European Journal of Molecular Biotechnology

4-10

Authors: Pavel A. Krylov

To give the empirical space for development of molecular biotechnologies in regenerative medicine the article presents a systematic review of new data for the last 5 years, dedicated to chondrocyte receptor apparatus, connected to physiological and reparative regeneration in articular cartilage. The main attention is given to the receptors of chondrocytes involved in synthesis and degradation extracellular matrix, differentiation and apoptosis of chondrocytes.

25-40

Authors: Oleg Mosin, Ignat Ignatov, Dmitry Skladnev, Vitaly Shvets

With using of a strain of of L-phenylalanine secreted Gram-positive aerobic facultative methylotrophic bacteria Brevibacterium methylicum В-5652, assimilating methanol via ribulose-5-monophosphate (RMP) cycle of carbon assimilation it was carried out the preparative microbiological synthesis of phenylalanine and metabolically related amino acids (alanine, valine, leucine/isoleucine in the amount of 5–6 mmol/l), labelled with deuterium (2H). The data on adaptation of L-phenylalanine secreted methylotrophic bacterium В. methylicum to the maximal concentration of deuterium in minimal growth medium M9 with 98 % 2Н2O and 2 % [2Н]methanol, and data on biosynthesis of deuterium labelled L-phenylalanine with different levels of enrichment are submitted. The developed method for biosynthesis allows to obtain [2Н]phenylalanine with different levels of isotopic enrichment, depending on 2Н2O concentration in growth media M9, from 17 % (2 deuterium atoms) (on growth medium with 24,5 % 2Н2О) right up to 75 % (6 deuterium atoms) (on growth medium with 98 % 2Н2О) with introduction of deuterium to benzyl С6Н5СН2-fragment of molecule that is confirmed with the data of the electron impact (EI) mass-spectrometry analysis of methyl ethers of N-dimethylamino(naphthalene)-5-sulfochloride (dansyl) derivatives of [2Н]phenylalanine, isolated from the liquid culture after its separation by RP HPLC.