European Journal of Medicine. Series B

3-15

Authors: Ulugbek G. Gaiibov, Esokhon Dzh. Komilov, Nurali A. Ergashev, Rakhmatilla N. Rakhimov, Nodira G. Abdullazhanova, Muzaffar I. Asrarov, Takhir F. Aripov

Functional parameters of mitochondria, such as cyclosporine sensitive megapore (mPTP), ATP-dependent K + channel (mitoKATP), as well as inhibition of free-radical membrane oxidation under the conditions of normoxia play an important role in the functioning of mitochondria, and the cell as a whole. Disruption of the normal functioning of these parameters occurs in various pathological conditions. Thus, to study the effect of a new polyphenol compound 1,4,6 tri-O-galloyl-2,3-valoneyl-β-D-glucose, as an object of adjusting the functions of mitochondria in pathological conditions, in this paper we investigated the effect of this compound on ATP -dependent K + channel and on cyclosporic sensitive time (mitochondrial permeability transition pore) in mitochondria from rat liver. Also, the study of the antioxidant activity of 1,4,6 tri-O-galloyl-2,3-valoneyl-β-D-glucose showed that this compound has a high antioxidant activity in Fe2 ++ ascorbate-induced mitochondrial swelling.

25-49

Authors: Shunkor S. Khushmatov, Pulat B. Usmanov, Khurshida K. Nadjimova, Ulugbek G. Gayibov, Nodira G. Abdulladjanova

The aim of the present study was to determine mechanism of the inotropic effect of (–)–epigallocatechin 3–O–gallate (EGCG) in the rat myocardium. EGCG was isolated from seeds grapes (Vitis vinifera L.). Isometric tension forces of preparations of isolated papillary muscle were recorded using a force transducer (Type F30/Model D–79232; Hugo Sachs Elektronik, Germany). EGCG (15–100 µmol.L–1) showed dose–dependent a positive inotropic effects in rat papillary muscle contractility. The condition where the maximal effected concentration (150 µmol.L–1) of EGCG, the isometric developed force of papillary muscle preparation was decreased from 67,5±6,2 % in comparison with the control group (p<0,05; n=3–4). In these conditions, the EC50 value (the values of concentration for 50 % of the maximal effect) EGCG was 22,39 µmol.L–1 or pD2 (–logEC50)=4,652. In the experiments lead by means of specifical experimental technique of a mechanical stretching of myocardium showed, that EGCG (150 µmol.L–1) was increased amplitudes II phase (SFR, slow force response) from 33,7±4,9 % in comparison with the control group. Pretreatment with amiloride hydrochlorid (1 mmol.L–1), a nonselective inhibitor of NHE–1, attenuated the positive inotropic effect of EGCG in the isolated rat papillary muscles. In conclusion, the present study demonstrates that, the positive inotropic effect of EGCG (15–150 µmol.L–1) in isometrically contracting papillary muscles seems to be mediated by NHE–1 [Nа+]in NCX1 [Са2+]in pathway. These data may serve as a basis for further detailed pharmacological mechanism of action of this compound.

56-60

Authors: Nana K. Natsvlishvili, Lela Kiparoidze

Results of microbiological investigation of catarrhal and ulcerous gingivitis on the background of treatment with Unimag are presented in the work. Preparation Unimag is a stable suspension of highly-dispersed magnetic particles. Unimag is magnetic-sensitive, X-ray contrast, bactericidal substance; it is characterized by ability to increase functional activity of phagocytes, absorption of various bio-macromolecules, and high penetration ability in tissues. Studies have revealed that treatment with Unimag of the patients with catarrhal and ulcerous gingivitis, rapidly normalizes quantity of microbes in the oral cavity, substituting the gram-negative pathogenic flora for the gram-positive microorganisms in the oral cavity. Unimag increases sensitivity of pathogenic flora towards the anti-bacterial preparations. All the above-mentioned is significant for efficient impact on damaging factors during inflammation.

68-72

Authors: Victoria A. Sergeeva, Julia O. Kharkova, Ekaterina A. Ivanova

American physician Philip Hench, who first used glucocorticosteroids (GCS) to treat patients with rheumatoid arthritis, underwent a revolution in medicine and was awarded the Nobel Prize in 1950 for his work. Since then almost all have become known about the GCS. Doctors working with the GCS often respond about such therapy as walking on the razor's edge. Alas, there are clinical situations when it is impossible to avoid high doses of these drugs, in other cases there are always trade-offs. The appointment of GCS should be preceded by an in-depth analysis of the need and justification of the choice of such therapy, because sometimes the severity of side effects and complications after treatment with GCS exceeds the severity of the initial disease. In this article, we present our own clinical observation of a patient with iatrogenic Itsenko-Cushing syndrome, which resulted from a long illicit therapy with prednisolone.