Lipopolysaccharide was extracted from Campylobacter jejuni and Vibrio cholerae (NAG) by EDTA-heating method. Anti-sera against both LPS were raised in rabbit. Passive hemagglutination was used to check the LPS-antigenic relationship between both bacterial species. The results showed that there is no cross reaction relationship between LPS of C. jejuni and LPS of V. cholerae.
Three hundred and sixty feces samples were collected from children and infants who have diarrhea and other symptoms that associate with Acute Flaccin Paralysis (AFP). The samples were collected from middle and southern provinces of Iraq. The clinical cases were equal or less than four years old. Nineteen positive poliovirus positive cases were found post preliminary culture. Poliovirus type I was found in six cases, Poliovirus type II was found in three cases and Poliovirus type III was found in six cases. Four cases containing mix poliovirus types. All positive cases were screened in confirmation test and the results showed that 9 isolates of type I, 3 isolates of type II and 10 isolates of type III. All isolated viruses were sabin type and no wild type poliovirus was identification in current study. It can be concluded that the poliovirus sabin maybe associated with Acute Flaccin Paralysis.
This study included nine patients with inactive carrier state of HBV and 14 healthy control groups. The
number and the percentage of T- Lymphocyte (CD3+ Cells) in the peripheral blood of these groups
showed no significant difference. Similar trend was observed when number and percentages of T
helper cells (CD4+ cells) and T cytotoxic lymphocytes (CD8+ cells). Moreover, no significant
difference in CD4+ /CD8+ cells ratio (P > 0.05) in peripheral blood of patients with inactive carrier
state of HBV as compared with healthy control group. The levels of total serum bilirubin (TSB)
concentration and alanine aminotransferase (ALT) activity were similar to control group. The levels of
immunoglobulin concentration (IgG and IgM) in patients group were similar to control group. No
remarks of autoimmune phenomena were observed in patients group.
The chitinase is an enzyme has a good ability to hydrolyze chitin in nature. Thus this enzyme play an important role in clean the environments and in industries. The activity of the free chitinase to catalyze the chitin was evaluated in previous study. The activity of immobilized chitinase on a charcoal was not studied previously. In current study, the activity immobilized chitinase to hydrolyze chitin was studies. Purified chitinase was immobilized on charcoal then the activity of immobilized was checked and compared with activity of free chitin. The results showed that the activity of immobilized chitinase was significantly higher than the activity of free chitinase. The activity of immobilized chitinase was checked at different physical conditions like pH and temperature. Maximum enzyme activity to hydrolyze chitin was observed at pH 8 and temperature 40 OC. The present study showed that the activity of chitinase increased when the enzyme was immobilized on charcoal.
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