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Investigation of stingray spines by Fourier transform infrared spectroscopy analysis to recognize functional groups
Authors: Muthuramalingam Uthaya Siva, Mohideen Abdul Badhul Haq, Deivasigamani Selvam, Ganesan Dinesh Babu, Rathinam Bakyaraj
Number of views: 467
Objective: To investigate functional groups of toxic spines in stingray by Fourier transform
infrared spectroscopic analysis.
Methods: The venom extract of Himantura gerrardi, Himantura imbricata and Pastinachus
sephen were centrifuged at 6 000 r/min for 10 min. The supernatant was collected and preserved
separately in methanol, ethanol, chloroform, acetone (1:2) and then soaked in the mentioned
solvents for 48 h. Then extracts were filtered and used for Fourier transform infrared spectroscopic
analysis.
Results: The results identified that the presence of free amino acids and protein having β-sheet
and random coiled secondary structure. The presence of O-H stretch, C=O stretch, C-H stretch,
N-H deformation, O-H deformation and C-O stretch in the sample aligned with standard bovine
serum albumin. The influence of functional groups within the molecule was because of the impact
of preferred spatial orientation, chemical and physical interaction on the molecule. In conclusion,
compared to bovine serum albumin, Himantura imbricata consists of two C=O stretch, are
involved in the hydrogen bonding that takes place between the different elements of secondary
structure.
Conclusions: The venom of poisonous animals has been extensively studied, since standard
medicine not available for treatment against injuries causing stingray. Therefore, it's the baseline
study, to motivate further process and produce effective antibiotics.