European Journal of Medicine. Series B

62-70

Authors: Orifjon A. Aripov, Liliya R. Sha-Akhmedova, Zakhro A. Usmanova

There were studied immunological parameters in patients with chronic viral hepatitis B (CVHB) and it was evaluated the effectiveness of flavonoids and phospholipid preparation. There was a decrease in CD3+, CD4+, CD8+ - of lymphocytes, CD4/CD8, CD16+- of cells. The content of CD95 + and TNF-α was significantly increased at all degrees of activity of the cytological process. It was established an increase of the concentration of all types of immunoglobulins (IgA, IgG, IgM) in children. It was established tendency of normalization of the studied parameters in children taking flavonoids, but the level of TNF-α remains high for all therapeutic approaches.

77-99

Authors: Oleg V. Gradov

A simplified theory of the optical signal registration using lab-on-a-chip with CMOS/CCD detectors is described. The difference between the contact active and passive chips with optically different possible registration schemes is outlined. For passive chips a possibility of their introduction into a sandwich-like structure with photoelectronic multipliers is also considered (a design of a so-called lab-on-a-PEM similar to the earlier proposed lab-on-a-tube technique where it is possible to integrate the glass surface of the vacuum tube with a microfluidic chip. It is also indicated the possibility of a multiparametric contrastimetry on a chip with a system converting arbitrary physical signals to an optical one. This technique allows to measure contrast, gradient and colocalization of the primary variables (converted signal) distribution in 2D mode with spatial resolution. Either a photoinjection or a photoemission method of estimation of the parameters related to the primary analytical signal for PEM-combined labs-on-a-chip is also proposed. For the simplest “boundary calibration” of the optical CMOS-/CCD-based chip it is suggested to use either white or black balance/level, which after recalculation within a single COBAC system can be applied for estimation of the primary non-optical signal parameters together with contrastimetry. Also an “energy calibration” method is described combining calibration with the photocell and a thermoelement (the parameter measured in this case is specific thermodynamic irradiance). A simple simulation of the light transmission through a biological sample located on a chip to the chip surface was performed using Geant4 software developed by CERN (Scientific Linux OS). The following topology of the on-chip experiment is proposed: a sandwich-type chip system including a light source (LED), emitting photons from the point («World initStep»), a biological cell with cytoplasm («World») and an osmotic vacuole («Tank»), which is a part of the optical path from the light source to the detector of CCD or CMOS chip, which is considered as a target («OutOfWorld») and serves as a substrate for the biological sample. The possibility of the polarization parameter registration at the given experimental scheme and the path geometry is also mentioned. Due to the possibility and the initial application of the Monte-Carlo methods in Geant4 for the nuclear physics, the above model can be extrapolated towards the lab-on-a-chip with either a scintillation primary converter or without it (the operation principle of such a chip is based on the SEU – Single Event Upsets upon the single particle interaction with the pixels). It is also shown that Haar transforms and Fourier registrograms of the line scans from the CCD/CMOS can be considered as one of the sources of visualizable descriptors (both of the optical and primary non-optical signals).

4-9

Authors: Maradi A. Burduli

The paper presents the research results achieved by creating a model of experimental pulpitis model and the influence of drugs. For the exposure there is used the propolis and retabolil. Based on data from their experimental research, it can be concluded that in the pulp chamber, the creation of the experimental pulpit model, cells of the connective tissue from the stable state were removed, and by the action of biologically active substances – propolis and retabolil – there were created conditions like gonads. As a result, division and differentiation of the cells were obtained by the figures of meiosis. Therefore, when two haploid single-cell cells of one organism merge, a zidopod-like cell will be obtained, which will be capable of (can be able to) develop and give rise to cellular populations.

16-22

Authors: Sergey A. Sherstyuk, Svitlana A. Nakonechna, Maryna O. Ivanenko

There was studied the influence of neonols AF 9-12 and AFS 9-6 KM on parameters receptor connection of adrenal, serotoninal, dofaminal and glucocorticoid receptors of celebral cortex in subacute toxicological experiment on white rats Vistar population, that were exposed to 45 days feeding in dozes 1/100 and 1/1000 DL50. The revealed alterations on the part of 1-adrenoreceptors were characterized by inerese of affinity to lygand and decrease of number of this type of receptors. There was observed the increase of number of highly afinal and decrease of low afinal pool -adrenoreceptors in experimental groups of animals. The parameters of connection of serotoninal receptors of second type were chanding in affinity of connection with lygand. In animals of experimental groups were changed affinity D2-receptors to the marked lygand in cortex in comparison with control. Xenobiotics inereased the presence of glucocorticoid receptors in celebral cortex.

30-58

Authors: Evgenii D. Adamovich, Pavel L. Alexandrov, Oleg V. Gradov

This paper provides a new approach for ion channels activity registration in noisy conditions with stochastic incoherent open-close transitions on membranes. The required result is obtained using phase-selective nanovoltmetric techniques with frequency discrimination. This technical principle is the foundation for different phase-selective (φ-path-clamp) methods, based on vector voltmeters or lock-in nanovoltmeters for indication of the real and imaginary components of the complex electrophysiological signals in the ion channel activity.