Animal Production

1-12

Authors: Basengere Ayagirwe, Felix Meutchieye, Appolinaire Djikeng, Robert Skilton, Sarah Osama, Yacouba Manjeli

Although domestic cavies are widely used in sub-Saharan Africa as a source of meat and income, there are only a few studies of their population structure and genetic relatedness. This seminal study was designed with the main objective to assess the genetic diversity and determine the population structure of cavy populations from Cameroon to guide the development of a cavy improvement program. Sixteen microsatellite markers were used to genotype 109 individuals from five cavy populations (Wouri, Moungo and Nkongsamba in the Littoral region, and Mémé and Fako in the Southwest region of Cameroon). Twelve markers worked in the five populations with a total of 17 alleles identified, with a range of 2.9 to 4.0 alleles per locus. Observed heterozygosity (from 0.022 to 0.277) among populations was lower than expected heterozygosity (from 0.42 to 0.54). Inbreeding rates between individuals of the populations and between individuals in each population were 59.3% and 57.2%, respectively, against a moderate differentiation rate of 4.9%. All the tested loci deviated from Hardy-Weinberg equilibrium, except for locus 3. Genetic distances between populations were small (from 0.008 to 0.277), with a high rate of variability among individuals within each population (54.4%). Three distinct genetic groups were structured. This study has shown that microsatellites are useful for the genetic characterization of cavy populations in Cameroon and that the populations investigated have sufficient genetic diversity that can be used to be deployed as a basis for weight, prolificacy and disease resistance improvement. The genetic of diversity in Southern Cameroon is wide and constitute an opportunity for cavy breeding program.

21-27

Authors: Siti Darodjah Rasad, Rangga Setiawan

Aim of this study was to examine the characteristics cytology of mucous cell-,temperature- and pH vagina during estrous cycle in local sheep. 31local sheep were synchronized with vaginal sponge consist of 20 mg progesterone hormone before carried out observations of cytology of cells from the vaginal mucose through vaginal swabs, temperature and pH of the vagina. Vaginal swabs were collected daily at 7 am for a weeks.Vaginal temperature and pH measurement is carried out twice a day, at 07.00 am and 15.00 pm for a weeks after vaginal swabs. Smears of the swab were then prepared on glass slide and they were stained with Giemsa. Vaginal epithelial cells; Parabasal, intermediate and superficial cells were counted and their percentages during pro-estrous, estrous and di-estrous were determined. Di-estrous was characterized by the absent of superficial cells in the epithelial vagina. Pro-estrous was characterized by the increasing progressively of intermediate/superficial cells in epithelial vagina, whereas estrous was characterized by the presence of superficial/cornification cells in most epithelial vagina. Based on the dominance of superficial cell, the number of sheep identified as estrous is highest on third day, with 52%. Observation on vaginal temperature also resulting that the highest temperature values obtained on the third day of 39,08±0.28°C. It could be effected of the vaginal pH during the observation. Underthe influence ofestrogen, the epithelial vaginalcellssynthesizeand accumulateglycogenin large quantitiesdepositedin the lumen ofvagina. Vaginal bacteriametabolizethe glycogenformlactic acid, which causesvaginal pHis low.The pH conditions prevent from pathogenic microorganisms and fungi. Increased estrogenal so cause cell proliferation through the thickening of the epithelium lining of the vagina so that the cells differentiate.Increasing of glycogenin the superficial cells, and ceratin cells found in the cytoplasm of cells, as well as acidic pH in the lumen vagina causing changes in the chemical composition of each cell (Zaid, 2011).It can be concluded that during estrous phase, cytology of vaginal mucose cell is dominated by superficial cell and vaginal temperature is increasing as well as pH of vagina.

37-45

Authors: Dwi Margi Suci, Zurrahmi Fitria, Rita Mutia

Fat, cholesterol and fatty acid profile of breast and thigh meat of 12 Muscovy ducks (6 drakes) aged 8 weeks from the local farm in Bogor, West Java were analyzed in thus study. In general, Muscovy ducks were fed with rice bran twice a day and free-ranged in the backyard. The nutrition of rice bran is varied in crude protein, crude fiber, fat, and ash content. Muscovy ducks were slaughtered and the percentage of carcass weight, fat, cholesterol and fatty acid profile of the breast and thigh meat were measured and analyzed. The result showed that the breast meat had higher (P<0.05) ω3 total fatty acid EPA (C20:5ω3) but DHA (C22:6ω3) and linolenic acid (C18:3ω3) were not significant compared to that of thigh meat. Concentration of linolenic acid in breast meat ranged from 0.26 to 0.51% of fat and 0.35 to 0.39% of fat in thigh meat. Linoleic acid (C18:2ω6) in breast and thigh meat was 7.64 - 10.73% and 5.17 - 13.93 % fat, respectively. DHA concentration ranged from 0.13-1.31% fat in breast meat and EPA in thigh meat was approximately 0.08-0.32 % fat. EPA was significantly higher (P<0.05) in breast meat (0.03-0.28 %) than thigh meat (0.05-0.08 %) The study produced ω 6:3 ratio ranged 6.50-24.14 and 8.94-31.64 in breast and thigh meat, respectively.

55-60

Authors: Irma Badarina, Heru Dwi Putranto, Endang Sulistyowati

The objective of this research was to evaluate the anthelmintic efficacy of the ethanolic and aqueous extract of coffee husk fermented by Pleurotus ostreatus against Ascaridia galli. In vitro study of anthelmintic activity was conducted by counting the number of paralyzed worm body of Ascaridia galli during 24 hours in petri dish containing different levels of ethanolic and aqueous extract of fermented coffee husk, namely 0%, 1%, 2%, 3% and 4% (w/v) and compared to piperazine 0,5% (w/v) and NaCl 0,9%. The powdered samples of fermented coffee husk were macerated in the 30% ethanol solution with the ratio samples to solution = 1:3. In aqueous extract, samples to solution ratio was 1 : 7. The extract of fermented coffee husk possess phytochemical compunds which responsible for anthelmintic activity. The anthelmintic activity of ethanolic extract was better than aqueous extract. Fermented coffee husk ethanolic extract exhibited mortality of A.galli at concentration 2% while showed highest mortality at concentration 4%. Fermented coffee husk ethanolic extract have the potential to aid in the control of gastro intestinal parasites in animal