International Journal of Pharmaceutical Sciences and Drug Research

159-164

Authors: V. Vinoth Prabhu1*, N. Seraj Kumar1, V. Gopal2

The angiogenesis activity was involved in many physiological and pathological conditions. The research on angiogenesis activity was increased and many new methods are come for the evaluation of the angiogenesis activity. Previously Robert Auerbach et al., described the methods used for the evaluation of angiogenesis activity. We added some more techniques to that which are currently used for the evaluation of angiogenesis activity. Some of ischemic models are also used for the evaluation of angiogenesis like hindlimb ischemia model, coronary artery ligation model and Langendorff isolated model. Some of the new methods are introduced for the evaluation of inflammatory angiogenesis i.e. sponge granuloma angiogenesis assay. The CAM assay, corneal angiogenesis assay and matrigel plug assays are used for the evaluation of both angiogenic and anti-angiogenic agents. We also described some of the quantification techniques which are easily performed in laboratory like capillary density and estimation of hemoglobin in Matrigels.

176-181

Authors: M. Bhanumathy1*, S. B. Chandrasekar1, Uma Chandur2, T. Somasundaram1

Celastrus paniculatus (CP), a traditional Ayurvedic medicinal plant used for centuries as a memory enhancing, anti-inflammatory, analgesic, sedative and antiepileptic agent. The seed extract has been extensively investigated in several laboratories for their neuropharmacological effects and a number of reports are available confirming their nootropic action. In addition, researchers have evaluated the anti-inflammatory, anticonvulsant and other pharmacological effects of CP preparations/extracts. Therefore, in view of the important activities performed by this plant, investigation must be continued in the recently observed actions described in this paper. Moreover, clinical studies have to be encouraged, also to evidence any side effects and possible interactions between this herbal medicine and synthetic drugs.

188-192

Authors: Raghunadha Reddy Seelam1*, Sarath Chandiran I2, Koteswara Rao Divi2, Jayaveera K. N.3

A simple, sensitive and selective method for the quantification of Telmisartan by using rapid high-performance liquid chromatography/positive electroscopy ionization tandem mass spectroscopy. The method consists of extraction with Acetonitrile followed by the analysis of the Extracted sample by liquid chromatography-mass spectroscopy (LC-MS/MS) in multiple reaction monitoring mode using electrospray ionization mode (ESI). Chromatography was performed on a C18 reverse phase column, Methanol: Acetonitrile: 10mM ammonium acetate (45:45:10) as a mobile phase. The assay exhibited a linear dynamic range of 10 to 700ng/ml for Telmisartan in human plasma. Stability assessment was also included. A run time of 2.5 min for each sample made it possible to analyse healthy volunteers participating in pharmacokinetics drug-drug interaction studies.

199-203

Authors: S. T. Birhade*, V. H. Bankar, P. D. Gaikwad, S. P. Pawar

The present study was aimed to investigate the potential of cyclodextrin complexation as an approach for taste masking. For this purpose, Rizatriptan benzoate (RZBT) was selected as model drug which is having bitter taste. Taste improvement of drug by β-Cyclodextrin was done by simple complexation approach using physical and kneading mixture methods with various ratios. Taste perception study was carried out in-vitro by spectrophotometrically and in-vivo by gustatory sensation to evaluate the taste masking ability of binary complexation. The optimized taste masking ratio 1:10 of kneading mixture was selected based on bitterness score and characterized by fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC) and X-ray diffractometry (XRD) to identify the physicochemical interaction between drug and carrier and its effect on dissolution. In-vitro drug release studies for physical mixture and kneaded system were performed in pH 1.2 and 6.8 buffers. The FTIR, DSC and XRD studies indicated inclusion complexation in physical mixture and kneaded system. Both the binary systems showed effective taste masking and at the same time showed no limiting effect on the drug release. Whereas in comparison; kneading system showed better results. The results conclusively demonstrated effective taste masking by β-Cyclodextrin in both binary systems, which can be utilized as a novel alternative approach for effective taste masking.

210-212

Authors: R. K. Maheshwari1*, Sita Prasad1, P. Pandey2, G. Wanare1

The term hydrotropy has been used to designate the increase in solubility in water of various substances due to the presence of large amount of additives. Concentrated aqueous hydrotropic solutions of sodium benzoate, urea, nicotinamide, sodium salicylate, sodium ascorbate and sodium glycinate have been observed to enhance the aqueous solubility of many poorly water-soluble drugs. In the present investigation hydrotropic solubilization technique has been employed to solubilize the poorly water-soluble NSAID, piroxicam. Determination of solubilities of the drug in 1.5 M ibuprofen sodium hydrotropic solution and distilled water was carried out at 28±1°C. There was more than 50-fold enhancement in aqueous solubility of piroxicam with 1.5 M ibuprofen sodium (as compared to aqueous solubility). Therefore, it was thought worthwhile to solubilize the poorly water-soluble piroxicam from fine powder of its tablets to carryout spectrophotometric analysis at 358 nm. Ibuprofen sodium does not show any absorbance above 300 nm. Beerۥs law was obeyed in the concentration range of 5-35 μg/ml. Tablets containing piroxicam have been analyzed successfully. Recovery studies and statistical data proved the accuracy, reproducibility and the precision of the proposed method. Based on the same principle a large number of drugs having λmax above 300 nm can be estimated by 1.5 M ibuprofen sodium (inexpensive hydrotropic agent). Thus, hydrotropic solutions can be used in place of organic solvents (which are pollutants, toxic and give error due to volatility).

216-218

Authors: Umesh K. Singh1*, Surendra N. Pandeya2, Sandeep K. Sethia1, M. Pandey3, A. Singh1, Anuj Garg1, Pawan Kumar1

Substituted sulfonamides were reacted with different aromatic aldehydes to form Schiff’s bases. TLC ascertained the purity of synthesized compounds on silica gel G coated plates and visualized by using iodine vapour. The structures of synthesized compounds were confirmed by their IR, 1H NMR spectroscopic data. The derivatives were subjected to antimicrobial activity using different bacterial strains (Klebsiella pneumoniae, gram negative bacteria and Staphylococcus epidermidis, Bacillus subtilis as gram positive bacteria with respect to Ciprofloxacin as standard antibiotics.