Journal of Intellectual Disability - Diagnosis and Treatment

31-36

Authors: Vadym N. Goncharenko

The pathogenetic aspects of stress on reproductive health and fertility of a woman are multi-vector, this is justified by the need for timely diagnosis and the development of new approaches to differentiated treatment of PA.Objective: to determine the characteristics of the hormonal profile, the psycho-emotional state and changes in the bioelectric activity of the brain in women with stress-induced psychogenic amenorrhea (PA).Material and Methods:The main group included 54 women with PA from 19 to 37 years, the control group – 25 healthy women from 18 to 35 years. Blood plasma FSH, LH, E2, P, Pr, DHEAS, C levels were checked by the immuno-enzymatic method, bioelectric activity measurement of the brain – with an electroencephalography, women psycho-emotional status – by the Spielberg-Hanin test, statistical data processing was performed using Windows SPSS 11.5.and was carried out by means of standard methods.Results: Patients were divided on 3 subgroups depend on significant (р<0.05) Prl or C or their combination levels increasing. The presence of nonspecific signs of activation of the structures of the brain stem in patients with PA was established by EEG with significantly (р<0.05) higher frequency. The analysis of the psycho-emotional status of women showed a high level of personal and reactive anxiety.Conclusions: The revealed features of hormonal imbalance and psycho-emotional status in women with PA indicate the necessity of involving adjacent specialists - neurologists and psychotherapists for timely and adequate examination with the appointment of differentiated pathogenetically substantiated treatment

47-52

Authors: Kamrani Solmaz, Khatere Tooba, Mohammad Zaefizadeh

Breast cancer is the most common cancer and a common cause of death, which occurs due to cancer among women in the world. Cancer cells need a lot of energy to their uncontrolled growth, so it seems that the expression of the enzyme in the Krebs cycle is changing. There are some reports about mutations and altered expression of succinate dehydrogenase, fumarate Hydratase,and isocitrate dehydrogenase in human cancers.This research aimedto investigate the role of magnetite nanoparticle Oleuropein on the Krebs cycle proteins expression on the breast cancer cell line. Oleuropeinis one of the polyphenoliccomponents in olive trees and has some benefits in some diseases,including cancer.In addition to testing the viability test MTT (3- 4,5 Dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide) assay, in three levels of Oleuropein 0ppm, 300ppm, 600ppm proteomics analysis was also performed in cell line MCF7 in this study. The results ofdifferential protein spots identification into two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS-MALDI-TOF-TOF), showed that fumarylacetoacetatehydrolase, succinate-coenzymeA ligase and isocitrate dehydrogenase1 are differential proteins upregulated after treated with 300ppm and 600ppm of oleuropein. It seems that Nano Oleuropein is a booster of Krebs cycle with upregulation of Fumarylacetoacetase, succinate-CoA ligase, and isocitrate dehydrogenase1. Uncoordinated Overexpression of some Krebs cycle protein can be one of the inhibition mechanisms on the breast cancer cell line under Oleuropein treatment