Caspian Journal of Dental Research

8-15

Authors: Zahra Sadat Madani, Zinat Sadat Rezaie*, Ebrahim Zabihi, Zeinab Abedian

Introduction: Following dental avulsion, the immediate recommended treatment is tooth replantation to avoid adverse effects. Therefore, the tooth must be stored in a physiological storage medium to preserve the viability of the periodontal ligament fibroblast (PDLF) cells during transportation to dental office. The aim of this study was to determine the effect of several storage media in preserving the viability of human PDLF cells at different times and temperatures. Materials & Methods: In this experimental study, the human PDL cells were obtained from the healthy extracted third molars and cultured in Dulbecco's Modified Eagle Medium (DMEM). The studied media were DMEM (10% FBS + 1% penicillin G Na (10000 IU) + 1% streptomycin (10 mg)), tap water, sterilized whole milk, zero fat milk and soy milk. After the cells had reached sufficient density in the plate, they were added to the experimental media and kept at 1, 2, 4 and 24 hours at 4° and 37oCentigrade. After incubation, the cell viability was determined by tetrazolium salt-based colorimetric (MTT) assay. The results were statistically analyzed using Kruskal–Wallis and post hoc Tests. Results: Whole milk and DMEM showed significantly higher protective effect than other media. The viability of PDL cells had significant difference at 4oC compared to 37oC at 4 and 24 h in DMEM group and at 24 h in whole milk group (p≤0.05). Conclusion: The results have suggested that the whole milk like DMEM have enough essential nutrients for PDLFs and have confirmed the hypothesis that the milk similar to HBSS or DMEM might be effective in preserving the PDLF cells.

24-30

Authors: Zahra Hashemi * , Leila Manzouri , Fatemeh Rahimi , Maryam Farzadmoghadam , Seyed Ali Saleh Gholami

Introduction: Healthy primary teeth are necessary for a child's physical and mental health. Increasing mothers’ awareness of the importance of these teeth is significant for the creation of healthy teeth in their children's mouths. Thus, the aim of this study was to evaluate mothers’ awareness of the importance and care of their children’s primary teeth in Yasuj Material & Methods: In this cross-sectional study, 384 mothers with their children aged ≤6 years were enrolled. The data were collected via a 12-item researcher-made questionnaire and analyzed using mean, standard deviation, Spearman and binary logistic regression tests. Results: The mean age of mothers was 29.71±5.04 years. The mean score of mothers’ awareness of the importance of primary teeth was 5.9±2.76) score of 0-12).No mothers had good awareness, 119 (31%) and 265 (69%) mothers had moderate and poor awareness in this regard, respectively. A significant relationship was found between mothers’ age and awareness (p=0.0001, r=0.18) as well as between parental education level and awareness (p=0.0001, r=0.25). Parental education levels including subdiploma P=0.009, EXP (B)=15.19, 0.95% CI= 1.97-116.91; diploma and technician P=0.0001, EXP (B)=39.42, 0.95% CI= 5.03-308.81; Bachelor’s degree P=0.001, EXP (B)=27.02, 0.95% CI= 3.55-205.73; Master’s degree and higher P=0.003, EXP (B)=26.26, 0.95% CI= 2.95-233.26 as well as maternal age P=0.008, EXP (B)=1.07, 0.95% CI= 1.02-1.14 were predicting factors for mothers’ awareness of the importance and care of the primary teeth. Conclusion: Considering the undesirable level of mothers’ awareness of the importance and health, the interventions and planning are essential to enhance mothers’ awareness in this regard.

37-42

Authors: Azin Jamshidifar,Zahra Sadat Madani*

Introduction: Most of the materials used in endodontic procedures may lead to discoloration.This study compared the discolorations induced following treatment by MTA, CEM ,and Biodentine using a spectrophotometric analysis. Material & Methods: In this experimental study Forty extracted mandibular anterior teeth were selected and sectioned from 2 mm below Cemento Enamel Junction (CEJ) using a disc. A retrograde method was used for drilling cavities 2 mm away from incisal edge. Then the cavities were washed using 5.25% NaOCL and normal saline and the samples were divided randomly into 4 groups. The cavities in groups 1, 2, and 3 were filled by MTA Angleus, Cem cement, and Biodentine, respectively as deep as CEJ level and they were sealed by 2mm of A3 composite color. All of cavities of group 4/control, were all filled by composite A3. Then the samples were stored in a glass incubator with 100% humidity and under laboratory light .they were exposed to a spectrophotometric analysis within 6 periods from placement including base line, 1 week, 2 weeks, 1 month, 3 months, 6 months. The data were analyzed by SPSS 17 ,Tukey HSD and ANOVA statistical test. Results: The results showed no statistically significant difference in discoloration rate during measurement periodsin each group. However, the discoloration rate varied in various materials. The minimum and maximum discolorations occurred in Biodentine and MTA, respectively and their difference was statistically significant. (p=0.033)Other groups showed no statistically significant difference. Conclusion: The biodentinehad minimum discolorations compared with other two materials; thus, it seems that it can be recommended for cosmetic areas.

51-54

Authors: Azade Mahmuodi Kelarijani , Mohammad Mehdi Naghibi Sistani *, Shima Nafarzade

Introduction: Regarding the lack of data on the frequency of oral cancer in Babol, the aim of this study was to evaluate the frequency of oral cancer in Babol during 2012-2016. Material & Methods: This retrospective cross-sectional study was performed using pathology sheet records of oral cancer patients during 2012-2016. Survival status of patients was collected through telephone contact. Results: The mean age of 48 patients was 62.2% (22-95) years, and 64.2% were male. Squamous cell carcinoma (SCC) was the most prevalent malignancy, and the most common site was tongue followed by pharynx. Frequency of oral cancer patients was 9 (18.8%), 9 (18.8%), 10 (20.8%), 12 (25%) and 8 (16.7%) patients from 2012 to 2016, respectively. Conclusion: SCC, specifically on the tongue was the most frequent malignancy. It seems that the oral cancer prevention training is necessary for elderly adults.