Journal of World's Poultry Research

38-42

Authors: Salah Shaban Abdelrhman1,4*., Ehab Abd-ELsabour M. Rihan 2,5, Hany M. Almotairy 3, Ali H. Al Jassem 1 and M. H. Al-Blowi1

Two hundred and sixteen samples (80 Tracheal swabs and 136 blood serum) were collected from four commercial ostrich farms in the Kingdom of Saudi Arabia for isolation of Newcastle disease (ND) and Avian Influenza (AI) viruses or detection of their antigens and antibodies by RT-PCR and Enzyme Linked Immune Sorbant Assay (ELISA) respectively. Neither AI and ND viruses nor their antigens were isolated or detected in any of the collected swabs samples. Also, Antibodies to avian influenza virus were not detected in the collected serum samples. Antibodies to ND virus were detected in 0.0%, 80.0%, 74.1%, and 80.0% in serum samples from farms 1, 2, 3 and 4, respectively. Mean ND ELISA antibody titer were 0.0, 646.3, 1068.5 and 1440.5 in farms 1, 2, 3 and 4, respectively. Ostriches vaccinated with live ND vaccine by drinking water were negative for ND antibodies, while ostriches vaccinated with live ND vaccine by spray method and boosted by inactivated oil emulsion ND vaccine gave the highest immune response. Ostriches vaccinated with inactivated oil emulsion ND vaccine (4x chicken dose/ostrich) elicited effective immune response and transferred maternal antibodies to ostrich chicks at the rate of 85.7% and 66.7% with mean ELISA antibody titer of 1450.5 and 1350.15 at 7 and 21 day old, respectively.

54-56

Authors: Nadir Alloui1* , Mohamed Nabil Alloui2, Omar. Bennoune 1 , Salah. Bouhentala1

This study was carried out during summer season on two broiler buildings with a capacity of 10000 each one. The first building with natural ventilation while the second was recently renovated with dynamic ventilation system composed of 10 ventilators with a capacity of 5000 m 3 /hour/unit. The results of this experiment showed that ammonia concentration was higher in the building with natural ventilation during various breeding phases. The average concentration of ammonia in the building with natural ventilation was already higher than standard levels from the third week of breeding (16.5 ppm) and reached higher and alarming levels at the seventh week (31.5ppm). Dynamic ventilation in the second building made possible decrease in this concentration that reach 19.5 ppm at the fourth week and 13.50 ppm at the end of breeding (week7). The variation of the ammonia levels in the building with dynamic ventilation is in close relationship to the ventilation flow and working time of the extractor fans. Mortality in the building with natural ventilation was twice higher than that with dynamic ventilation (10.5 vs. 4.5%). The presence of dynamic ventilation has also positively influenced the performance parameters of chicken. The day56, live weight of broiler chickens in the poultry house of dynamic ventilation was superior to that of broiler chickens in building of natural ventilation (1870 vs.1682 gr). This is confirmed by better feed conversion (1.98 vs. 2.66). In the building with natural ventilation, examination of 50 chickens shows the presence of conjunctivitis in animals (20%).

63-67

Authors: Aseel A. Saeed1 , Mayada F. Hasoon2 and Majed H. Mohammed3,4*

ABSTRACT This study was conducted to determine the prevalence of Salmonellae contamination of chicken meat imported from different origin to local markets in south of Iraq (Diwaniya). The bacteria were cultured, isolated and biochemically characterized by the analytical profiling index (API 20E system). The 16s rRNA and invA gene primers were selected specifically for the detection of Salmonella to amplify a 406 and 558 bp DNA fragments, respectively. The results of this study showed that 22 Salmonella isolates were detected by polymerase chain reaction (PCR) from 100 chicken meats and only 7 isolates out of 22 were identified as S. typhimurium, the highest percent of isolates were 83.8 % for India origin and the lowest percent were 25% from Jordan origin.