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Prevalence, Antibio-Resistance and Risk Factors for Salmonella in Broiler Turkey Farms in the Province of Khémisset (Morocco)
Authors: Abdellah El Allaoui
Number of views: 435
The problem of contamination of poultry by salmonella in addition to the increase of antimicrobial
resistance in Salmonella is of a great importance both in the field of public health as well as in the
socio-economic sector of the country because of the damage it can cause, but no studies have been
conducted so far in Morocco to determine the risk factors of Salmonella contamination in broiler
turkey farms. In order to determine the statute of the broiler turkey farms (n= 20) with respect to
the contamination by Salmonella, three visits were paid to each livestock buildings. A batch of 10
pools of 5 droppings per breeding by visit were collected (n= 600) and analyzed. All Salmonella
(n= 62) isolates were serotyped, confirmed by the presence of virulence gene (invasion) and tested
for the resistance to 15 antimicrobial agents by the agar diffusion method. In parallel, an analytical
study was carried out to investigate risks factors of Salmonella contamination in these farms. The
rate of insulation of Salmonella is important (35 %) and the isolated serotypes are worrying: 36 S.
Kentucky, 15 S. Saintpaul, 8 S. Parkroyal and 3 S. Ruzizi River. They have relatively high rates of
resistance to tetracycline (79 %) and streptomycin (72.5 %), followed by resistance to nalidixic
acid (37.1 %), ciprofloxacin (33.9 %), ampicillin (33.8 %), spectinomycin (32.3 %), trimethoprim
(30.6 %) to trimethoprim-sulfamethoxazole (24.2 %), gentamicin (21 %), kanamycin (17.7 %) and
amoxicillin + clavulanic acid (16.1%). Three strains of S. Agona expanded spectrum
betalactamase producing which have a high level of resistance to ceftriaxone with a minimum
inhibitory concentration (CMI) of 16 µg/ml. The variables associated with this contamination are
related to the duration of crawlspace (p = 0,037), treatment with antibiotics (p = 0.001) and the
contamination of turkeys poults (p = 0.002) dice implementation, The storage of manure inside the
livestock building (p = 0.003), The conservation of turkeys inside the rearing building (p = 0,009)
in the breeding season (p = 0.001) and age of turkeys sample (p = 0.01). The high level of
antibiotic resistance of Salmonella isolates in the present study, showed the possible Significance
of turkey as a source of multiple antimicrobial-resistant Salmonella for human infections.