Cumbres

17 - 24

Authors: Fabricio Guevara Viejo, Leonidas Pacheco Olea, Fernando Pacheco Olea

Since 2008, the National System of Higher Education (SNES) has undergone a noticeable transitional process. This transition is the result of the implementation of some government policies which seek to achieve an enhancement in university teaching and research practice and their consistency with community involvement. Nevertheless, the outcomes obtained by the end of the 21st century, after an extensive process of institutionalization, forced the government to make the radical decision of applying certain processes of deinstitutionalization and re-institutionalization at the same time. Those processes resulted on a renewed institutional, cognitive, and social reality which finds some resistance in some academic areas as there are both practices and procedures that have been established over time by the inertia of the traditional system. We will explore and analyze some of the most relevant processes of institutionalization, deinstitutionalization, and re-institutionalization within the National System of Higher Education as well as how those processes create a reality which is built upon the expectations of stakeholders and the resignification that the actors involved in Ecuadorian universities give to this reality.

31 - 37

Authors: O. Cabrera, M. Pisonero, M. Rodríguez, R. Pérez, E. González, J. Pedroso, D. García, M. Cuello

Regulatory authorities recommend the use of immunochemical techniques to determine the identity of the antigens present on the conjugate vaccines. With the emergence of multivalent vaccines, it has become necessary to use immunochemical techniques using monoclonal antibodies to increase the sensitivity in the determination. The aim of this study was to establish the analytical conditions that would allow to use the Dot Blot as a technique to determine the identity of capsular polysaccharides from Streptococcus pneumoniae serotype 14 (PsC 14) and the tetanus toxoid (TT) used as carrier protein in multivalent conjugate vaccines. The MAb against the PsC 14 and TT were used; besides were used some batchs of monovalent conjugates of PsC 14; batchs of a heptavalent conjugate pneumococcal vaccine candidate with TT as carrier protein and batchs of TT, all produced at Finlay Institute of Vaccines. The results showed that for the determination of the antigenic identity were optimal volumes of 10µL of monovalent conjugate samples at a dilution of 1/10 (vol/vol) and equal volume for heptavalent vaccines. For MAb was demonstrated that 2µg/mL concentration of MAb against the PSC 14 and 2.5µg/ mL for MAb against TT were sufficient to successfully perform the determinations; while incubation times were adjusted at 37oC for 30min. The work allows us to conclude that working conditions in the laboratory were established to determine the identity of the capsular polysaccharides of S. pneumoniae serotype 14 and for tetanus toxoid as carrier protein in the heptavalent conjugate vaccines by Dot Blot method.

49 - 55

Authors: Nubia Lisbeth Matute Castro, Gloria Panades Ambrosio, Luis Cruz Viera, Ana Echavarria Velez, Verónica Bravo Bravo

A potentially functional tomato juice of high biological value using turmeric (Curcuma longa) as an antioxidant agent has been designed, evaluated and optimized. The formula of the selected product was prepared on a pilot scale. Based on the sensory analysis and physicochemical tests, the optimum formula with tomato concentrate was found; (18-20 °Brix) 7.5 to 8.3%, refined sugar 2.5%, salt 0.8%, xanthan gum 0.088%, dehydrated turmeric powder (7% humidity) and 0.066 water. The phenol content of the glass packaged product was of 171.4 mg of gallic acid/L, and antioxidant capacity was of 1187,0 µM Fe2+.