International Invention Journal of Medicine and Medical Sciences

Number Vol. 3(8), 2016

165-168
Antimicrobial Property of Crude Ethanolic Extract of Mucuna pruriens leaves on Pseudomonas aeruginosa, Salmonella typhi and Shigella dysentriae

Authors: *Abraham, O.J.1, Nwobodo, A.H.2, Ngwu, B.A.F.3, Onwuatuegwu, J. T.C.4, Egbunu, Z.K.1, Yahaya, O.1, Amodu, A.E.1, Onuh, I.5 and Salihu, A. M.1.

Abstract

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Mucuna pruriens also called velvet bean belongs to the family fabaceae. It is used in traditional medicine to cure worms infestation, dysentery, diarrhoea, snake bite, sexual debility, cough, tuberculosis and diabetes. This research was carried out to determine the antimicrobial activity of crude ethanolic extract of Mucuna pruriens leaves on Pseudomonas aeruginosa, Salmonella typhi, and Shigella dysenteriae. Agar well diffusion method was used to study the effect of the plant on the organisms at 300mg/ml, 400mg/ml and 500mg/ml. The Minimum Inhibitory Concentration (MIC) on each organism was 300mg/ml. At the concentration of 300mg/ml the mean zone of inhibition was 10mm, 13mm, and 13mm for Pseudomonas aeruginosa, Salmonella typhi, and Shigella dysenteriae respectively. At 400mg/ml, the mean ZI were 11mm, 18mm and 14 respectively, while at 500mg/ml, the mean ZI were 15mm, 20mm and 20mm on the organisms respectively. The zone of inhibition increased with concentration used. The result obtained showed that this plant could be further studied as alternative means to cure the ailment caused by the aforementioned microorganisms. Therefore, further studies have to be done using other solvents extraction.

173-178
The use of TERT and CK20 as a trial for early diagnosis of HCC in animal model

Authors: Omnia Awad Ibrahim1, Mahmoud Mohamed Zakaria2*, Mahmoud Gamil Elsebaei3, Khaled Khilo4

Abstract

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To evaluate the use of TERT and CK20 gene expression as a diagnosis of HCC in animal model. Thirty adult albino male rats were divided into 3 groups of 10 animals each. Animals in group A underwent a sham experiment, group B was injected by CCL4 for 4 weeks (early) and group C was injected by CCL4 for 8 weeks (late). At end of experiments, specimens from liver were all stained by H and E dye. TERT and CK20 gene expression were done by Real-Time PCR in blood and tissue samples. The relative quantification of target genes and statistical analysis were done. A histopathological study of the rat liver of early group showed mild fibrosis and the late group showed formation of cirrhotic nodule with dysplasia of hepatocytes. TERT mRNA was increased significantly in early and late tissue by about 17 and 23 folds and in blood samples, the expression was increased about 3 and 11 folds, respectively. The gene expression of CK20 was not observed in blood stream but was expressed significantly in tissues with about 4 and 44 folds. P value was <0.001 between control and early or late HCC. Human TERT gene expression was observed to increase according to HCC in both tissue and blood samples but CK20 was regulated in tissue samples.