In vitrosensitivity methods against various antifungals were performed for otomycotic agents isolated from 10 patients. The agents were isolated on SDA supplemented with chloramphenicol @ 30μg/100ml. The common fungi isolated were Aspergillus fumigatus, A. flavus, A. terreus, A.alternata, Paecilomyces, andCandida albicans. These clinical isolates were subjected to in vitro sensitivity methods against synthetic antifungals and natural herbs and spices. The in vitro sensitivity methods applied were disc diffusion method, E test and broth dilution method. The most effective antifungal was itraconazole and garlic was the most effective amongst the natural products tested.
This research work was carried out to investigatethe antimicrobial activities of isolated fractionof Bixa orellanaand Linum usitatissimumagainst five bacterial and five fungal pathogen.Leaves Powder of bixa orellanaand seed powder of Linum usitatissimumsubjected to soxhletextraction. The separation of various fraction was carried out by column chromatography, analyzed and purified by thin layer chromatography(TLC) until homogeneity of the fraction achieved. The homogeneity and the purity of the different fractions were checked by HPLC.Antimicrobial evaluationof isolated fractions was carried out. Two fraction Fr-1 (352mg) and Fr-2(260mg) were collected from Bixa orellana had Rf was o.99 and 0.35. fraction 3 and 4 was isolated fromlinum usitatissimum with Rf 0.45 and 0.25.When compare to the standarddrug gentamycin,phenolic fraction of 1 and 2 had good antibacterial activity against all bacterial strain where as glycoside fraction 3 and 4 were poorest activity against all bacterial strain.Whencompare to standard drug tioconazolefraction-1 and 2 had very good activity against fungal strain and fraction 3 and 4 had moderate activity against fungal strain.
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