International Journal of Pharmaceutical Sciences and Drug Research

6-8

Authors: Kaushik D.1*, Khokra S. L.1, Kaushik P.1, Saneja A.1, Sharma C.2, Aneja K.R.2, Chaudhary B.1, Koshy S.3

The present study was designed to evaluate the analgesic and antimicrobial activities of ethanolic extract of leaves of Mitragyna parvifolia plant (Rubiaceae) family. The analgesic activity was carried out on swiss albino male mice by Eddy’s hot plate and Acetic acid induced writhing test. The extract showed only moderate analgesic potential in acetic acid induced writhing test at all the test doses while the extract at the dose of 500 mg/kg (P<0.01) showed strong analgesic activity comparable to standard drug Diclofenac sodium (50 mg/Kg, i.p.) in hot plate method. The extract in different concentrations was also tested for antibacterial activity using agar well diffusion method. The extract significantly inhibited S. aureus and showed some degree of inhibition against P. aeruginosa and E. coli.

13-18

Authors: Sanjay Dey1, Bhaskar Mazumder1, J. R. Patel2*

The aim of this study was to investigate the effect of DMSO on the permeation of acyclovir in the form of topical gel formulations. Different formulations were prepared containing carbopol 934P, acyclovir (1 % w/w) and selected concentration of DMSO (0 to 10% w/w) to evaluate drug content, spreadibility, pH, viscosity, and in-vitro permeation through mouse epidermis and porcine skin. FTIR spectrometry was used to investigate physical state of drug in the gel formulations. The mechanisms of drug permeation were evaluated by FTIR spectrophotometer and histopathological studies. The carbopol 934P gel was found to contain 95.62 to 98.89 % of acyclovir and spreadibility was found in the range of 10.75 to 11.75 g.cm/sec. The pH of all formulations was found near to the skin pH value. The viscosity of the formulations was found inversely proportional with drug permeation. A maximum permeation flux of acyclovir (463.42±36.41µg/cm2.h) through porcine skin was observed with an enhancement ratio of 1.55, when DMSO was incorporated at a concentration of 10%w/w in gel system. The FTIR spectra revealed the absence of drug-polymer interaction. From FTIR spectroscopy and histopathological studies it was evident that the permeation of acyclovir, across mouse and porcine skin, were increased in presence of DMSO which can be attributed to the partial extraction of lipids in the stratum corneum. The results suggest that DMSO may be useful for enhancing the skin permeability of acyclovir from transdermal therapeutic system containing carbopol 934P gel as reservoir.

24-27

Authors: Nikhil K. Sachan1*, Yatindra Kumar2, Seema Pushkar1, R. N. Thakur2, Sudhir S. Gangwar2, V. K. Kalaichelvan3

The present investigation aims to examine the diabetic potential of the plant Ficus racemosa in normal and alloxan induced diabetic rats. The bark extract with water, petroleum ether and with alcohol were screened for blood glucose lowering activity and the alcoholic extract having better therapeutic potential was prepared through Soxhlet extraction for further study. Alcoholic and aqueous extract of bark of Ficus racemosa at a dose of 400 mg/Kg was given to normal and alloxan induced diabetic rats and the blood samples taken from the retero-orbital plexus vein were analyzed for blood glucose level as per standard protocol with available kits through Auto-analyzer. The comparison of blood sugar level as per model schedule showed that in normal group the ethanolic extract, at a dose of 400 mg/Kg intra-peritoneal, the blood glucose lowering 28.66 % while in aqueous extract given group it was 25.90 %. In alloxan induced diabetic rats decrease in blood glucose level in aqueous and ethanolic extract group was found to be 27.01 % and 45.03 % respectively. In conclusion, the ethanolic extract of Ficus racemosa reflected anti-diabetic potential through its glucose lowering activity in experimental animals. It supported the folklore claim of anti-diabetic activity of the plant.

32-35

Authors: Jani Rupal1*, Jani Kaushal2, Setty C. Mallikarjuna.3, Patel Dipti1

Aceclofenac is a potent anti-inflammatory analgesic agent indicated for acute and chronic treatment of rheumatoid arthritis, ostio arthritis, and onkylosing spondylytis. Aceclofenac is poorly water soluble and may show dissolution limited absorption. Solid dispersions (SDs) of Aceclofenac in PEG-6000, PVP were prepared by solvent evaporation method. The solid dispersion was characterized for physical appearance, solubility and IR. FTIR study reveled that drug was stable in SDs. Solubility of Aceclofenac from SDs increased in distilled water. The drug content was found to be high and uniformly distributed in the all formulation. Dissolution of drug increased from all the solid dispersion. Dissolution of Aceclofenac increased with increase in the proportion of carriers (1:1, 1:5, and 1:9). Of both the carriers used, dissolution of the drug was more in PEG 6000 based SDs. It is concluded that dissolution of the Aceclofenac could be improved by solid dispersion and PEG 6000 based solid dispersions were more effective in the enhancing the dissolution.

43-45

Authors: Choudhary B.1*, Goyal A.1, Khokra S. L.2, Kaushik D.2

A simple, accurate and reproducible HPLC method have been developed for simultaneous estimation of Diclofenac sodium and rabeprazole from their tablets formulations. A phenomenex C18 (Luna) column of length 250×7.5 mm with particle size of the stationary phase 5 µm and S mobile phase potassium dihydrogen phosphate buffer (pH adjusted to 7.5 with 1 M sodium hydroxide) and acetonitrile in the ratio 60: 40 were used in this study. Retention time was found to be 9.20 min and 6.40 min for Rabeprazole and diclofenac sodium respectively. While that for internal standard as domperidone was 11.87 min at a flow rate of 2ml / min. Linearity was found in the concentration range of 10-50 µg /ml for both the drugs in this method. The results of analysis have been validated statistically and also by recovery studies.

51-54

Authors: Rai V. K.1*, Pathak N.1, Bhaskar R.2, Nandi B. C.1, Dey S.1, Tyagi L. K.3

The purpose of this research is to prepare Raloxifene Hydrochloride immediate release tablet by wet granulation technique. In order to obtain the best, optimized product six different formulations were developed. Different filler, binder, disintegrant and lubricant were taken as variables. Weight variation, thickness, hardness, friability, disintegration time, in-vitro release and pharmaceutical assay were studied as response variables. Sticking was observed when the formulation containing stearic acid and sodium stearyl fumarate. However, in the remaining four formulation containing magnesium stearate, no sticking was observed. The formulation NP061 was selected as an optimized product. The different physical properties and in-vitro release profile showed best comparable with reference product. Optimization has proven as an effective tool in product development.