Journal of Science and Applications: Biomedicine

34-40

Authors: Jintong Li 1#, Xiao Liang 1#, Juan’e Dong 1*, Guowei Wang 1, Zongsuo Liang 1, 2*

Salvia miltiorrhiza Bunge is a commonly used traditional Chinese medicine whose water-soluble components are the key active ingredients for its therapeutical effects. In order to illustrate the synthesis, translocation, and distribution of the water-soluble phenolic compounds in the different growing stages of S. miltiorrhiza Bunge, levels of salvianolic acid B (SAB) and rosmarinic acid (RA) were examined we performed histochemistry and high performance liquid chromatography (HPLC) to quantify the amounts. We found that the main phenolic compounds synthesized initially during the sprouting stage to be SAB and RA, which are both mainly located in the central vacuoles of palisade parenchyma and epidermis cell in the leaf during this particular stage. Of the phenolic compounds initially located in the plasma membranes of periderm and phloem parenchyma cells during the germination stage of the root, there is a large amount of RA yet little presence of SAB. Located in the central vacuoles of the periderm cell of the root after leaf wilting are the main components, SAB and RA.

45-55

Authors: Guoming Su 1#, Jun Yang 2#, Liren Hu 3, Zhuqing Fu 4, Zuguo Zhao 4, Weiqing Yang 1*

We aimed to investigate the correlation between the four common single nucleotide polymorphisms (SNPs) in microRNAs (miR-146a rs2910164, miR-196a2 rs11614913, miR-499 rs3746444 and miR-149 rs2292832) and hepatitis B virus-related hepatocellular carcinoma (HBV-related HCC) risk through a systematic review and meta-analysis. Odds ratios (ORs) along with 95% confidence intervals (95% CIs) were pooled to explore the strength of associations between SNPs in microRNAs and HBV-related HCC risk. All statistical analyses were performed using Stata 12.0 software. For miR-146a rs2910164, a significantly decreased risk of HBV-related HCC development was observed under recessive model (CC versus GC+GG: OR = 0.83, 95% CI 0.71-0.98, P = 0.03), and results remain robust in Asian populations. As for miR-196a2 rs11614913, significant association was found under four genetic models (T versus C: OR = 0.61, 95% CI 0.43-0.87, P = 0.01; TT versus CC: OR = 0.35, 95% CI 0.16-0.75, P = 0.01; TT+CT versus CC: OR = 0.59, 95% CI 0.36-0.96, P = 0.03; TT versus CT+CC: OR = 0.42, 95% CI 0.21-0.87, P = 0.02) among Caucasians, but no significant association on Asians. The current meta-analysis demonstrates that miR-146a rs2910164 polymorphism may play a potential role in genetic susceptibility to HBV-related HCC in Asian populations.