Annales Bogorienses

1-8

Authors: Dominggus Malle, Gina Garingan, Milagros Peralta, Maria Jamela Revilleza

Lipases [EC. 3.1.1 .3] are capable of hydrolyzing ester bonds of triglycerides and have received much attention from protein researchers after these enzymes have shown potential applications such us, in the manufacture of commercially important chiral drugs due to their enantioselectivity properties. A novel extracellular lipase produced by Aspergillus niger was purified and partially characterized. Using the culture medium as crude extract, the Lipase was purified to 25 .7 folds after Ultrafiltration and DEAE ion exchange chromatography. The enzyme was characterized to have optimum activity at neutral pH and at 30-35°C, while its kinetic parameters, Vmax and Km, were determined to be L80 U/mL/min and 0.2 mL , respectively. SDS-PAGE analysis revealed the presence of at least two bands (±87 and ±72 kDa), while native PAGE showed a single band. The two bands could represent subunits in a complex or isoforms of the enzyme. The use of the enzyme in th hydrolysis of a racemic mixture of 2-arylpropionic butyl ester analyzed after HPLC demonstrated an apparent predominant enantioselectivity for the S (+) enantiomer. Thus, this lipase is a promising enzyme for the chiral drug preparation of single enantiomer of 2-arylpropionic acid (ibuprofen) .

17-24

Authors: Cece Sumantri, Nurhayati D, Ahmad Farajallah, A Anggraeni

The aims of this study were to identify the effects polymorphism of β-lactoglobulin gene on milk yield and percentage of milk protein and fat in local sheep. A total number of 83 heads of lactating ewes raised under an extensive management at Jonggol Animal Science Teaching and Research Unit (JASTRU) of the Faculty of Anima Science, Bogor Agricultural University were studied. Research activities were carried out through some step involving blood collection. DNA isolation, DNA amplification and separation of DNA fragments by electrophoresis with silver staining method. By using polymerase chain reaction (P R) then genotyped by single strand conformation polymorphism technique (SSCP), it was success fully amplified a fragment length at 420 bp in β-lactoglobulin gene located on the exon 7. The electrophoresis pattern revealed 5 types of β-lactoglobulin gene and designated as AB. C, D and E types. The proportion for those respective 5 type from the highest to the lowest were for A (27.7%)C (16.87%), D (12.05%), (10.84%) and B (9.64%) respectively. There were no significant effect polymorphism β-lactoglobulin gene on both milk yield and quality in I cal sheep at JASTRU.

39-43

Authors: Maria Imelda, Aida Wulansari, Sumarnie Sumarnie

Pandanus conoideus Lam or 'Buah merah' of the Pandanaceae is native to East Indonesia, particularly Papua and North Maluku. Traditionally the fruits are used for health promotion and maintenance as well as for curing several illnesses. Recently, it has been reported that the fruits are potential for cancer medication . As a result, there has been an overexploitation of the plants from their habitats . In order to anticipate their possible disappearance due to overexploitation in the wild, an efficient and effective technology for the mass propagation, conservation and cultivation of these plants should be developed. Generally buah merah is propagated vegetatively by offshoots and stem cuttings or generatively by seeds. Micropropagation has many advantages over the conventional method. because the technique allows mass cIonal and pathogen-free production of plants at high rate of multiplication all year round . In this research the effects of 0,1 -0.2 mg/l thidiazuron (TDZ), 0.5- 1.0 benzyl amino purine (BAP) and 0.25-0.5 mg/l Kinetin (KN) on shoot bud induction and proliferation of P. conoideus were investigated using nodal sections or lateral buds or P. conoideus on modified Murashige and skoog medium. Shoots were rooted on MS medium without plant growth regulators (PGR) . The result showed that lateral buds of Pandanus started to initiate growth after 4-7 days in culture. The best medium for shoot proliferation was MS containing either 0,5 mg/l BAP with 0.1 mg/ l TDZ or I mg/l BAP with 0.5 mg/I KN. giving a multiplication rate of I6.5 shootlets per shootbud explant after 8 weeks Rooting of shoots was successfully conducted on MS medium without PGR. Acclimatization of rooted plantlets was achieved on a mixed medium of cocopeat and soil (1:1).