Iraqi Journal of Science

9-15

Authors: Alaa Salim Hamzah

This study was aimed to analysis phylogenetic tree of the gene cpn60 in Acinetobacter baumannii that was identified in Baghdad. Study included collection two hundred specimens (fifty from UTI, fifty from wound infection , fifty from respiratory tract infection and fifty from otitis infections) . In primary laboratory diagnosis and confirmed by using VITEK- 2 Compact system, twenty isolates of this bacterium were indentified (10%) from total specimens. Extraction of geneteic material to detect target gene by amplification this target gene. DNA sequencing of all isolates was done. Then alignment of sequencing in NCBI and draw phylogenetic tree by use Geneious 9 software among sequence of locally isolates . The results in phylogenetic tree of cpn60 gene in locally isolates showed 4 groups of isolates different with difference source of isolation. Then phylogenetic tree for locally isolates and high identity global isolates in gene bank was drew and its results showed 12 locally isolates not identity with standard isolates. So, chosen isolate (AE_29) isolate from these 12 isolates and documented in national GenBank as anew isolate under accession number (LOCUS KY818056 ) of nucleotides sequence and protein ID "ARV90994.1"

25-29

Authors: Jenan Atiyah Ghafil

Biofilm formation represents one of the biggest problems facing scientists because of this phenomenon linkage with virulence of bacteria and other clinical environmental problems. In the present study, two clinical isolates, Escherichia coli, and Staphylococcus aureus were exposed to the non thermal plasma for different intervals of time (1, 2, 4, 8, and 16 min). The biofilm was measured post exposing. It was found that 2 min. exposing to non-thermal plasma reduced the biofilm formation by both clinical isolates significantly. It can be concluded that the ability of S. aureus to form biofilm higher than E. coli and exposing for 2 min to non-thermal plasma sufficient to reduce the biofilm formation by both isolates significantly

38-43

Authors: Zaid Akram Thabit

This work was evaluated the antioxidant, antibacterial and cytotoxic activity of Costus speciosus rhizomes methanol extract. The FLC analysis showed the presence of five compounds in the methanol extract of C. speciosus rhizomes. These compounds were Quercetin (5.2mg/ml), Rutin (6.02mg/ml), Luteolin, (18.3mg/ml), Kaempherol, (11.34mg/ml) and Coumarin (1.41mg/ml). The maximum antioxidant activity of the extract was at concentration 1000 μg/ml with free radical scavenging activity approximately 67.5%. It was less than standard ascorbic acid 85.5% and Gallic acid 90% with significant difference (p≤0.05), with no significant difference in comparison with standard TBA 68.5%. The IC50 of extract was 3093μg/ml, while the IC50 of ascorbic acid, Gallic acid and TBA were (277.2, 364.5 and 601.3 μg/ml) respectively. The extract revealed influential growth inhibition for all bacteria used in this experiment. The extract was moderately effective at concentration 400 μg/ml of extract with inhibitory activity 50.7 % on MCF-7 cell line and IC50 139.1 μg/ml.

57-66

Authors: Alwan M. Alwan, Rasha B. Rashid, Amer B. Dheyab

In this work, the fine structure macro-porous silicon (macroPS) substrate was prepared by photo-electro-chemical etching of n-type silicon wafer. Ultraviolet illumination condition of wavelength 360nm wavelength and intensity of about 100mW/cm2 with etching current density of about 50 mA/cm2 and etching time 5 min was employed. The Hybrid device gold nanoparticles /macroPorous Silicon (AuNPs/macroPS) was fabricated by deposition AuNPs into mPS substrate Via immersion plating process of macroPS in the solution of HAuCl4 with the (10-3M) concentration and 2min immersion time. The characteristics of PS before and after immersion process were investigated by scanning electron microscopy (SEM), EDS, X-Ray diffraction (XRD), photoluminescence (PL) and Infrared spectroscopy (FTIR). The J-V characteristics of sandwich structure showed that the maximum sensitivity of the AuNPs/macro PS was about (90.5%)for compared with macro-PS substrate. The current-voltage characteristics were performed in primary vacuum with a base pressure of about 0.2mbar and CO2 with 1mbare concentrations. Significant enhancement was observed in sensitivity of the AuNPs/macroPS hybrid device and temporal response after deposition the AuNPs.

75-85

Authors: Ali A-K. Hussain*, Fatimah Jumaah Moaen

In this work, the Al-Mg alloys plasma plume that produced under vacuum (10-1 torr) by Nd:Yag Laser was studied. the plasma spectra were analyzed by comparing the atomic lines of Al and Mg lines with standard lines. The effect of laser energies on spectral lines produced by laser ablation were investigated using optical spectroscopy .several plasma characteristics like electron temperature, electron density, Debye’s length, plasma frequency and number of particles in the ‘‘Debye’s Sphere’’ were calculated in vacuum .The results show the electron temperature and electron density increase in magnesium, aluminum and magnesium :aluminum alloy targets . It was found that the lines intensities at different laser peak powers increase when the laser peak power increases then decreases when the power continues to increase

96-104

Authors: Muthafar F. Al-Hilli

Lead-free ferroelectric nano ceramics of BaZrxTi1-xO3 (x=0.1, 0.2 and 0.3) were prepared by means of microwave assisted chemical route. The structural, dielectric and electrical properties were examined. The crystalline structure of the specimens was studied by X-ray diffraction patterns. All the samples showed pure single phase of perovskite structure with space group of I4/mcm. X-ray diffraction data illustrated that there is no secondary phases exist. Structural and electrical properties of barium titanate ceramics are influenced significantly by small additions of Zr. The electrical conductivity showed higher values at x=0.2 and decreased at higher Zr content. The Hall charge mobility is found to decrease with Zr addition. The negative sign of the Hall coefficient RH confirms the dominant n-type conduction in all samples. The charge carrier concentration decreased with Zr content increase. Suggested potential application for this ceramics compound is in high storage multilayer capacitors and ferroelectric applications.

119-134

Authors: Qusai Y. Al-Kubaisi, Sabah Ahmed Khorsheed

Groundwater suitability for consumption in the Yaychi area is determined by analyzing groundwater samples of 21 wells distributed in the area, for dry period which begin from May to October 2016 and wet period begin from October 2016 to May 2017. Results indicated that the temperature (T °C) have normal values and the water of low alkaline and excessively mineralized, while most of these samples, for both periods, are slightly – brackish water. The mean ion concentrations for two periods indicates that all major and secondary ions are higher than water quality standards for drinking purposes according to World Health Organization (WQI) and Iraqi Standard (IQS) except for K and Cl ions. The water in the study area is classified as very hard. Kurolov formula shows the presence of three predominant salts MgSO4, Na2SO4 and CaSO4, and the water origin in the area is meteoric water. The predominated of water types are " earth alkaline water with increased portions of alkalis with prevailing sulfate and chloride" and "Normal earth alkaline water with prevailing sulphate or chloride". After comparing the ionic concentrations with the water quality standards for different purposes, it was found that groundwater in the area is unsuitable for human drinking, but it's suitable for building and livestock and for growing most types of crops, it is also suitable for irrigation purposes depending on the sodium adsorption ratio (SAR), Soluble Sodium Percentage(Na%) and the Residual Sodium Carbonate (RSC), and unfit for irrigation according to Magnesium Adsorption Ratio(MAR) index. The water in the study area is devoid of heavy metal pollution except (Fe), (Pb) which are shown in water of some wells.

144-155

Authors: Ali M. Hasan Altameemi*, Aiad Alzaidy

Mishrif Formation regards one of the most important reservoirs in Iraq.Well logging represents one of the most important tool in the formation evaluation. According to the Petrophysical properties that have been gotten from well logging, Mishrif Formation in terms of reservoirs units, consist of several reservoirs units..Major reservoirs units divided into three reservoir units,MA,MB&MC. Each of these major units divided into minor reservoirs units (MB11,MB12,MC2&MC3).MB major reservoir units represent the best reservoir unit.These reservoirs units separated by cap rocks(mainly tight limestone)(CR1,CR2,CR3,CR4,CR5,CR6,and CR7).CPI were demonstrated for all wells.Hydrocarbon saturation vs. water saturation have been determined for each units.In addition, the types porosity and moveable vs.residual oil were calculated.

173-178

Authors: Yossra Hussein, Safa Abdel Jalil

Science, technology and many other fields are use clustering algorithm widely for many applications, this paper presents a new hybrid algorithm called KDBSCAN that work on improving k-mean algorithm and solve two of its problems, the first problem is number of cluster, when it`s must be entered by user, this problem solved by using DBSCAN algorithm for estimating number of cluster, and the second problem is randomly initial centroid problem that has been dealt with by choosing the centroid in steady method and removing randomly choosing for a better results, this work used DUC 2002 dataset to obtain the results of KDBSCAN algorithm, it`s work in many application fields such as electronics libraries, biology and marketing, the KDBSCAN algorithm that described in this paper has better results than traditional K-mean and DBSCAN algorithms in many aspects, its preform stable result with lower entropy.