Biotechnologia Acta

29-41

Authors: O. Tigunova, N. Beyko, G. Andriiash, S. Shulga

Literary data on the organization of the genome, the connection of genes with the production and regulation of solvents, and the metabolic engineering of solventogenic clostridia are presented. The transition from the phase of the formation of acids to the phase of the formation of alcohols and the relationship of the latter with sporogenesis are analyzed. The main key genes (ak, pta, buk, ptb, thl, crt, bcd, BdhAB, ctfAB, adc, rub, Spo0A, adhE, hdb) that affect their course have been identified. The possibility of improving strains by genetic manipulations (inactivation of genes, entering of genes of other microorganisms, etc.) is shown. The effect of gene inactivation on solvent production is shown. An analysis of methods for increasing the accumulation of butanol showed the need to create effective recombinant producer strains for butanol supra synthesis using renewable raw materials.

53-71

Authors: S. V. Gorobets , L. A. Yevzhyk, I. A. Kovalchuk, O. V. Kovalev

The aim of the study was to produce magnetically controlled biosorbent based on fungi of champignon and shiitake, to determine the proportion of the magnetically controlled phase of the biomass of fungi when the magnetic fluid (MF) was added to the substrate and to explore the efficiency of extraction Fe3+ ions by shredded biomass of the fungus. The object of the study was mushrooms champignon Agaricus bisporus and shiitake Lentinula edodes grown in the laboratory. An effective and cheap way to remove waste biosorbent from the working environment is a high-gradient magnetic separation (HGMS), which takes place in high-speed mode. The separation of the magnetically controlled phase of fungi biomass A. bisporus and L. edodes was carried out by HGMS methods. It was investigated that when using the biomass of champignon grown on MF, the properties of the sorbent were significantly improved, the full saturation was 6 times faster in comparison with the biosorbent based on the biomass of the fungus grown without MF.

82-88

Authors: L. Sablii, M. Korenchuk, M. Kozar

The aim of this work was to establish the possibility of Lemna minor usage to improve the efficiency of wastewater treatment from nitrogen and phosphorus compounds. Due to their pollution resistanse, Lemna minor is often used for wastewater remediation. It’s capability of absorption and transformation of various compounds, promotes the deposition of suspended matter, saturates water with oxygen, intensifies the purification processes. Due to the rapid growth rate, duckweed absorbs a large amount of pollutants, thereby purifying water from them. However, there is insufficient data and information on the efficiency of nitrogen and phosphorous compounds extraction by Lemna minor wastewater. That is why the research direction was to determine the efficiency of wastewater treatment from biogenic nitrogen and phosphorus compounds by higher aquatic plants of Lemna minor. The necessary conditions for cultivation of higher aquatic plants of Lemna minor in autumn and winter were established. The efficiency of using duckweed of different mass for purification of waste water from biogenic compounds of nitrogen and phosphorus was investigated. The usage of duckweed together with other higher aquatic plants to investigate wastewater from biogenic nitrogen and phosphorus compounds was investigated. It was found that the efficiency of sewage treatment in the autumn-winter period should adjust the mode of illumination and temperature regime.