In Vitro Antimicrobial Activity and Cytotoxicity of Maerua oblongifolia
Authors: Ehssan. H. O. Moglad, Omer. M. Abdalla, Haider Abd Algadir, W. S. Koko and A. M. Saadabi
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The research was carried out to search new antimicrobial agents mainly from plant extracts with the goal to discover new chemical structures by investigate in-vitro antibacterial and antifungal activity of Maerua oblongifolia. Eighty percent Methanol and chloroform extracts of leaves and stems were screened for their antimicrobial activity against different pathogenic bacteria and fungi. These were Staphylococcus aureus, Bacillus subtilus, Escherichia coli, Salmonella typhi, and Aspergillus niger and Candida albicans using the cup plate agar diffusion method. All extracts exhibited inhibitory effects against most of the tested organisms with zones of inhibition ranging from 13-20 mm. The largest inhibition zones were obtained from methanol stem extract of Maerua oblongifolia against Aspergillus niger and chloroform stem extract against Escherichia coli. In vitro cytotoxicity assays were performed using MTT assay and used 3T3 NIH mouse embryo fibroblast cell line, and CC-1, a rat Wistar hepatocyte cell line. The results obtained indicate that this plant has nontoxic effect on 3T3 with less than 20% of growth inhibition and has nutritional value on CC-1. Therefore, this finding indicated that these extracts of such plant promising antimicrobial agents.
Keywords: Antimicrobial activity, Medicinal plants, Maerua oblongifolia, Cytotoxicity, Sudan.