World Journal of Experimental Biosciences

5-8

Authors: Farah M.Al-Quraishi, Archana Saini

The biofilm formation of bacteria in medical devices especially indwelling urinary catheters considers one of the hospital complications of infections. Here, the anti-biofilm effect of hydrogen peroxide (H2O2) against biofilm produced by Klebsiella pneumoniae on urinary catheters was evaluated. In the present study, 2the five isolates of K. pneumoniae e e were isolated from urine samples of patients suffering from urinary tract infections (UTIs). The biochemical and VITIK technology was used to identify the bacterial isolates. The ability of K. pneumoniae to adhere and form biofilm onto the indwelling urinary catheter was measured. Moreover, the negative effect of different concentrations of H2O2 (0.01%, 0.02%, 0.04%, 0.08%, and 0.16%) on the biofilm formation of K. pneumoniae on the pieces of the indwelling urinary catheter was evaluated in test tubes (in vitro). The result showed that all isolates produced a high level of biofilm. Three isolates were considered moderated biofilm producers and 2 were considered strong biofilm producers. All the used concentrations of H2O2 were effect-negative (P<0.05) on K. pneumoniae (K.p.3). The present study confirmed the anti-biofilm effect of H2O2 on the biofilm formation by K. pneumoniae on the indwelling urinary catheter in vitro.

1-4

Authors: Ghada Mohammed Saleh

Biofilm formation considers a serious problem that faces the physician in treating infectious diseases associated with medical devices. The present study aims to highlight the effect of hydrogen peroxide (H2O2) on biofilm formation by bacteria and yeasts. The current study confirms the anti-biofilm effect of H2O2 on the biofilm formed by bacteria and yeast. The effects of H2O2 on biofilm formation were against the ability of a microorganism to attach to the surface by the effect on either the appendages or the mechanisms of attachment, also it was found that the effect on biofilm can be explained by the effect on the microorganism growth. The current study highlighted the future prospect of using H2O2 by increasing the effectiveness of common antibiotics against high-resistance bacteria by increasing the ways of entering antibiotics by forming pores in the biofilm mass and the microorganism cells.