Indonesian Journal of Animal and Veterinary Sciences

12 - 17

Authors: Herdis ., Kusuma ., M Surachman, I.K Sutama, M Riza, I Inounu, B Purwantara, I Arifiantini

The sperm is very fragile to lipid peroxide reaction, that it can easily broken during the process of freezing. To eliminate this consequences an antioxidant agent added into the extender. A research was done to observe the effect of antioxidant agent α-tocoferrol and butylated hydroxytoluene (BHT) presence in the extender on the quality of frozen semen. Once week, semen from six male Garut sheep ages about 2.5 years old was collected using artificial vagina and egg yolk skim-milk diluent used as the extender. The semen were treated in egg yolk skim-milk diluent without antioxidant as control, in egg yolk skim-milk diluent with α- tocoferrol 0,2 g/100 ml diluent and in egg yolk skim-milk diluent with butylated hydroxytoluene 0,2 g/100 ml diluent. The after thawing observation shown that in egg yolk skim-milk diluent with α- tocoferrol had life percentage (75.0 ± 3.5% vs 64.8 ± 7.8%) and membrane intact percentage (65.8 ± 6.8 % vs 55.2 ± 8.3%) significantly higher than control (P<0,05) but insignificantly different from with BHT addition. The presence of α-tocoferrol in the diluent, the motility percentage consideraly higher (P<0.05) than (45.8 ± 3.8%) using BHT addition (40.0 ± 4.5%) but not different from control (41.7 ± 4.1%); while acrosomal intake percentage after α-tocoferrol (54.8% ± 3.3%) expressively higher (p,0.05) than BHT addition (49.7 ± 3.6%) or control (49.8 ± 3.5%). In conclusion the presence of α-tocoferrol in the diluent could improve the quality of Garut sheep frozen semen. Key words: Antioxidant, sperm, Garut sheep

22 - 29

Authors: I-W Mathius, D Yulistiani, W Puastuti

Production respons was related to rumen undegradable protein feeding. Twenty-six local late pregnant ewes are penned individually and allocated to four dietary treatment groups of feed supplement, containing different amount of protected soybean meal and provided 0% (R1), 10% (R2) 20% (R3) and 40% (R4) crude protein of feed supplement respectively. Diets were fed until the first 8-week of lactation phase. Results showed that the mean of total dry matter intake (DMI) during late pregnancy was 3,42% of body weight (BW). Dietary treatments of protected-protein tended to increase daily mean intake of dry matter (P>0.05), ie. 76.9; 77.25; 77.49 and 78.81 g/kg BW 0.75 for R0, R1, R2 and R3 respectively. A similar trend was noted in daily intake of crude protein with the mean of 9.75; 10.31; 10,75 and 12.1 g/kg BW0.75 (for R0, R1, R2 and R3 respectively) and were not different significantly (P>0.05) among dietary treatments. Increasing the amount of protected-protein in the ration influenced (P<0.05) the daily live weight gain (ADG) of ewes during the late phase of gestation which was 104.4, 99.4, 120.8 and 148.14 for R0, R1, R2 and R3 respectively. Feed efficiency (DMI/ADG) improved as substitution of protected-protein increased. The total birth weight of lambs (g/ewe) was not significantly different (P>0.05), by increasing level of protected-protein in the ration, averaging 3.2 kg/ewe. Increasing protected-protein, increased (P<0.05) the daily gain (g/ewe) of lambs during the first 8-week of lactation, and it was 127, 131, 165 and 211 g/ewe/d for R0, R1, R2 and R3 respectively. Consequently, the weaned weight at 8- week of ages increased significantly different (P<0.05) as the amount of protected-protein in the diet increased. Key words: Banana latex, protected-protein, ewes production

38 - 45

Authors: Pius P Ketaren, L.H Prasetyo

Feed cost is the major cost for duck-egg production which accounted to be more than 70% of total production cost. Feed efficiency of duck-egg production in Indonesia is low. The main reasons for the low feed efficiency (high feed conversion ratio, FCR) are (1) genetic trait (2) high feed wastage and (3) inadequate nutrient content of diet. An experiment was conducted to evaluate effect of restricted feeding of pelleted diet on Mojosari x Alabio (MA) cross-bred duck performances from 20-43 weeks old. The experiment was designed in a Completely Randomized Design. 156 MA cross-bred ducks were devided into three groups and fed three experimental diets: (1) 70%, 85%. and ad libitum. All experimental diets were fed to ducks for 24 weeks and feed intake, egg production, egg weight, FCR, molting and egg quality: egg yolk weight, albumen wight, egg shell weight and egg yolk colour score were measured as parameters. The results showed that the restricted feeding of 70% and 85% significantly (P<0.05) reduced egg production, egg weight and induced early molting but did not adversely affect duck livability and egg quality. MA croos-bred duck was more efficient by feeding ad libitum than the restricted feeding. FCR of duck fed 70% (6.38) was significantly (P<0.05) worst compared to FCR of duck fed 85% (3.68) and ad libitum (2.88). Key words: Restricted feeding, MA crossbred duck, productivity and egg quality

55 - 61

Authors: Adin Priadi, Lily Natalia

Cross protection conferred by a live Pasteurella multocida B:3,4 vaccine to infection by P. multocida B:2, the haemorrhagis septicaemia causing bacteria in cattle was investigated. Intranasal aerogenic immunization and subcutaneous injection of the live vaccine were applied to groups I and II of 5 Bali cattle respectively. Another group (III) of 5 cattle were vaccinated with standard oli adjuvant killed vaccine intramuscularly. Cattle were observed for clinical signs and body temperatures were measured. Sera were collected monthly for 12 month and kept at -200C for further testing by ELISA. No adverse sign was observed at cattle of groups I and II after vaccination with the live vaccine. Both intranasal and subcutaneous vaccination of live vaccine showed a similar serological response which started at month-5, peaked at month-(6-7) after vaccination and still sustained at the level above positive cut-off (88 ELISA Unit) at the end of observation month-12. Cattle vaccinated with killed adjuvanted vaccine responded earlier, peaked at 5-6 month after vaccination and declined steadily till the end of investigation. At 6 and 12 months after vaccination catlle were challenged with P. multocida B:2. All vaccinated cattle challenged at 6 months (C-1) and 12 (C-2) months after vaccination survived and showed no clinical signs. Body temperatures of all vaccinated cattle were normal and ranged from 38.10C to 39.10C and 38.50C to 39.50C for cattle chalenged at C-1 and C-2 respectively. However, there was 1 cattle of group I at C-1 showed an initial increase of body temperature to 400C and decreased to normal at 42 hours after challenge. One catlle of group II had a body temperature of 40.70C detected at 5 hours post C-2 and reached a normal temperature at hour-11. Both unvaccinated cattle at C-1 and C-2 died and had body temperatures of 41.40C and 41.10C respectively at the time of death. This investigation shows that live vaccine P. multocida B:3,4 is safe and can protect cattle from haemorrhagic septicaemia for at least 12 months. This vaccine is promising to be used to replace oil adjuvanted killed bacterin for haemorrhagic secticaemia. Key words: Live aerosol vaccine, protection, haemorrhagic septicaemia, cattle