Buletin Kebun Raya

40-46

Authors: Yupi Isnaini, Elizabeth Handini

Mature seeds of a pitcher plant (Nepenthes grocilis Korth.) were cultured in seven different media, namely half strength MS medium, half strength MS medium supplemented with BAP and NAA, 0.8 KC medium supplemented with coconut water + bean sprout extract, 0.8 KC + H3B03 + CuSO3 + ZnSO4 + coconut water + bean sprout extract, 0.25 KC + Vitamin (MS), HYPONeX + coconut water + sprout bean extract, and HYPONeX + potato extract. Each culture was incubated under light conditions. Young seedlings were subsequently transferred onto fresh media of 0.5 MS and 0.25 KC supplemented with 0, 5, 10, and 15 mg/I GA3 after 15 weeks of sowing. Seed germination and seedling growth were observed weekly. It was noted that the seeds started to germinate in ten weeks after incubation and the highest seed germination was obtained in HYPONeX medium + potato extract. However, the appearance of the seedlings was better on MS media and KC media without organic compound than on the other media. Seedlings in all level of GA3 in half strength MS media and 0.25 KC media grew faster than those in media without GA3.

53-59

Authors: Maria Utami Dewi Wardhani, Dwi Murti Puspitaningtyas, Diny Dinarti

A research was carried out in Orchid Laboratory of Bogor Botanical Gardens to observe the effect of gamma ray irradiation on the growth variation of Brachypezo indusiata (Reichb.f) Garray and suitable culture medium for propagating the plant. The research was arranged in a factorial experiment with a Completely Randomized Design. The first factor was gamma ray irradiation on protocorm-like­bodies (plb) of germinated seeds of B. indusiata in which four levels of irradiation was applied, i.e. 0, 10, 20, and 30 Gy. The second factor was the use of two different culture media, i.e. Vacin & Went medium supplemented with either banana pulp or a mixture of tomato and sprout bean as the essential organic compound of the medium. The result showed that the irradiation treatment improved the survival of B. indusiata explants, most significantly at 20 Gy level of irradiation, and could modify the growth of the resulted plantlets by reducing the height of the plant and the length and width of the leaves. The higher the level of irradiation the smaller the plantlets produced. Meanwhile, the addition of organic compound derived from banana fruit into the basal medium of Vacin & Went enhanced the growth of the plantlets better than that derived from a mixture of tomato fruit and bean sprout. However, there was no significant interaction effect detected between the irradiation treatments and the use of different culture media.

67-70

Authors: N Lugrayasa, N K Erosi Undaharta

Gunung Ranaka merupakan suatu kawasan hutan konservasi yang terletak di Desa Ranaka, Kecamatan Wairii, Kabupaten Manggarai, Nusa Tenggara Timur. Di kawasan ini terdapat Taman Wisata Alam (TWA) Ruteng yang dikenal sebagai pusat persebaran flora di Gunung Ranaka. Berdasarkan Surat Keputusan Menteri Kehutan-an Nomor 456/Kpts-11/1993 kawasan konservasi ini mencakup areal seluas 32.245,60 ha atau sekitar 7,69% dari luas Kabupaten Manggarai. Secara keseluruhan topografi kawasan TWA Ruteng berupa deretan pegunungan yang dikenal sebagai jajaran Pegunungan Ruteng dengan beberapa puncaknya antara lain Poco Mandosawu (2.3W m dpl), Poco Nembu (2.030 m 01), Poco Ranaka (2.140 m dpl), Golo Lede (1.990 m dpl), Poco Nao (1.920 m dpl), Gob Curunumbeng (1.800 m del), dan Gob Ranamese (1.790 m dpl). Hampir dua per tiga dad lereng-lereng pegunungan tersebut mempunyai kecuraman lebih dari 40% wau derajad kemiringan sekitar 22°.