Journal of Science and Applications: Biomedicine

Number 4(1), 2016

1-7
Vitamin A metabolite inhibits proliferation, migration and osteogenic differentiation in a human periodontal ligament stem cells subpopulation

Authors: Lin Jiang 1,3, Xuesong Yu 3, Guo Ye 3, Wenping Luo 2,3, Yan Zhang 1,3 *

Abstract

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The periodontal ligament stem cells (PDLSCs) are regarded as the promising seed cells for in situ facilitated endogenous periodontal tissue regeneration. Nevertheless the patients with hypervitaminosis A and alcoholism become very difficult to repair periodontal defects and guide bone regeneration. The purpose of this study was to evaluate the effect of all-trans retinoic acid (ATRA) on the recruitment and osteogenic differentiation in human PDLSCs. The periodontal ligament tissues were obtained from healthy premolars extracted for orthodontic reasons and used to isolate and characterize the hPDLSCs subpopulation. Following various concentrations of ATRA treatment, cell proliferation and migration of hPDLSCs were significantly inhibited, and the cell cycle progression was significantly arrested with the high-dose of ATRA group. Furthermore, with the increase of ATRA concentration, both the early and late stages of osteogenic differentiation were suppressed in hPDLSCs. Our results

8-14
Giant hydronephrosis in children: resection or preservation, what is the best approach?

Authors: Li Xue, Tie Chong, Qi Chen, Hecheng Li, Peng Zhang, Zhenlong Wang, Ziming Wang*

Abstract

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Number of views: 188

The purpose of this study is to assess functional and morphologic recovery in children after dismembered pyeloplasty for GH caused by UPJO of children. From Jan 2005 to Dec 2010, 38 pediatric patients with GH who underwent dismembered pyeloplasty performed by a single surgeon were retrospectively analyzed. Patient follow-up was at least 3 years. The outcomes of different types of UPJO were compared. DRF and RPT assessed more than 3 years postoperatively were compared according to ages and time. Improvement of DRF and RPT was also compared. The relationship between the improvement in DRF in the first six months and renal function assessed in 3-year was also analyzed. The results showed that the renal function of functional obstruction was significantly lower than other groups (p＜0.0001). GH patients who underwent pyeloplasty before 36 months of age (n = 12) experienced more improvement in DRF compared with GH patients who underwent surgery after 36 months of age (n = 21) (p＜0.0001). DRF measured in the first six postoperative months was significantly higher than other groups (n = 12) (p＜0.0001). There is a positive positive correlation between DRF in the first six months and renal function assessed in 3-y (r =0.8193). In conclusion, it is worthwhile to preserve the kidney in children with GH. Functional obstruction should be independent from UPJO and surgical treatment is inadvisable. The rate of improvement in DRF in the first six months is a good indicator to outcome.

15-16
Curative cord blood hematopoietic stem cell transplantation (CBT) for HIV infection and thalassemia major (Thal)

Authors: Michelle Chow 1, Vincent Guo 2, Elizabeth Rao 2, Tony Zeng 2, Issac Hsu 2, Christine Chow 1, Brian Wang 1,2, Lawrence Petz 3, Tung-Her Jaing 4, John Chow 7,8, and Robert Chow 5,6,7,8*

Abstract

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Number of views: 188

Unrelated donor CBT (UCBT) is a proven therapy, saving thousands of lives yearly. Of UCBT’s many curative indications, Thal and HIV infections have unique considerations.