International Journal of Medical Laboratory

5

Authors: Alireza Babaei Mazreno *, Gholamreza Sharifi, Mohammad Tollabi

Introduction: The aim of this research was to compare active and passive recovery after a session of intense exhaustive training on the level of serotonin in the serum of the runners. Materials and Methods: In this semi-experimental study, 26 male elite runners were randomly assigned to two groups of active (n=12) and passive (n=14) recovery. Half an hour before the start of the training, 5 ml blood was drawn from the sample and then each subject was tested starting training on treadmill for Bruce test until reaching exhaustion. Immediately and then 10 minutes after the Bruce test, 5 ml of blood was drawn again for measuring serotonin. Results and Conclusions:The mean of serotonin in three steps of before, immediately after and 10 minutes after recovery were respectively 360.83 ± 250.05, 459.67±302.13, 514.5±440.48 ng/ml in the active recovery group and 141.26 ± 92.41, 241.04 ± 104.78, 214.34 ± 120.03 ng/ml in the passive recovery group. There was a significant difference between the amount of serotonin after recovery compared to that of before. However, the type of recovery program after that had no effect on the serum serotonin of the blood of the runners

7

Authors: Seyyede Fatemeh Shams *, Zahra Arian pour, Farzad Molahosseini Foomani, Hossein Gholi Noori

Background and Aims: Human Immunodeficiency virus (HIV) is spreading rapidly among the people worldwide. Infection with this virus leads to immune suppression and finally acquired immune deficiency syndrome. Early HIV detection is dependent on antibody screening against virus by Enzyme Linked Immunosorbent Assay (ELISA). Some confirmatory tests such as Western Blot and Recombinant Immunobloting Assay (RIBA) are used to verify viral infection. Many of the confirmatory test results are indeterminate. The aim of this study was to compare the frequency and patterns of indeterminate results of confirmatory tests in two groups blood donors and patients with high risk behaviors in the northeast of Iran. Materials and methods: It is a cross-sectional study from October 2009 to March 2014, a total number of 1055 serum samples with previous positive HIV ElISA test history were tested in our laboratory, Some by RIBA and some by western blot method. Results: Most of the indeterminate results belonged to blood donors and Western Blot analysis. The most reacting band was P24 in both methods and groups. Conclusions: RIBA assay is more sensitive and reliable than western Blot but it’s necessary to use other supplementary tests with less indistinctive results. It’s necessary to pay attention to HIV glycoprotein reactivity in some methods too.

8

Authors: Mohammad Hosseini-Sharifabad, Fatheme Hajimaghsoodi, Ali Karimzade, Seyedhossein Hekmatimoghaddam *, Mansour Esmailidehaj, Fariba Binesh

Background and Aims: Ecstasy or 3, 4-methylenedioxymethamphetamine (MDMA) is a brain stimulant and a hallucinogenic material prepared by chemical changes in amphetamine. The aim of this study was to evaluate the changes induced by this drug in mouse cardiac histopathology, electrocardiogram (ECG) and blood cell counts. Materials and Methods: In this experiment, 3 groups (n=10) of mice were enrolled. Group 1, as control, received placebo. Group 2 mice were given single daily low dose (20 mg/kg/d for 28 days) of intraperitoneal MDMA, and group 3 were given single daily high dose (40 mg/kg/d for 28 days) of intraperitoneal MDMA. An ECG, aVF lead record was obtained, and then a blood sample was taken for complete blood counts and the heart was removed for microscopic study of tissue sections with routine staining. Results: The group 3 showed significant decrease in erythrocyte indices, myocarditis in 7 cases and monocyte infiltration around cardiac myocytes in 6 cases. In group 2, lower degree of myocardial injury was observed, displaying significant increase in QT and QTc durations in ECG. In high dose group, red blood count, hematocrit, mean cell volume and mean corpuscular hemoglobin concentration showed significant changes with comparison control group. Conclusions: Ecstasy can effect on red blood cell index and can lead to anemia. Many monocytes see around of cardiac cell with increase of ventricular depolarization and repolarization can lead to increase of QRS-QT interval. Combination myocarditis with arythmia and increase of sinus tachycardia show change in cardiac function and myocardial structure, cardiac injury due to hypoxia and ischemic can cause of myocardial infarction.

9

Authors: Gholamabbas Mohammadi, Hasan Azizi *

Background and Aims: Type and saturation of fatty acids can have an important impact on the level of triglyceride, cholesterol, very low and low-density lipoproteins in the blood and thus affect the development of atherosclerosis. Saturated fatty acids have an additive effect on blood cholesterol while for unsaturated fatty acids, a lowering effect has been reported. Fatty acids can have different effects on lipoproteins metabolism and apolipoproteins expression because oils used by human have different compositions. One of the important apolipoprotein is apolipoproteinB ( apoB) .This study was conducted to compare the effect of different nutritious fats on expression of apoB protein. Materials and Methods: For this purpose, 48 Syrian male mice were selected and randomly divided into six groups of eight: chow, diet with10%corn oil, diet with10% olive oil, diet with10% cow ghee), diet with10% sheep ghee, and diet with 2% cholesterol. After two months, liver and intestine were removed and transferred into liquid nitrogen and were frozen at -70ºC. Protein was extracted and the expression of apoB was studied by western blotting. Results: An increase in the expression of intestinal apoB48 was identified in olive oil and cow ghee groups. Hepatic apoB100 expression increased in the cholesterol group compared with the corn oil group. Conclusions: This study indicates that olive oil and cow ghee consumption increase intestinal apoB48 expression.