Biotechnologia Acta

7-22

Authors: T. P. Pirog, I. V. Savenko, D. A. Lutsay

The literature data of recent years about capacity of biosurfactants synthesized by bacteria (Pseudomonas, Lactobacillus, Bacillus) and fungi (Candida, Trichosporon, Saccharomyces) not only to avert the adhesion of microorganisms on the different materials, but also to destroy formed biofilms on them were presented. The perspective of biosurfactants to prevent pathogens colonization on biotic and abiotic surfaces, that is known, can be a reason of cause and spread of infectious diseases was discussed. The data of our researches about antiadhesive properties of biosurfactants synthesized by Acinetobacter calcoaceticus IMV B-7241, Nocardia vaccinni IMV B-7405 and Rhodococcus erythropolis IMV Ac-5017 were presented.

37-43

Authors: V. O. Chernyshenko, D. S. Korolova, T. V. Nikolaienk, V. Е. Dosenko, D. O. Pashevin, V. I. Kalchenko, S. O. Cherenok, N. N. Khranovska, L. V. Garmanchuk, E. V. Lugovskoy, S. V. Komisarenko

The aim of the research was to study a potential antithrombotic sodium salt of calix[4]arene-methylene-bis-phosphonic acid (С-145) — on activation and aggregation of platelets in vivo, as well as on proliferation and apoptosis of endothelial cells in the cell culture. Effects of calix[4]arene С-145 estimated in vitro after addition to the platelet rich plasma, and in vivo after intravenous injection into rabbit bloodstream in equivalent amounts (46 μM). Aggregation of platelets was induced by adenosine diphosphate and detected using aggregometer Solar AP2110. Platelet shape and cytoplasmic granularity were monitored on COULTER EPICS XL Flow Cytometer. The level of tissuetype plasminogen activator — tPA — was estimated using enzyme-linked immunosorbent assay ELISA. Effects of calix[4]arene C-145 on culture of endotelial cells cells was studied using 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide — MTT-test. The population of proliferative pool of cells (G2/M+S) was determined using flow cytometry. Aggregometry and flow cytometry showed that calix[4]arene C-145 did not activate platelets nor affect their aggregation in vitro. However intravenous injection of calix[4]arene C-145 into the bloodstream of healthy rabbits leads to strong inhibition of platelet aggregation and changes of shape and granularity of most of the platelets after 2 hours of administration. Any additional appearance of endothelial cells activation marker tPA in vivo and any inhibition of calix[4]arene C-145 on proliferation of endothelial cells in cell culture did not observe. So calix[4]arene C-145 had strong anti-platelet effect in vivo that was not a result of their direct action on platelets or endothelial cells in vitro. This allowed to assume the possibility of calix[4]arene C-145 use as an effective antithrombotic agent.

52-60

Authors: L. D. Varbanets, N. A. Nidialkova, N. V. Borzova, I. Yi. Sayfulina, O. E. Marcinko, O. A. Chebanenko

The aim of the research was to study the effect of a number of coordination compounds of stanum and germanium (compounds 1‒8) as modifiers of activity of peptidases and α-galactosidases. The coordination compounds with the same type structure of were investigated as enzymes effectors. Two types of complexes: 1) [M(H2O)6][Ge(НCitr)2]4H2O (M = Mg(1), Mn(2), Co(3), Ni(4), Zn(5)), containing biscitrate-stanate anion ([Ge(НCitr)2]2-), and 2) [M(H2O)6][Sn(НCitr)2]4H2O (M = Mg(6), Co(7), Ni(8)), containing biscitratostanate anion and various hexaaquacations ([M(H2O)6]2+, М= Mg, Mn, Co, Ni, Zn) were studied. It is shown that the compound 6 which is a biscitratostanate complex containing magnesium ions as metal, can be used for stimulation on 20‒25% of collagenase activity of B. thuringiensis var. israelensis IMV B-7465 peptidase 1 and peptidase 2. Compound 1 (biscitrate-stanate complex containing magnesium ions as metal) and 7 (biscitratostanate complex containing cobalt ions as metal) and compound 6 in a concentration of 0.001% are able to increase elastolytic activity of peptidase 1 on 55‒58%. However compound 7 has shown the greatest activating effect. It increased the elastolytic activity of peptidase 2 on 100‒140% (for both tested concentrations). This indicates that the compound 7 can further be used as of peptidase 2 elastolytic activity effector. In the study of effect of the considered coordination compounds on the activity of α-galactosidase of Penicillium canescens, Cladosporium cladosporioides and Aspergillus niger was found that when using a number of complexes (1‒2 and 4‒8), there is a slight increase on 12‒20% of enzyme activity of P. canescens, and the maximum effect (~20%, concentration 0.01%) was provided by complex 6.

69-75

Authors: O. V. Skachkova, N. M. Khranovska, L. M. Skivka, P. I. Sydor, M. V. Inomistova, T. S. Vitryk

The aim of the research was to examine the effect of Staphylococcus aureus Wood 46 cytoplasmic membranes extract on the maturation and functional activity of generated in vitro dendritic cells from monocytes in the peripheral blood of healthy patients and patients with malignancies. Found that the cytoplasmic membranes extract in the minimal of the studied concentrations (0.2 mg/ml) promoted the increased reliable expression of CD86 and HLA-DR molecules and of mRNA of cytokines ІFN-γ and TNF-α, and CC chemokine generated from CCR7 in dendritic cells. The results showed that the cytoplasmic membranes extract contributed to T1-helper polarization of dendritic cells and can be used to generate them in the dendritic cell vaccines production for cancer patients immunotherapy.