Advancement in Medicinal Plant Research

Number 3, 2021

48-58
Determining the effective compounds of Salvia miltiorrhiza through chromatographic fingerprinting coupled with chemometrics

Authors: Yuan-Kai Tu, Jui-Sheng Lai, Bo-Jein Kuo, Ruo-Han Jian and Tsing-Fen Ho

Abstract

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Herbs are rich in the active ingredients of drugs for preventing or treating various disorders. However, conventional bioactivity-guided separation is time and labor-intensive and neglects the additive effect of multiple components. These problems hinder the development of new medicines from natural products. This study established a chemometric analysis method that integrates processes based on the spectrum-effect relationship for the rapid identification of the primary active components of a plant. The high-performance liquid chromatography (HPLC) fingerprints of 171 Salvia miltiorrhiza extracts (SMEs) with varied constituent profiles were analyzed. Chemometric analysis was performed to establish an HPLC fingerprint–bioactivity relationship to explore the components of SMEs that contribute to the antioxidant activity and cytotoxicity effect, respectively. The results indicated that the developed strategy can be used to identify components largely contributing to particular bioactivities and re-evaluate the efficacy of previously neglected components. The present study identified not only the primary active components of S. miltiorrhiza but also the optimal ratios of constituents, validating the method for use in the future investigation and development of herbal medicines.

59-67
Evaluating efficacy of Punica granatum L. peel extract gel in treating Cyclophosphamide induced alopecia in mice and in vitro anti-pediculicidal activity

Authors: K. Ravishankar, G. V. N. Kiranmayi and P. Devakusuma

Abstract

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Number of views: 88

The present study aimed to estimate the efficacy of Punica granatum L. ethanolic peel extract gel in treating hair fall in mice using the cyclophosphamide-induced Alopecia method. In vitro anti-pediculicidal activity of peel extract was evaluated using the Filter paper diffusion method. Results indicated that high dose 20%w/v ethanolic peel extract gel of P. granatum was observed to have efficacy in treating Alopecia and the results compared with that of standard minoxidil and it is also indicated that there is significant Anti pediculicidal Activity to 20%w/v ethanolic peel extract was observed and results are compared with that of standard Benzyl Benzoate 25%w/v. These results provided an important basis for developing the peel extract as novel anti-pediculicidal activity and for treating Alopecia and promoting hair growth, Animals treated with High dose 20% gel showed better results compared to 10% low dose gel.