38-45
Orostachys japonicus ethyl acetate fraction suppresses MRSA biofilm formation
Authors: Jae-Hyeon Kim, Su-Yeon Han, Ji-Hye Kwon, Dong-Seok Lee
Number of views: 173
Objective: To investigate the effect of Orostachys (O.) japonicus, a
perennial herbaceous plant of the Family Crassulaceae, on biofilm
formed by methicillin-resistant Staphylococcus aureus (MRSA).
Methods: Powdered O. japonicus was extracted by 95% methanol,
concentrated, and then, systematically fractionated with n-hexane,
dichloromethane (DCM), ethyl acetate (EtOAc), n-butanol, and
H2O according to polarity. Among them, the flavonoid-rich EtOAc
fraction demonstrated the highest antibacterial activity and was
used in this study. Using the biofilm inhibition assay, cell-surface
attachment assay, confocal laser scanning microscopy, latex
agglutination assay, and real time qRT-PCR, we examined whether
the EtOAc fraction inhibited the formation of MRSA biofilm.
Results: The EtOAc fraction exhibited distinct activity against
biofilm formation and cell-surface attachment of MRSA up to 1
mg/mL through down-regulating the expression of mecA gene and
the production and agglutination of penicillin-binding protein 2a as
solidly observed in biofilm inhibition assay, cell-suface attachment
assay, confocal laser scanning microscopy, latex agglutination assay,
and real time qRT-PCR analysis.
Conclusions: These results suggest that O. japonicus could be
utilized as a potential resource for the development of new antibiofilm
formation of MRSA and antibacterial agents in the future.