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Features of Glucose Utilization and Oxygen Consumption by Mcf-7 Breast Cancer Cells Depending On in Vitro Culture Conditions
Authors: Ahmed H. Al-Humairi, Oleg V. Ostrovskiy, Ekaterina V. Zykova, Dmitriy L. Speransky, Vladimir V. Udut, Mikhail A. Buldakov
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This study aimed to show the possibility of regulating the metabolic capabilities of cancer cells in vitro by changing the medium composition, using the MSF-7 breast cancer cell line as an example. The relevance of the work is due to the solid necessity for constantly improve cell lines for testing new antitumor drugs and obtaining new information about the chemistry of cancer.
The assessment of glucose oxidation type by measuring the production of lactate and polarographic determination of oxygen consumption under normal conditions and the influence of Oligomycin A, 2,4 Dinitrophenol, or Rotenone action were basic methods of this study. An excess of glucose or removing glutamine was created as medium composition changes. A culture of Vero non-tumor cells was applied as a comparative model.
As a result, the lactate production by MCF-7 tumor cell culture is significantly higher than the same by Vero culture, which is consistent with the Warburg effect. Excess glucose led to decreased lactate production in MCF-7 culture by almost 10 %, but it was not observed for Vero culture. The glutamine decrease in the culture medium does not significantly affect the change in lactate production for both Vero cells and MCF-7 cells. In the culture of tumor cells, excess glucose and lack of glutamine during daily incubation did not statistically affect respiration, while these changes were accompanied by a decrease in oxygen consumption by cells by 10 % and 15 %, respectively, in the culture of Vero cells.
The obtained data can be used to control the cultivation system and verify metabolic changes due to testing new antitumor agents in vitro.