Indonesian Journal of Animal and Veterinary Sciences

253 - 261

Authors: Y. Widiawati

The aim of this study was to evaluate the effect of pineapple waste substitution to mixed basal diet of Elephant grass and calliandra on rumen ecosystem. Pineapple waste was substituted to basal of Elephant grass and calliandra leaves (3:2) at the level of 0% (RA); 10% (RB); 20% (RC); 30% (RD); 40% (RE) and 50% (RF). In this experiment 24 Indonesian local male sheep (9-10 months old, 15.3 kg average body weight) were used, and were divided into 6 groups of dietary treatment (4 sheep each). Every group was offered one of the experimental diets (RA to RF) in a Completely Randomized Design. Pineapple waste was offered gradually for one month until the level of 50% (RF) was reached. The animals were adapted to experimental diets for about 14 days prior to the data collection period. Rumen fluids from each animal was taken (5 hours after morning feeding) for pH, ammonia concentration; bacteria and protozoa population analysis. The results showed that substitution of pineapple waste up to 30% had no effect on pH, but when the level was increased up to 40 and 50%, the pH (P<0.01) decreased. Ammonia concentration was similar when 10% of the pineapple waste was included, then it decreased significantly when the waste was given up to 50% (P<0.01). A decrease in bacteria population and an increase in protozoa population happened when the waste given was increased up to 50% but it wasn’t significant (P>0.05). Increasing pineapple waste given increased population of amyllolytic bacteria but decreased the population of cellulolytic bacteria. On the Elephant grass and calliandra basal diet with the proportion of 3 : 2, the best substitution of pineapple waste was up to 20%. Key words: Pineapple Waste, Rumen, Bacteria, Protozoa, Ammonia

270 - 277

Authors: Antonius

The objective of this research was to evaluate the use of fermented rice straw by probion on feed consumption, digestibility, daily gain and feed efficiency of Simmental cow. This study was carried out based on completely randomized design, with here dietary treatments and four replications for each treatment. The treatments were R1 (JP-15) = 40% elephant grass + 15% untreated rice straw + 45% concentrate; R2 (JPF-15) = 40% elephant grass + 15% fermented rice straw + 45% concentrate; and R3 (JPF-35) = 20% elephant grass + 35% fermented rice straw + 45% concentrate. Concentrate was given at around 08:00 while unfermented/fermented rice straw was given afterward at around 09:00. Chopped elephant grass was given twice a day at 11:00 and 16:00. Water was available through out the day. Observation was done for two months on feed consumption, digestibility, daily gain and feed efficiency. The results did not show significant differences on feed consumption, digestibility, daily gain and feed efficiency, except on digestibilities of cellulose and hemicelulose. The digestibilities of cellulose and hemicelulose of treatment R3 was higher than that of R1 and R2. It is concluded that fermented rice straw is suggested to be used as an alternative feed to substitute elephant grass in maintaining feed consumption, digestibility, daily gain and feed efficiency of Simmental cow. Key words: Probion, Cow, Rice Straw

284 - 287

Authors: Afnur Imsya, Rizki Paluli

This research was conducted to study the effect of substrat levels and incubation time on changes of mean of: lignin, neutral detergent fiber and acid detergent fiber content of palm frond. This research was done based on completely randomized design with 2 factors as treatments. The first factor was substrate levels ie: 5, 10 and 15 litters, the second factor was incubation times: 3, 5 and 7 days of incubation which resulted in reduction content of lignin. Result of this research showed that treatments gave significantly different influence (P<0.05) on lignin, neutral detergent fiber and acid detergent fiber of palm frond. The best treatment was 15 litter of substrate with 7 days incubation, resulted in: 9.22 % lignin, 38.56% neutral detergent fiber, and 32.19% acid detergent fiber of palm frond. It is concluded that substrate level and incubation time interaction in biodegumming process decreased the level of lignin, NDF and ADF in palm frond. Key words: Biodegumming, Lignin, Neutral Detergent Fiber, Acid Detergent Fiber

295 - 306

Authors: Ismeth Inounu, Erfan ., R.H. Mulyono

It is important to know body measurement because it could be used to estimate body weight as well as to differentiate the chrateristic of body measurement and shape of animal due to different breed or environment. This research was carried out to study morphometric characteristic of body size and body shape from 78 of Garut sheep (GG), 29 HG sheep {crossbred between St. Croix (HH) and Garut sheep (GG)}, 36 MG sheep {crossbred between Mouton Charollais (MM) and Garut sheep (GG)}, 62 MHG sheep (MG x HG) and 38 HMG sheep (HG x MG). Body part measured were wither height (X1), rump height (x2), body length (X3), chest width (X4), chest depth (X5), hip width (X6), chest girth (X7), cannon circumference (X8) and hip length (X9). Data obeserved were analised using t test and Principle Components Analysis (PCA). Based on PCA it was showen that chest girth was the primary identity for body measurement on males and females of Garut, HG, MG, MHG and HMG with its Eigenvector value 0.689; 0.709; 0.689 and 0.681 respectively. The primary indentity for body shape of Garut sheep were chest girth and hip heigth with Eigenvector value -0.600 and 0.551 respectively. The primary indentity for body shape of HG sheep were body length with Eigenvector value -0.725. The primary indentity for body shape of MG sheep were chest girth, rump heigth, and wither heigth with Eigenvectors value: -0.600; 0.558 and 0.555 respectively. The primary indentity for body shape of MHG sheep was wither height with Eigenvector value 0.608. The primary indentity for body shape of HMG was body length with Eigenvector value 0.764. Body shape of HG and MG sheep is different than that of Garut sheep, but the body shape of MHG and HMG were close to Garut body shape. This result indicated that the adaptability to environment of HMG and MHG is close to that of Garut sheep. Key words: Sheep, Body Size, Body Shape

167 - 176

Authors: Tiurma Pasaribu, A.P. Sinurat, T. Purwadaria, P.P. Ketaren

Solid heavy phase (SHP), a by product material of palm oil factory obtained by ceramic filtration from liquid waste could be used as a feedstuff to replace corn in poultry diet. A series of experiment was carried out to improve nutrient value of the SHP by supplementation of enzymes and amino acids in order to increase the proportion of SHP to substitute corn in layer diet. There are three enzymes i.e.: Balitnak production (BS4), a commercial single enzyme (consist of mannanase) and comercial multienzymes were tested. All the enzymes were mixed with fresh SHP in different dose, dried and ground. The nutrient digestibility of these materials was measured in order to decide the optimum level of each enzyme. Based on this result, a feeding trial was carried out. Experimental diets were formulated to study the effect of substitusion of 25% or 50% corn with dried SHP or enzymes-treated SHP on the performances of the layers. The effect of methionine and lysine supplementation into diets contained high levels of SHP was also studied. Results showed that all enzymes studied could increase the energy (TME) of the SHP. BS4 enzyme and the commercial multienzimes, except single enzyme, also increase the true protein digestibility of the SHP. The optimum dose of each enzyme for each kg dry mater of SHP was 13.3 ml BS4, 2 g single enzyme and 3 g multienzymes. Substitution of 25% corn in layer diet with dried SHP or enzymes-treated SHP did not significantly impair the performances (hen-day egg production and FCR) of layers. However, substitution of 50% corn with SHP + multienzymes or SHP + single enzyme significantly impaired the performances of the layers. Addition of methionine and lysine amino acids restored the performance of the hens fed with SHP + commercial multienzyimes, but not those fed with high levels of SHP + commercial single enzyme. Substitution of 50% corn with SHP + BS4 enzime did not significantly impaire the performance of layers and therefore, addition of amino acids into the diet was not required. Substitution of 25% or 50% corn with dried SHP or enzymes-treated SHP did not affect the egg quality (HU, yolk color index and shell thickness). It is concluded that solid heavy phase (SHP) could be used to substitute 25 to 50% corn in layer diet, especially when the SHP was treated with BS4 enzyme. Key Words: Solid Heavy Phase, Enzymes, Amino Acids, Layer

192 - 199

Authors: Marthen L. Mullik, Bambang Permana

This grazing experiment aimed at assessing effects of providing high quality forage as supplements for Bali cattle grazing native pasture in the wet season. Twenty four Bali heifers with a mean live weight of 85.4±3.8 kg were allotted into four reatments. The treatments were (1) grazing only, (2) grazing + Gliricidia sepium leaf, (3) grazing + Acacia villosa leaf, and (4) grazing + Lannea grandis leaf. The heifers grazed together on a 25 ha Bothriochloa timorensis dominant pasture during the day (0700 h to 1700 h) in wet season, and supplements were provided at night. The supplements were given in accordance to achieve 30% refusal. Total intake was estimated by marker technique (Cr2O3). Live weight was recorded twice a month. Data were subjected to statistical analysis based on general linear model suited to a completely random design. The results showed that growth rate of Bali heifers grazing native pasture during wet season in Timor was low (35 g/d), and providing high quality forages as supplements at night significantly improved LWG by up 205%. Yet, the magnitude of LWG response varies according to type of forage, with the best result achieved by heifers given Gliricidia sepium leaf. Key Words: Bali Cattle, Grazing, Native Pasture, Wet Season, Supplement

208 - 215

Authors: E.T. Setiatin, D. Sajuthi, B. Purwantara, C. Talib

Pregnancy-associated glycoprotein (PAG) structurally related to aspartic protease, expressed in the outer epithelial cell layer (trophectoderm) of ungulate placenta. Ovine PAG (ovPAG) synthesized by mono- and binucleic trophoblast before complete implantation at Day 14-15. Of this, ovPAG could be used as a marker for early pregnancy. The objective of study was to extract and isolate PAG from placenta of Garut Sheep collected at term and to characterize their molecular weight. The procedures included extraction of protein at neutral pH (cotyledon was thawed, minced, added PBS, blended and centrifuged), acidic (H3PO41M, pH 4,5; centrifuged) and ammonium sulfate (40% and 80% (NH4)SO4, centrifuged) precipitation; gel filtration (Sephadex-G75), anion exchange chromatography (DEAE- cellulose). Cotyledon extract was subjected to Sephadex-G75 and DEAE cellulose, and their fractions were measured their absorbances. Absorbances of Sephadex-G75 and DEAE fractions at peak were assayed for protein concentration (Bichinconinic protein assay). Continuously, these fractions were subjected to monogel SDS-PAGE and stained by Commassie Brilliant Blue. It was four different molecular weights isolating from cotyledon of Garut Sheep, namely 68.8, 36.04, 32.39 and 12.18 kDa. However, after chromatography anion exchange (DEAE-cellulose), only three bands exist, those were 71,67; 33,64 and 30,86 kDa. Key words: Garut sheep, Pregnancy-Associated Glycoprotein (PAG), Cotyledone, SDS-PAGE, DEAE-cellulose

222 - 229

Authors: C. Sumantri, D. Herdiana, A. Farajallah, D. Rahmat

Pituitary-Specific Transcription Factor-1 (Pit-1) is a transcription factor with critical role in the transcriptional regulation of multiple genes in the pituitary. The objective of this research was to identify polymorphism of Pituitary-Specific Transcription Factor-1 (Pit-1) gene at Locus (Pit-1-Hinf1) and to investigate any possible associations of Pit-1 genotypes on dam body weight, milk production and milk quality in local sheep at the Jonggol Animal Science Teaching and Research Unit (JASTRU), Fact. Anim. Sci. Bogor. Agric. Univ. A total number of 161 blood samples were collected from 3 local sheep, namely Garut from Wanaraja (55 hd), Garut from Margawati (23 hd) and lactating ewes (83 hd) from JASTRU farm in Bogor. Genomic DNAs were extracted by a standard phenol-chloroform protocol and amplified by a polymerase chain reaction (PCR) techniques, then PCR products were digested with a Hinf1 enzyme restriction. Fragments of Pit-1gene at locus Pit-1-Hinf1 was detected by a silver-staining method. A length of 637 base pairs (bps) of the Pit-1 gene of local sheep was successfully amplified. The Hinf1 restriction enzyme cut the PCR product into three different length of fragments succesively at 345, 137, and 115 bps designated as A allele; whilst B allele had four fragments at 283, 137, 115, and 62 bps respectively. The locus of Pit-1-Hinf1 was polymorphic in local sheep from Jonggol, however it was monomorfic in Garut sheep. The frequencies of A and B alleles were 0,806 and 0,194 respectively. Pit-1 genotypes had no significant effect on dam body weight and milk production. This result is indicating that the use of single locus Pit-1-Hinf1 in Pit-1 gen is less effective to be used as a candidate in selecting dam body weight and milk production in these three local sheep. Key words: Local Sheep, Pit-1 Gene, Polymorphism, Dam, Milk

237 - 243

Authors: Widodo Suwito

Verotoxigenic Escherichia coli (VTEC) strains are responsible for serious human illnesses. These strains are commonly found in milk. The aim of this study was to determine the occurrence of verotoxigenic E. coli in milk. A total of 351 milk samples, were collected from dairy farms in Bogor, Sukabumi and Cianjur. These samples were analyzed for VTEC using biochemical, serological and vero cell cytotoxicity assays. VTEC O157:H7 isolates were found in milk collected from dairy herds in Bogor and Sukabumi at rates 0.47% of 214 samples, 1.10% of 91 samples respectively, and none in Cianjur. Hemolytic E. coli isolates were found in 0.94% of 214 milk samples from Bogor, 2.2% of 91 milk samples from Sukabumi and none from Cianjur. From E. coli isolates, 53 isolates (67.95%) were verotoxigenic, consisted of: two E. coli O157:H7 isolates and 51 non O157:H7 isolates.Therefore this study showed the occurrence of VTEC in milk samples from dairy farms in Bogor, Sukabumi and Cianjur. Key words: Milk, E. coli (VTEC) O157:H7, Verotoxigenic

83 - 89

Authors: Ema Damayanti, Ahmad Sofyan, Hardi Julendra, Tri Untari

The aim of this research was to study the use of earthworm meal (TCT) L. rubellus as anti pullorum agent in poultry feed additive (IP). The antibacterial activity of TCT against Salmonella pullorum was examined using diffusion agar method at each of the following concentrations: 0, 25, 50, 75 and 100% (w/v) in 100 µL DMSO. In vivo test was conducted using 80 broiler chicken and were infected by S. pullorum with treatments of: IP0: IP contained 0% TCT, IP1: IP contained 25% TCT, IP2: IP contained 50% TCT, IP3: IP contained 75% TCT and IP4: IP contained 100% TCT. Each treatment was replicated 4 times with 4 chicks each. Feed additive was periodically fed to broiler during 7 days before and 10 days after infection. Anti-pullorum activities were evaluated using serology test, isolation and biochemical identification of S. pullorum. The results showed that 75% TCT was optimum to inhibit S. pullorum in vitro. The isolation and identification of S. pullorum results showed that 0 out of 8 (0%) broilers treated with IP4 was not infected by S. pullorum whereas 1 out of 2 (50%) broilers treated with IP0 were infected by S. pullorum. The reduction of S. pullorum prevalence as followed by increasing TCT in feed additive. In conclusion, TCT as poultry feed additive could inhibit S. pullorum infection. Key words: Earthworm Meal, Feed Additive, S. Pullorum

97 - 103

Authors: Achmad Gozali Nataamijaya

A study on the performance of Nagrak and Kampung chicken under intensive management system was conducted in Cibadak District of Sukabumi West Java. As many as 200 hens of Nagrak and Kampung, each were placed in individual cages. The birds were given 90 g of diet daily, the diet was the mixture of layer commercial diet and ricebran at equal ratio, drinking water was given ad libitum. Artificial insemination was conducted every three days using semen collected from 20 cockerels of each local bird. Disease control was done by vaccination against Newcastle Disease and Infectious Bursal Disease. Sulfamix and antibiotics were given whenever needed. Eggs were collected twice a day, weighed and stored to be incubated or to be analyzed for their characteristics. Parameters observed were physical appearance, hen-day egg production, egg quality, egg fertility and hatchability, body weight, feed conversion and mortality. Results showed that Nagrak chicken physical appearance was different from that of Kampung chicken, yet their hen-day production were not significantly different (26.93 ± 14.10% vs 27.04 ± 16.20%). The egg characteristics of these birds (Nagrak vs Kampung) were as follows: egg weight (36.29 ± 6.50 g vs 35.55 ± 5.42 g); yolk weight (16.61 ± 1.34 g vs 16.22 ± 2.11 g); albumen weight (17.31 ± 2.64 g vs 16.87 ± 1.35 g); shell weight 37 ± 0.81 g vs 2.46 ± 0.54 g); yolk color (9.62 ± 1.81 g vs 9.67 ± 1.70 g); haugh unit (83.60 ± 5.41 vs 83.45 ± 6.10); shell thickness (24.0 ± 0.83 µm vs 24.4 ± 0.67 µm), however no significant difference was found. Neither the egg fertility, hatchability nor mortality rate of the birds was significantly different. At 12 weeks old the average body weight of male Nagrak chicken (1260.04 ± 57.33 g) was much higher (P<0.01) than that of male Kampung chicken (750.68 ± 60.11 g) while the average body weight of female Nagrak chicken (980.37 ± 48.11 g) was much higher (P<0.01) than that of female Kampung chicken (656.11 ± 58.47 g). The feed conversion ratio of male Nagrak chicken (4.21) was better (P<0.05) than that of male Kampung chicken (5.62), the feed conversion ratio of female Nagrak chicken (5.15) was also better (P<0.05) than that of female Kampung chicken (6.81). No significant difference was found on the feed conversion ratio of Nagrak hens (9.24) and Kampung hens (9.06). Key words: Performance, Nagrak, Kampung, Local Chicken

110 - 117

Authors: Marthen L. Mullik, Dennis P. Poppi, Stuart R. Mclennan

The rate of rumen microbial crude protein (MCP) supply to the intestines is a crucial element in the current rumen models to predict respond of ruminants to a certain diet. Data from tropical pastures are always below predicted results from the existing rumen models. Thus, quantification of the rumen MCP supply from tropical forage will improve predictive rate under tropical feeding conditions. Four Brahman crossbred steers (457 ± 20.1 kg) were used in a metabolism study. Pangola grass (Digitaria erianthe cv. Steudal) was harvested every morning and fed to the animals soon afterward. Parameters measured were EMPS, intake, fractional passage rates, and rumen ammonia concentration. The EMPS was estimated using purine derivative excretion in urine. Mean crude protein and water soluble carbohydrate was 6.3 and 7.4% of dry matter (DM) respectively. Mean DM intake was 1.6% liveweight. Average rumen ammonia concentration was 69 mg/L whilst rumen passage rates were 7.84 and 6.92%/h for fluid and solids respectively. Mean EMPS was only 72 g MCP/kg digestible organic matters. It is concluded that EMPS in steers consuming green pangola grass was at the level below the minimum recommended value for forage diets adopted in the current feeding standards. Key words: Microbial Protein, Efficiency, Tropical Grass, Cattle

131 - 141

Authors: A. Anggraeni, C. Sumantri, A. Farajallah, E. Andreas

Genotipe of BB kappa kasein (ĸ-kasein) gene positively influences high milk protein yield. The aim of this research was to verify the use of the к-casein gene as marker of assisted selecion (MAS) of high protein yield in domestic Holstein-Friesian (HF) cattle. Genotyping blood samples was conducted for lactating cows from BPPT-SP Cikole (82 head) and a number of small holders in KPSBU Lembang (190 head). Genotyping the к-casein gene was also conducted for active and non active AI bulls (HF) from BIB Lembang (25 head) and BBIB Singosari (32 head). Varian genotipes were identified trough DNA migration, whereas frequencies of alleles were calculated by Nei Method (1987). The effects of variant genotypes on protein yield and other milk component yields were studied in lactating cows (56 head in BPPT-SP Cikole and 111 head in KPSBU Lembang) by applying Least Square Means (LSM) method of the General Linear Model (GLM). Genotyping the ĸ-kasein gene resulted in three genotypes, namely AA, AB and BB, with two alleles, namely A and B. It was identified that a very limited number of lactating cows with BB genotype (0-9%). These cows produced higher protein yield around 3.37-3.84% than that of AA cows, while AB cows produced protein yield in between, though these differences were not statistically significant (P>0,05). In contrast, AA cows tended to produced higher milk fat yield than BB cows. Cows BB and AB seemingly produced higher dry matter, while both specific density and pH were not affected by variant genotypes of the к-casein gene. The results showed that BB genotype of the к-casein gene tended to be quitely consistent in controlling high protein yield, so it would be a good oppurtinity to be used as MAS of milk protein yield in HF domestic. Key words: Protein Yield, Dairy Cattle (HF), к-Casein Gene, Genotype BB

150 - 166

Authors: Sri Usmiati, Miskiyah ., Rarah R.A.M.

Inhibition technology of microorganism on meat can be done biologically and chemically. Biologically, inhibition to microbe can be conducted by addition antimicrobe, for example bacteriocin that have character as biopreservative. The aim of this research was to study the microbiological quality of fresh meat with bacteriocin isolated from Lactobacillus sp. SCG 1223 storaged at certain addition and temperature with different storage time. This experiment was done based on Completely of Random Design (CRD) with factorial arragement 3 x 4 for meat kept at room temperature (270C) and 3x3 for meat kept at cold temperature (4ºC) in three replications. The first factor was addition of biopreservative: addition bacteriocin (B), without addition antimicrobia (TB), and addition of nisin (N) on fresh meat. The second factor was different storage time at room temperature (H0, H6, H12 and H18) and low temperature (D0, D14, D28). Other treatments as indicators was contaminated with indicator bacteria (S. thypimurium, E. coli, L. monocytogenes). Variable analyzed were initial TPC (Total Plate Count) and total indicator bacteria S. thypimurium; E. coli; L. monocytogenes at fresh meat, meat quality consist of total indicator of bacteria, pH value of meat; and protein level. Result indicated that bacteriocin from Lactobacillus sp. SCG 1223 isolat could inhibit bacteria growth (S. thypimurium, L. monocytogenes, and E. coli. Bacteriocin produced by Lactobacillus sp. can work at room temperature (270C) and cold temperature (40C). Nisin effectivity almost same to bacteriosin produced from Lactobacillus sp. SCG 1223 isolated from cow fresh milk in inhibiting Gram positive L. monocytogenes. Key Words: Microbiological, Bacteriocin, Lactobacillus sp. Var. SCG 1223

1 - 10

Authors: Syahrir Akil, Wiranda Gentini Piliang, C. Hanny Wijaya, Desianto Budi Utomo, I Komang Gede Wiryawan

The change of life style influences human health and it contributes to many human diseases outbreak, therefore nutrition of antioxidant is required. This study was aimed to get an optimum level of combined organic selenium, inorganic selenium and vitamin E in obtaining the best production and reproduction of quails and the highest antioxidant level in quail eggs. This study was conducted from January to August 2008. Numbers of observed quails were 720 individuals (360 female and 360 male quails). The treatments were applied when the quails were six weeks old. Nine treatment diets were: To (commercial diet), T1 (0.46 ppm inorganic Se + 43.50 ppm vitamin E), T2 (0.46 ppm inorganic Se + 87.00 ppm vitamin E), T3 (0.92 ppm inorganic Se + 43.50 ppm vitamin E), T4 (0.92 ppm inorganic Se +87.00 ppm vitamin E), T5 (0.46 ppm organic Se + 43.50 ppm vitamin E), T6 (0.46 ppm organic Se + 87.00 ppm vitamin E), T7 (0.92 ppm organic Se + 43.50 vitamin E) and T8 (0.92 ppm organic Se 0.92 + 87.00 ppm vitamin E). The design of the experimental applied was a factorial – nested design. Any significant differences among the treatment diets were analysed using Duncan's test. The result of this study indicated that treatment T7 (0.92 ppm organic selenium + 43.50 ppm vitamin E) in general gave the highest content of selenium in meat, in egg albumin, egg yolk, vitamin E in egg yolk, glutathione peroxidase (GSH-Px), activity of antioxidant, hatchability and low mortality. Key Words: Quail, Selenium, Vitamin E, Antioxidant

19 - 24

Authors: Sofjan Iskandar, S.D. Setyaningrum, Y. Amanda, Iman Rahayu H.S

One hundred and eight pullets of 13 weeks old white Tangerang-Wareng, were allocated to numbers of wire cages. Each cage had 4050 cm2 floor space. The treatments were three cage-densities (4, 6 and 8 pullets/cage) with six replications for each treatment for growth observation, while there were other three treatments (observation times), which were 07.00-08.00 (morning), 12.00-13.00 (noon) or 17.00-18.00 (afternoon), that were applied and replicated in three for behavior observation. Commercial ration containing 20.86% crude protein, 3.22 Ca, 0.87% total P and 2982 kkal metabolizable energy (ME)/kg and drinking water were provided ad libitum. Results showed that cage density neither significantly (P<0.05) affected birds’ final bodyweight, bodyweight gain, feed consumption nor feed conversion ratio. Analysis of variance showed that there was no interaction effect of cage densites and observation times on every observed behavior variable, except on standing activity. Cage density did not significantly (P<0,05) affect behavior, except behavior of cleanning, which increased with the increase of space allocation. The presentages of eating and pecking were significantly increase in the morning, while presentage of bird having a rest increased at noon. Drinking behavior was not affected by both cage density and observation time. The most birds showed the highest activities in the morning. In general the most cage density of 8 birds/4050 cm2 of floor space (506 cm2/bird), was more likely comfortable to the birds to live. Key words: Cage Density, Tangerang-Wareng Pullet, Growth, Behavior

36 - 44

Authors: Kiston Simanihuruk

Processing of passion fruit (Passiflora edulis Sims f. edulis Deg) to produce passion fruit juice produce passion fruit hulls that is potential for feedstuff. Twenty male kacang goats (average initial body weight 17±1.24 kg) were used in an experiment to study the effect of utilization of passion fruit hulls as feed component in the complete pellet ration on their growth. The experiment was arranged in completely randomized design consisting of 4 diets and 5 replications. Animal were randomly allocated into 4 diets (0, 15, 30, 45% level of passion fruit hulls). Each diet contained 14% crude protein and 2550 Kcal kg-1 metabolism energy. The ration was offered at 3.8% of body weight based on dry matter. The result of the experiment showed that all variables observed were not affected by level of passion fruit hulls (P>0.05). Average daily gain and feed efficiency tended to decrease with the increase level of passion fruit hulls. The highest dry matter, organic matter and N intake (768,78 g h-1 d-1; 687,37 g h-1 d-1 and 17,22 g h-1 d-1 respectively) were found from R1 treatment (15% level of passion fruit hulls). It was concluded that passion fruit hulls can be used till 45% level in the diet of Kacang goat. Key Words: Passion Fruit, Complete Pellet Ration, Kacang Goat

50 - 57

Authors: A. Wicaksono, R.I. Arifiantini

The quality of chilled semen depends on the composition of diluent. The choice of the buffer, anti-cold shock and nutrition sources can be the first decision in order to choose appropriate diluents. Nowadays a lot of diluent are used for canine semen preservation such as Tris buffer and Citrate buffer. This study was aimed to observe the differences of diluent for preserving Retriever dog spermatozoa. The semen sample collected from four Retriever dogs with three times repetition. The semen was evaluated macro-and microscopically. The semen with >70% sperm motility was divided into four tubes and diluted with diluter 1 (P1), diluter: P2, P3 and P4 (modified P3). The diluted semen was divided into two tubes and each sample was stored at room and 50C temperature. The viability of chilled semen was observed every 3 hours at room temperature and 12 hours at 50C. The result showed that P2 keep the sperm viability better than the other diluents. On 50C at 24 hours storage P2 showed the highest motile and live sperm percentage (46.25 ± 0.22%; 57.11 ± 0.25%). In room temperature at 6 hours P2 showed the highest motile and live sperm percentage (40.94 ± 0.20%; 52.65 ± 0.23%). It is concluded that P2 can keep the sperm viability by 84 hours of 50C and 21 hours at room temperature. Key Words: Diluents, Dog Sperm, Retriever

66 - 74

Authors: Muharam Saepulloh, I.W.T. Wibawan, D. Sajuthi, D. Setiyaningsih

Different subtypes of bovine herpesvirus 1 (BHV-1) have been associated with different clinical conditions of cattle. For that reason subtypes differentiation has become an essential tool for understanding the pathogenesis and epidemiology of BHV infections. In search for a genomic region that would allow a clear distinction between BHV-1.1 and BHV-1.2 of glycoprotein D (gD) genes of 8 Indonesian isolates were amplified and sequenced. The amino acid sequence alignments revealed that the levels of genomic similarity ranging from 98.8 to 100% among BHV-1 Indonesian isolates and its results were also similar between BHV-1 Indonesia isolates and BHV-1.1 reference, and 98.4 to 98.8% between BHV-1 Indonesian isolates and BHV-1.2 reference. The isolates could be clearly separated into BHV-1.1 and BHV-1.2 after phylogenetic analysis. The results showed that the Indonesian isolates were characterized as BHV-1.1 as agent caused respiratory tract infections in cattle or infectious bovine rhinotracheitis (IBR) disease. The results suggest that the phylogenetic analysis performed here can be used as a potential molecular epidemiological tool for herpesviruses. Key Words: BHV-1.1, BHV-1.2, Glycoprotein D, Phylogenetic Analysis, IBR