Anatolian Clinic Journal of Medical Science / Anadolu Kliniği Tıp Bilimleri Dergisi

1-7

Authors: İsmail DAVARCI , Seniha ŞENBAYRAK , Sebahat AKSARAY , Mücahide Esra KOÇOĞLU, Mert Ahmet KUŞKUCU , Mustafa SAMASTI

Aim Carbapenem-resistant Klebsiella pneumoniae infection has become an important clinical problem with reduced therapeutic options. This study aimed to investigate the carbapenem resistance rates and responsible resistance genes in K. pneumoniae isolates derived from clinical samples collected in Istanbul. Materials and Methods This prospective study included a total of 1452 K. pneumoniae isolates from patients admitted to our hospital between July 2013 and July 2014. VITEK-2 (bioMérieux, MarcyI’Ѐtoile, France) was used for microbial identification and antimicrobial susceptibility testing. The carbapenem-resistant isolates identified by VITEK-2 were also found to be resistant to ertapenem by the ertapenem gradient test. Resistance mechanisms of the carbapenem-resistant isolates were investigated using real time-polymerase chain reaction. Results Of the 1452 K. pneumoniae isolates, 45 (3.1%) were carbapenem-resistant. Of these, 32 (71.1%) were blaOXA-48-positive, 9 (20%) blaNDM-positive, and 1 (2.2%) blaVIM-1-positive. None had the genes blaKPC and blaIMP-1. The greatest susceptibility by the isolated carbapenemase-producing K.pneu moniae was shown to the antimicrobials amikacin and gentamicin. Discussion and Conclusion In our hospital, there are several mechanisms causing carbapenem resistance, and the blaOXA-48 positivity rate of 71.1% is significant. This resistance may spread rapidly and, through enzymatic resistance gene transfer, lead to hospital epidemics difficult to manage. For this reason, accurate and rapid laboratory diagnosis is important in infection control. For faster results, molecular methods, as well as phenotypic methods, must be included in the hospital infrastructure

15-21

Authors: Nihal BÜYÜKUSLU , Zeynep Zehra BİLGİ , Havvanur YOLDAŞ İLKTAÇ , Muazzez GARİPAĞAOĞLU

Aim: This study was planned and conducted to evaluate the effect of nutrition in pregnant women on cord blood fatty acid levels. Materials and Methods: Demographic, anthropometric and nutritional data of 33 pregnant women were taken at the beginning of last trimester and 24-hour food consumption was recorded. Fatty acid content of cord blood was determined by gas chromatography. Nutritional fatty acids were analyzed by BEBIS program and compared with percentage of fatty acids in cord blood. Results: Mean daily energy consumption of pregnant women was 1894,37±590,46 kcal. The daily intakes were 40,7% carbohydrate, 15,5% protein and 43,8% fat. Ratios of saturated ,monounsaturated and polyunsaturated fatty acids in daily nutrition and cord blood were %39,7-%47,5, %38,6-%20,8, %21,7-%32 respectively. Discussion and Conclusion: It was seen that amount of protein taken with nutrition was related with cord blood fatty acids. In this study it was seen that nutrition habbits of increased saturated fatty acids and protein were increasing the cord blood fatty acids levels. Also, it was concluded that pregnant women should have an adequate and balanced nutrition to keep their health and maintain normal fetal growth and more studies are needed in this subject.