17-23
Histogenesis of Male Gonads in Developing Human Foetuses
Authors: DR. PHAD VAIBHAV V., DR. Mrs. JOSHI RAJNI A., DR. JADHAV SHAILENDRA S., DR. HEREKAR NARSINH G.
Number of views: 431
The male Gonads or Testes are embryologically derived from
“Mesoderm” except for Primordial germ cells giving rise to
spermatogonia which are derived from epiblast. The true sources are the
mesothelium lining the posterior abdominal wall, underlying
mesenchyme & Primordial germ cells. The gross changes occurring
during development of male gonads are studied in much more detail as
compared to histological changes therefore very few studies available on
the same.
40 aborted male human fetuses between 12- 40 weeks of gestational age
with no obvious congenital anomalies were obtained for study. Testes
were removed and histological slides of 5 to 7 microns sections were
prepared and stained with Haematoxylin and Eosin method. Also some
slides were stained with Masson’s trichrome stain & studied under light
microscope. The tunica albuginea & epiorchium were already
differentiated at 12th week of gestational age. Tunica vasculosa appeared
at 14th weeks of gestational age. At 16th week some rete tubules were seen
in mediastinum. At 24th weeks of gestational age complete septae were
seen, and the lobules were completely formed. At 28th week both layers of
tunica vaginalis were seen. Initially dark nucleated spermatogonia were
more numerous than pale nucleated spermatogonia and Sertoli cells.
After 30th week pale nucleated spermatogonia were more numerous
along with Sertoli cells. The findings also showed that testis at term were
not assuming adult architecture. The seminiferous tubules were showing
absence of cells of spermatogenic series. They seem to appear at puberty,
and lumen in these tubules does not appear till term.
Keywords: Mesoderm, Primordial Germ Cells, Tunica Vasculosa, Rete
Tubules, Spermatogonia