European Journal of Medicine. Series B

80-85

Authors: Muhammad Sohail Anwer, Sumbul Shamim, Salman Ahmed, Mohammad Mohtasheemul Hasan, Iqbal Azhar

Melilotus officinalis (L.) Pallas., is used traditionally to treat hypertension and chronic venous insufficiency. The high prevalence of hypertension and the side effects of antihypertensive drugs potentiate us to evaluate Melilotus officinalis. The hypotensive effect of Melilotus officinalis butanolic fraction (MOBF) was investigated in anaesthetized normotensive rats. The doses of 60, 80 and 100 mg/kg, i.v., produced a significant (P<0.05) decrease in blood pressure. The statistically non significant reduction in blood pressure was showed by 20 and 40 mg/kg, i.v. These results suggest that Melilotus officinalis exhibited hypotensive effect in normotensive rats. However further evaluation of compound related activity is required.

92-103

Authors: Afrooz Habibi, GholamReza Ghazipour, Davoud Dehghan Tarazjani

Background The survival rate of a cardiac arrest victim increases with basic life support (BLS) as the first level of medical care. The purpose of this study was to compare BLS knowledge and skills of medical university staff before and after training course. Methods Thirty eight medical university staff participated in a BSL training course. The course included the assessment of participant’s knowledge by a pretest questionnaire, a lecture, hands-on training in BLS skills, a post-test questionnaire and an interview to evaluate their attitudes. Data were analyzed by the SPSS software version 19, using t-test, one-way ANOVA and chi square tests. P value less than 0.05 was considered statistically significant. Results Our results demonstrated that the mean knowledge score was 6.00 ± 2.09 vs. 8.26 ± 2.10 in pre- and post- education, respectively. A significant mean gain score (2.26) from the pre-test scores to post-test scores was statistically observed (P<0.05). According to the results, 23.68% of trainees had an ineffective level and 76.32% of them had an effective level. No differences were statistically found between trainees’ gain scores and effectiveness level in terms of gender, age, work experiences and the prior first aid training experience (p>0.05) whereas, there were the significant differences between trainees’ gain scores and, education and career fields(p< 0.05). Our result also presents that staff attitudes toward the training were positive. Conclusion The present research shows the BLS training had a positive impact on the knowledge and attitudes among university staff. BLS training is extremely useful for medical university staff.

111-117

Authors: Iryna O. Moiseyenko, Lyudmyla N. Prystupa

The C825T polymorphism of β3-subunit G-protein gene (GNB3) is associated with activation and cell growth that lead to left ventricular hypertrophy (LVH). The aim this research was to analyze the association between the C825T polymorphism GNB3 gene and some central hemodynamic parameters, left ventricular mass (LVM) and heart geometric in patients with essential hypertension (EH) and LVH. We used two-dimensional-controlled M-mode echocardiograms in 155 patients whith EH for evaluation of LVH, the C825T polymorphism GNB3 was determinated by polymerase chain reaction and restriction digestion. 49 (0.32) were homozygous for the C allele (CC), 86 (0.55) were heterozygous (CT), and 20 (0.13) were homozygous for the T allele (TT). C- and T alleles carries were 32% and 68% respestively, T allele frequency was 0.4. The differences between left ventricular internal end-diastolic diameter (LVEDD), intraventricular septum thickness (IST), posterior wall thickness (PWT), left ventricular mass (LVM) and left ventricular mass index (LVMI) in the patients with EH according to genotypes and alleles of C825T polymorphism GNB3 gene were statistical significance (p=0.001, p<0.001, p=0.025, p<0.001 and p=0.001 respectively). Normal geometry and LV remodeling (0.48 and 0.44 respectively) prevails among patients with EH versus eccentric and concentric LVH (0.03 and 0.05 respectively). The frequency of the T allele was significantly higher in patients with LV remodeling compare the patients with normal geometry (0.43 versus 0.365 respectively) and didn’t find significant difference of frequency genotypes and alleles for eccentric and concentric types of LVH. Thus, the T allele of C825T polymorphism of β3-subunit G-protein gene is associated with left ventricular hypertrophy, higher some echocardiographic parameters left ventricular, left ventricular mass, left ventricular mass index but not associated with types of left ventricular geometry in patients with essential hypertension in Ukrainian population.