariana

Background and Aims: Probiotics are defined as live microorganisms that are beneficial to human health. Probiotics are commonly found in some foods such as dairy products or complementary tablets. A recent research shows that probiotics have a significant role in diabetes type 1 and 2 treatment. Among the variety of lactic acid bacteria, Lactobacillus strains are well known in probiotics for their different properties. The aim of this study is to investigate the effect of Lactobacillus delbrueckii PTCC1057 on diabetic mice blood sugar. Materials and Methods: sample mice were selected from the age range of 6 to 8 weeks. Then for converting the normal mouse to diabetic type, 180 mg/kg streptozotocin was injected intraperitoneally. 10 days later, the blood sugar of the mice was measured and the mice with higher than 200 mg/dl of blood sugar were considered as diabetic mice. Finally, the diabetic mice were divided into two groups of experimental and control for next investigation. For a survey of probiotics effect on blood sugar, an amount of 3×106 CFU of L. delbrueckii was fed by gavage feeding approach daily during 4 weeks and the blood sugar level was determined at the end of the week after streptozotocin injection. Results: the result showed that the diabetic mice blood sugar level significantly decreased conspicuously by feeding L. delbrueckii in comparison to diabetic control group. Conclusion: The result of this study suggests that L. delbrueckii can be impressive in diabetes treatment. However, another clinical study is needed for proving the exact effect of this probiotic on diabetes.

9

Authors: Niko Rostaei Rad, Mahmood Vakili, Javad Zavar-reza *, Sona Rezaie, Ahmad Reza Shirvani

Backgrounds and Aims: Thyroid hormones have a crucial physiological role to maintain balance of metabolism of body. Thyroid hormones have an important role in metabolism and proliferation of blood cells. Thyroid gland also has a vital influence on erythropoiesis by induction of erythropoietin secretion and proliferation of erythroid progenitors. In the present study, it was tried to evaluate the effect of hypothyroidism on hematological parameters and also body iron store. Materials and Methods: The research population consisted of 90 subjects divided into two groups: 45 people with hypothyroidism and 45 age-matched healthy individuals. This study checked the amounts of total triiodothyronine (T3), total thyroxine (T4), thyroid stimulating hormone (TSH), and some iron status and hematological parameters. Results: The mean TSH levels were significantly increased in hypothyroid patients compared to control group. On the other hand, the levels of T4 and total iron binding capacity, serum iron, ferritin, Hemoglobin, and Hematocrit were significantly lower in hypothyroid patients compared to control group (p<0.05). However, there was no significant difference in the mean of T3 between hypothyroidism and control. Conclusion: Our data suggest that alterations in thyroid status change serum iron metabolism and hematological index. Hypothyroidism is often associated with anemia,depleted body iron store. As there is no significant clinical manifestation of hypothyroidism at earlier stages with anemia, it is advisable to routinely investigate it for early detection allowing its early management.