Animal Production

1-6

Authors: Olufisayo Oluwadamilare Leigh, Lukman Mustapha, Agbugba Chinyere Linda, Adaeze Ezenwanyi Ibiam

Pregnancy rates (PR) resulting from timed artificial insemination (TAI) with frozen semen were investigated in 192 Sokoto Gudali (SG) cattle following estrus synchronization under 11- and 12- day treatment intervals with prostaglandin F2 alpha (Estrumate®). Two-point inseminations at 60 h and 72 h post Estrumate® treatments were used. All cattle were randomly allocated to 2 groups i.e. A and B, such that each sub-group of A1, A2, B1 and B2 had sixteen (16) cows and thirty two (32) heifers. Furthermore, cattle in Groups A and B were administered double Estrumate® injections at 11- and 12-day intervals, respectively. Cattle in sub-groups A1 and B1 were inseminated at 60 h post second Estrumate® injection while A2 and B2 were inseminated at 72 h. Pregnancy was diagnosed using the 60-day non return to estrus and confirmed via rectal palpation at 90 days post insemination. The results showed the following PR: 33%, 83%, 33% and 33% for cattle in sub-groups A1, A2, B1 and B2, respectively. It is concluded therefore that timed artificial insemination at 72 h in SG cattle treated with double Estrumate® injections at 11-day interval produced the best pregnancy rate. These findings raises hope on the future application of TAI in breeding the SG cattle with prostaglandin F2 alpha as single agent of estrus synchronization.

17-27

Authors: Firman Febrianto, Ismoyowati Ismoyowati, Mochamad Mufti, Prayitno Prayitno, Dattadewi Purwantini

The aims of this research was to identify the qualitative and quantitative difference of the phenotypes of native mallard and Muscovy, and Muscovy the genetic diversity between Muscovy and mallard using RFLP method with growth hormone primer (GH) gene. The materials were 30-week old male and female ducks from 5 strains—Magelang, Mojosari and Tegal ducks, white-feathered, and black and white-feathered Muscovy. Muscovy Completely Randomized Design was used to analyze parameters 5 strains of ducks based on gender and with 5 replications. The parameters measured were local and Muscovy duck phenotypes and genetic diversity (number of alleles) based on GH gene, heterozygosity and genetic distance. Qualitative properties were analyzed descriptively and quantitative properties were analyzed using Anova followed by HSD in case of significant differences, whereas RFLP analysis was used to determine the allele frequencies, genotype frequencies, genetic diversity, and genetic distance of local ducks. Result showed phenotypic differences between Anas platyrhynchos and Cairina moschata. The dominant feather colorof Muscovy was black and white while mallard was brown. The bill color Muscovy Muscovy was pink with dark brown but black in mallard. The dominant shank’s color in both Muscovy and mallard was black. Body size of mallard was larger than that of Muscovy Muscovy, and Magelang ducks weigherd more than Tegal and Mojosari Duck. The PCR-RFLP results showed lower heterozygosity of mallard compared to Muscovy based on GH gene, and genetic distance of the Tegal duck was closer to Mojosari and Magelang ducks, whereas Magelang and Mojosari ducks had a considerable genetic distance based on the GH gene.

39-44

Authors: Retno Iswarin Pujaningsih, Baginda Iskandar Moeda Tampoebolon, Widiyanto Widiyanto, Dian Wahju Harjant

The use of papaya latex formulated with nitrogen supplementation and minerals in the form of multinutrition blocks that can pass through the rumen is expected to improve nutritional quality and feed digestibility. This study aims to evaluate the effectiveness of the use of fermented rice straw, papaya fruit latex and blood clamshell flour as a main raw material in the manufacture of multinutrition blocks plus herbal anthelmintic medicine (papaya fruit latex) on physical quality (texture, color, aroma, hardness), chemical quality (proximate composition and mineral content of Ca, P, Mg, Cu, Zn). Microbiological tests were carried out on total bacteria, Salmonella and Escherichia coli. The research design used was a completely randomized design with 4 treatment levels of papaya fruit administration and 4 replications. Data is processed statistically using ANOVA. The results of organoleptic physical quality analysis showed significant differences in the texture of the product given the highest papaya latex (0.007ml / kg). Chemical analysis of the product showed a quantitative increase in protein, although statistically the treatment did not show any significant differences. The results of the analysis of the microbiological quality of the product quantitatively showed that the resulting multinutrition block herbs were not contaminated with negative bacteria. It was concluded that multinutrition block herbs can be given to livestock as supplementary feed.

53-59

Authors: Diana Indrasanti, Mohandas Indradji, Sri Hastuti, Nur Fitriyani, Sitie Munawaroh, Nita Hikmawati, Desika Indra Irawan

The objective of the research was to investigate body weight, oocyte elimination and blood profile of rabbits infected with various doses of Eimeria stiedai isolates. The observed rabbits’ blood profile included erythrocyte, hemoglobin, hematocrit, leucocyte, thrombocyte, total protein plasma (TPP) and fibrinogen. Twenty-five male New Zealand White rabbits aged 3 months and weighed approximately 2 kg were provided with pellet and boiled drinking water and Eimeria stiedai isolates. The experiment used Completely Randomized Design to analyze 5 treatments with five replicates. The examined variables included D0: Infection 0 (control of infection without challenge test), D1: Infection 101 with challenge test 103, D2: infection 102 with challenge test 103, D3: infection 103 with challenge test 103, D4: infection 0 with challenge test 103 (control of infection). Data were subject to analysis of variance followed by Honestly Significant Difference Test (HSD). Analysis of Variance result showed that there was no significant difference on body weight, oocyte elimination and blood profile including erythrocyte, hemoglobin, hematocrit, leucocyte, thrombocyte, and fibrinogen. However, total protein plasma (TTP) was significantly different at 5% HSD. It can be concluded that challenge test with Eimeria stiedai has not been used as an alternative in increasing rabbits’ body immune against coccidiosis infection.

71-80

Authors: Naroso Naroso, Sriroso Satmoko, CM Sri Lestari

The purpose of research is finding out the influence of internal factors and external members on the sustainability of livestock fattening beef cattle breeding business in Central Java jepara regency.This research was conducted in February until Juni 2017 in Jepara regency. The used of research method is survey. The research material is breeder as respondent wit the total of 250 people who are taken fromtaken from 3 districts and villages stratified random sampling based on the number of cattle fire pieces in the most district, medium and at least. The equipment used here is a questionnaire (questionnaire). The data analysis used here is regression followed by path analysis.The independent variables in this research are internal factors and external factors of members. The internal factors of the members consist of: members motivation (X1), membersknowledge (X2), members attitude (X3) and external factors of members consisting of: information exposure (X4), the role of instructorrole (X5), livestock performance (X6). The dependent variable is member sustainability (Y1). The results showed that simultaneously and partially the members motivation, members knowledge, members attitude, the instructorrole and livestock performance affect significantly on the groupssustainability variable

143-150

Authors: Didid Diapari, Widya Hermana, Febrina Prameswari, Anuraga Jayanegara

This study was aimed to examine the effect of black tea extract addition in diet containing 4% and 6% sunflower oil on ewe physiological responses and blood profiles from late pregnancy until early lactation. This study was designed using a 2×2 factorial completely randomized design with 5 replications on 20 late-pregnant ewes. The first factor was two levels of sunflower seed oil (4% and 6%) and the second factor was the levels of black tea extract addition (0 ppm and 500 ppm). Data were analyzed by using analysis of variance (ANOVA) and Duncan’s multiple range test. Results showed that supplementation of sunflower seed oil and black tea extract had no effect on physiological responses and blood profiles of ewe during late pregnancy until early lactation except for erythrocyte numbers. Erythrocyte numbers were higher on 6% sunflower seed oil supplementation than that of 4% (P<0.05). In conclusion, addition of black tea extract in diets containing 4% and 6% sunflower seed oil limitedly affect physiological responses and blood profiles of ewe.

160-166

Authors: Lalu Ahmad Zaenuri, Lukman Lukman, Oscar Yanuarianto, I Wayan Lanus Sumadiasa, Rodiah Rodiah

A study was designed to determine Kacang buck sperm membrane integrity and acrosome reaction as to the effect of different concentration of freeze drying fig fruit filtrate in tris egg yolk based extender. This study used 5 proven fertility Kacang goats aged 3-4 years, maintained by Faculty of Animal Sciences, Mataram University, Indonesia. Semen was collected by artificial vagina at every five days. The collected semen was divided into four aliquots in accordance to the treatments extender such as Control (0 gr), T1 (0.02 gr), T2 (0.04 gr) and T3 (0.06 gr) freeze drying fig fruit filtrate in tris egg yolk based extender (gr/v), respectively. Plasma membrane integrity and intact acrosome after re-concentration and preserved at 5ºC were assessed visually at 0 and every 24 hours for 5 consecutive days. The statistical significance of the result was evaluated by a one way ANOVA for completely randomized design analysis of variance. Data were presented as Mean±SD. Results suggest that the mean percentages of sperm membrane integrity in T0, T1,T2 and T3 at 96 h post extended and preserve at 5ºC were 34.3±5.3, 40.6±4.7, 44.8±5.4 and 42.1±5,1, respectively. The mean percentages of sperm acrosome intact were 16.4±4.8, 18.5±1.9, 21.6±3.1 and 19.6±2.8, respectively. The results of the study suggested that additional 0.04gr freeze drying fig fruit filtrate into tris egg yolk based extender have a significant preservation effect on both spermatozoa membrane integrity and acrosome intact of kacang buck.

179-188

Authors: Elly Tugiyanti, Emmy Susanti

The purpose of this research was to know the effect of breadfruit leaves flour in feed to blood profile and correlation between cholesterol, HDL and LDL of blood with meat cholesterol of maleTegal duck of 10 weeks old. The material used was Day Old Duck of male Tegal duck as much as 120 heads with experimental research methods and Completely Randomized Design (CRD). There were 6 treatments: feed without breadfruit leaf powder, feed + breadfruit flour 3% / kg feed, feed + breadfruit flour 6% / kg feed, feed + breadfruit flour 9% / kg feed, feed + flour breadfruit 12% / kg feed, and feed + breadfruit flour 15% / kg feed. Each treatment was repeated 5 times and each replication consisted of 4 ducks. The variables observed were the number of red blood cells, white blood cells, HDL, LDL, blood triglycerides, correlation between cholesterol, HDL and LDL blood with meat cholesterol of male Tegal duck of 10 weeks old. The result of the analysis showed that the supplementation of breadfruit leaves has significant effect (P <0,05) on the number of red blood cells, white blood cells, HDL, and blood triglyceride. There is a positive correlation between blood cholesterol with duck meat cholesterol which given breadfruit leaf powder. This research can be concluded that the supplementation of breadfruit leaf powder (Artocarpus altilis) can increase the number of red blood cells, white blood cells, blood HDL level but lowers blood cholesterol and triglyceride blood of male Tegal duck of 10 weeks old. Cholesterol meat is affected by blood cholesterol with regression coefficient of 0.780941, influenced by LDL (r = 0.407849), but negatively affected by HDL (r = -0.47022)

197-205

Authors: Triana Setyawardani, Mardiati Sulistyowati, Kusuma Widayaka, Agustinus Hantoro Djoko Rahardjo

The research is conducted aiming at analyzing the physical, chemical, and sensory qualities of goat cheese with different temperatures and storage duration. The research materials are goat milk and L. plantarum TW14 and L rhamnosus TW2 isolates, and its equipments include a set of cheese production tools, colorimeter, and SEM. The variables analyzed are cheese’s physical quality (color and structure), chemical qualities (total titrated lactic acid) and sensory qualities (texture, flavor, aroma and likeness). The treatment consists of 2 factors, namely the first factor including: storage temperature (freezer and refrigerator) and the second factor: storage duration (0; 15; 30; 45 and 60 days) with 3 replicates. The obtained results indicate that storage temperature does not influence the cheese’s brightness level (L*), a* value (redness) and b* value (yellowness) and sensory qualities (texture, flavor, aroma and likeness), yet storage duration influences the cheese’s L* value (P<0.01). Storage temperature has significant influence (P<0.01) on the cheese’s lactic acid content, while storage duration has insignificant influence (P>0.05) on it. The cheese’s structure shows protein aggregate, void and lactic acid bacteria. The conclusion is that frozen-stored goat cheese still has good physical, chemical, and sensory qualities.

13-19

Authors: Mohammad Hasil Tamzil, Budi Indarsih

This study was designed to determine the phenotype characteristics of Sasak ducks in Lombok Island. The study used 64 Sasak ducks at production stage which were kept intensively by small holder farmers. Observations on the characteristics of the feather color were carried out directly on each animal. The data observed were feather colors, beak color, and the color of the foot and shank, while the data on the size of the body that was observed were body weight, beak length, neck length, back length, breast circumference, back length, thigh length, shank length, and the distance between the two pubic bones. The data obtained were tabulated and analyzed descriptively. The study concluded that the characteristic of the color of feathers in Sasak ducks were dominated by Roman color (50.13%), followed by Tanak color (21.87%), Tongkak color (15.63%), Rombak color (6.25%), and Cemaning color (3.12%). Sasak ducks with medium size were observed to have a relatively long beak, medium-long neck, short back, broad and deep chest, medium height, and the distance between the two pubic bones was quite wide. These characteristics indicate that Sasak ducks are the type of superior Indian Runner having better production ability compared to the other types of Indian Runner duck.

81-92

Authors: Muhammad Muhsinin, Niken Ulupi, Asep Gunawan, I Wayan Teguh Wibawan, Cece Sumantri PDF

The objectives of this study were to identify polymorphism of transforming growth factor β2 (TGF-β2) gene associated with Salmonella pullorum resistance in Indonesian chickens. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assays were used to genotype Indonesian chickens. Analysis of polymorphism was conducted by using PopGen 3.2 software. The effect of genotype on S. pullorum resistance was analyzed using the SAS General Linear Model (GLM) procedure. Genotyping was performed on 278 chickens from 7 Indonesian chicken populations (Sentul, Merawang, Pelung, Kampung, Parent Cobb broiler, The F1 crossbreed of Kampung x Parent Cobb broiler (KB) and F2 KB x KB. The product of amplification was 284 bp. The TGF-β2| RsaI locus was polymorphic in all populations, producing two alleles (T and C) and three genotypes (TT, CT, and CC). The result from the analysis of the allele and genotype frequency showed that the T allele had a higher frequency than the C allele in all populations. The χ2 analysis showed that the 6 chicken populations were deviated from Hardy-Weinberg equilibrium, with the exception of the Parent Cobb broiler chicken. The association result showed that TT genotype was significantly associated with S. pullorum resistance in Sentul chicken. Although the leukocyte concentration, leukocyte differentiation and H/L ratio in Sentul chicken with three of TGF-β2 genotypes (TT, TC, and CC) were not statistically different. In conclusion, polymorphism in the TGF-β2 chicken gene can be used as a candidate marker to increase S. pullorum immune response.

101-110

Authors: Sangle Y Randa, Marlyn N Lekitoo, Deny A Iyai, Freddy Pattiselanno

This paper aimed to evaluate the nutritive value and the quality of ensiled Napier grass (Pennisetum purpureum Schum) and banana (Musa acuminate) peelings. Different levels of banana peeling and Napier grass were used in this study. The levels of Napier grass and banana peeling compared as treatments with four replications were as follows: 100% Napier Grass (NG), 75% (NG) + 25% Banana Peeling (BP), 50%NG + 50%BP, 25%NG + 75% BP and 100%BP. Napier grass and banana peeling were mixed based on the percentage combination as treatments, weighed and placed into empty jam bottle with weights ranging from 64.4 grams to 509.2 grams as a simulated laboratory silo. Samples were stored in eight weeks at the Dairy Training Research Institute. Proximate analyses were done at Animal Nutrition Laboratory Institute of Animal Science while ADF, NDF, IVDMD, and IVOMD were done at the Animal Nutrition Division Laboratory at Dairy Training and Research Institute, UP Los Baños. Physically, the color of silage were light yellow with a little greenish color for 100% napier grass, light to moderately yellow for the three combinations of napier and banana peel and yellow for 100% banana peel. Texture ranged from dry and coarse for napier grass, relatively dry for the combinations and moderately wet for the banana peel. The napier grass had a slightly acidic smell, the combinations had slightly sweet, acidic smell while the 100% banana peel had a sweeter, acidic smell. Likewise, pH before ensiling had ranged from 5.7 (25%NG + 75%BP) to 7.5 ( 100% BP) while after ensiling the pH changed from 4.575 ( 100% napier grass) to 5.75 ( 100% banana peel ). There were significant differences on DM, Moisture, CP, EE, CF, and Ash before ensiling. Similar trend was observed after ensiling except the ash content of all the treatments. ADF and NDF did not show significant variations in all treatments. IVDMD and IVOMD before ensiling varied significantly, but after ensiling only IVDMD of the treatments differed significantly. In terms of physical attributes, nutritive value and digestibility, banana peel can be used as silage material. All treatments generated comparable acceptability when fed to the animal. It is suggested that further study, specifically in vivo trial, will be conducted to validate the feeding and nutritional value of banana peel as alternative silage for ruminants.

119-126

Authors: Efka Aris Rimbawanto, Sri Suhermiyati, Bambang Hartoyo

The objectives of the research were to supply nitrogen for protein synthesis of ruminal microbe from slow release urea and to supply post-rumen protein from soybean meal protected with condensed tannin (CT) from crude Leucaena leaves extract. In Experiment 1, slow release urea (SRU) was made by extrusion of cassava waste-urea, tapioca meal-urea and cassava meal-urea. Evaluation of SRU properties was based on residual nitrogen concentration and ruminal fermentation products (total volatile fatty acid and ammonia-nitrogen) in vitro. In Experiment 2, soybean meal protected with CT from crude Leucaena leaves extract as much as 1,68 g tannin/100 g DM soybean meal. SRU that was selected from Experiment 1 was used as the supplement in sheep ration whose protein source was protected with CT. SRU supplement in basal rations was 0, 6.36, 12.75, 19.11% DM. In vitro result in Experiment 1, showed that SRU of cassava waste, tapioca meal and cassava meal were different (P<0,01) on residual nitrogen content, total VFA, ammonia-N, and fermentation time. The optimum SRU obtained from cassava waste by inhibiting nitrogen hydrolysis up to 14 h. In Experiment 2, SRU supplementation of cassava waste on basal ration whose protein source was protected with CT increased dry matter (DM) degradability (P<0,01), total VFA (P<0,01), ammonia-N (P<0,01), and did not affect crude protein (CP) ruminal degradation. Post-ruminal degradability of DM and CP increased (P<0.01) in line with the increasing supplement, and reached the optimum level at 12,75 – 19,11% DM. In conclusion, cassava waste was a potent SRU through extrusion process and could be harnessed as SRU supplement in ration with protein source protected with condensed tannin to improve ruminal microbe protein synthesis.

134-142

Authors: Krismiwati Muatip, Titin Widiyastuti, Nunung Noor Hidayat, Hermin Purwaningsih, Eko Purwanto, Ghanis Ginanjar Setya

Kebumen district is one of beef development centers in Central Java that requires numerous forage supplies (HPT). Farmers without personal foraging area have to buy forages almost on a daily basis. Forage business is an emerging and prospective business sector to improve farmers’ welfare particularly the income. Government is therefore expected to support in terms of facility and infrastructure to maintain a sustainable business practice. The aim of this study was to investigate the necessity of forage business in cattle development center in Kebumen district, forage marketing channel, forage business revenue, profit margin, R/C ratio and government support in terms of infrastructure or policy related to forage business.

1-12

Authors: Basengere Ayagirwe, Felix Meutchieye, Appolinaire Djikeng, Robert Skilton, Sarah Osama, Yacouba Manjeli

Although domestic cavies are widely used in sub-Saharan Africa as a source of meat and income, there are only a few studies of their population structure and genetic relatedness. This seminal study was designed with the main objective to assess the genetic diversity and determine the population structure of cavy populations from Cameroon to guide the development of a cavy improvement program. Sixteen microsatellite markers were used to genotype 109 individuals from five cavy populations (Wouri, Moungo and Nkongsamba in the Littoral region, and Mémé and Fako in the Southwest region of Cameroon). Twelve markers worked in the five populations with a total of 17 alleles identified, with a range of 2.9 to 4.0 alleles per locus. Observed heterozygosity (from 0.022 to 0.277) among populations was lower than expected heterozygosity (from 0.42 to 0.54). Inbreeding rates between individuals of the populations and between individuals in each population were 59.3% and 57.2%, respectively, against a moderate differentiation rate of 4.9%. All the tested loci deviated from Hardy-Weinberg equilibrium, except for locus 3. Genetic distances between populations were small (from 0.008 to 0.277), with a high rate of variability among individuals within each population (54.4%). Three distinct genetic groups were structured. This study has shown that microsatellites are useful for the genetic characterization of cavy populations in Cameroon and that the populations investigated have sufficient genetic diversity that can be used to be deployed as a basis for weight, prolificacy and disease resistance improvement. The genetic of diversity in Southern Cameroon is wide and constitute an opportunity for cavy breeding program.

21-27

Authors: Siti Darodjah Rasad, Rangga Setiawan

Aim of this study was to examine the characteristics cytology of mucous cell-,temperature- and pH vagina during estrous cycle in local sheep. 31local sheep were synchronized with vaginal sponge consist of 20 mg progesterone hormone before carried out observations of cytology of cells from the vaginal mucose through vaginal swabs, temperature and pH of the vagina. Vaginal swabs were collected daily at 7 am for a weeks.Vaginal temperature and pH measurement is carried out twice a day, at 07.00 am and 15.00 pm for a weeks after vaginal swabs. Smears of the swab were then prepared on glass slide and they were stained with Giemsa. Vaginal epithelial cells; Parabasal, intermediate and superficial cells were counted and their percentages during pro-estrous, estrous and di-estrous were determined. Di-estrous was characterized by the absent of superficial cells in the epithelial vagina. Pro-estrous was characterized by the increasing progressively of intermediate/superficial cells in epithelial vagina, whereas estrous was characterized by the presence of superficial/cornification cells in most epithelial vagina. Based on the dominance of superficial cell, the number of sheep identified as estrous is highest on third day, with 52%. Observation on vaginal temperature also resulting that the highest temperature values obtained on the third day of 39,08±0.28°C. It could be effected of the vaginal pH during the observation. Underthe influence ofestrogen, the epithelial vaginalcellssynthesizeand accumulateglycogenin large quantitiesdepositedin the lumen ofvagina. Vaginal bacteriametabolizethe glycogenformlactic acid, which causesvaginal pHis low.The pH conditions prevent from pathogenic microorganisms and fungi. Increased estrogenal so cause cell proliferation through the thickening of the epithelium lining of the vagina so that the cells differentiate.Increasing of glycogenin the superficial cells, and ceratin cells found in the cytoplasm of cells, as well as acidic pH in the lumen vagina causing changes in the chemical composition of each cell (Zaid, 2011).It can be concluded that during estrous phase, cytology of vaginal mucose cell is dominated by superficial cell and vaginal temperature is increasing as well as pH of vagina.

37-45

Authors: Dwi Margi Suci, Zurrahmi Fitria, Rita Mutia

Fat, cholesterol and fatty acid profile of breast and thigh meat of 12 Muscovy ducks (6 drakes) aged 8 weeks from the local farm in Bogor, West Java were analyzed in thus study. In general, Muscovy ducks were fed with rice bran twice a day and free-ranged in the backyard. The nutrition of rice bran is varied in crude protein, crude fiber, fat, and ash content. Muscovy ducks were slaughtered and the percentage of carcass weight, fat, cholesterol and fatty acid profile of the breast and thigh meat were measured and analyzed. The result showed that the breast meat had higher (P<0.05) ω3 total fatty acid EPA (C20:5ω3) but DHA (C22:6ω3) and linolenic acid (C18:3ω3) were not significant compared to that of thigh meat. Concentration of linolenic acid in breast meat ranged from 0.26 to 0.51% of fat and 0.35 to 0.39% of fat in thigh meat. Linoleic acid (C18:2ω6) in breast and thigh meat was 7.64 - 10.73% and 5.17 - 13.93 % fat, respectively. DHA concentration ranged from 0.13-1.31% fat in breast meat and EPA in thigh meat was approximately 0.08-0.32 % fat. EPA was significantly higher (P<0.05) in breast meat (0.03-0.28 %) than thigh meat (0.05-0.08 %) The study produced ω 6:3 ratio ranged 6.50-24.14 and 8.94-31.64 in breast and thigh meat, respectively.

55-60

Authors: Irma Badarina, Heru Dwi Putranto, Endang Sulistyowati

The objective of this research was to evaluate the anthelmintic efficacy of the ethanolic and aqueous extract of coffee husk fermented by Pleurotus ostreatus against Ascaridia galli. In vitro study of anthelmintic activity was conducted by counting the number of paralyzed worm body of Ascaridia galli during 24 hours in petri dish containing different levels of ethanolic and aqueous extract of fermented coffee husk, namely 0%, 1%, 2%, 3% and 4% (w/v) and compared to piperazine 0,5% (w/v) and NaCl 0,9%. The powdered samples of fermented coffee husk were macerated in the 30% ethanol solution with the ratio samples to solution = 1:3. In aqueous extract, samples to solution ratio was 1 : 7. The extract of fermented coffee husk possess phytochemical compunds which responsible for anthelmintic activity. The anthelmintic activity of ethanolic extract was better than aqueous extract. Fermented coffee husk ethanolic extract exhibited mortality of A.galli at concentration 2% while showed highest mortality at concentration 4%. Fermented coffee husk ethanolic extract have the potential to aid in the control of gastro intestinal parasites in animal

125-130

Authors: Veronica Margareta Ani Nurgiartiningsih, Agus Budiarto, Kusmartono Kusmartono, Suyadi Suyadi

This research was conducted to evaluate the performance of female Madura cattle in three districts of Madura Island (Sampang, Pamekasan, and Sumenep), known as breeding areas for purebred Madura cattle. Vital statistics of 1,040 female Madura cattle at 24 and 30 months old in Sampang, Pamekasan, and Sumenep district were measured and analysed. Mean of Body weight, chest girth, body length, and wither height of female Madura cattle at 24 months old were 191.7±17.6 kg, 133±3.0 cm, 124.0±2.1 cm, 115.7±2.9 cm, respectively, and those at 30 months old were 209.4±23.6 kg, 138.2±4.5 cm, 125.7±2.6 cm, 116.7±3.4 cm, respectively. The body weight, chest girth, body length of female Madura cattle at 24 and 30 months old in Pamekasan district (216.6±26.5 kg, 135.2±6.1 cm, 125.4±3.9 cm, and 238.0±28.5 kg, 141.5±6.7 cm, 127.9±3.5 cm, respectively) showed the highest value compared to those in Sampang and Sumenep district. Phenotypic correlation between vital statistics and body weight at 24 and 30 months old ranged between -0.43 to 0.90. Chest girth showed the positive highest correlation with body weight of female Madura cattle at 24 months old (0.24) and at 30 month old (0.90). It indicated that chest girth could be used as estimation parameter for body weight in female Madura cattle.

141-148

Authors: Titin Widiyastuti, Tri Rahardjo Sutardi

The purpose of this study is to assess the optimization of fermentation process by adding a minerals and amino acids so that the potential of protein of Concentrate Protein-Jatropha seed cake (CP-JSC) can be optimally used as a substitute for soybean meal. The method used was completely randomized design. The treatment consisted of F1: Fermentation CP-BBJ + methionine-lysine (0.25%: 0.25%), F2: Fermentation CP-JSC + methionine-lysine (0.5%: 0.5%), F3: F1 + 0.45% Dicalsium Phosphate, F4: F2 + 0.45% Dicalsium Phosphate. Each treatment was repeated four times, When treatment significantly continued by Least Significant Difference (LSD), variables observed are the levels of antinutrients (phorbolester, antitrypsin), the levels of nutrients (fat, protein, crude fiber, Ca, P and gross energy) and amino acid. Results of analysis of variance showed that the addition of amino acids and minerals Ca, P in the fermentation process was highly significant effect on the levels of crude fiber and phosphorus (P <0.01) and significantly affected the gross energy content of CP-JSC post-fermentation (P <0.05). Dry matter, crude protein, crude lipid and calcium are not affected by supplementation of methionine and lysine as well as calcium and phosphorus. Supplementation of methionine and lysine in the fermentation substrate showed good levels of essential amino acids and non essential higher than previous studies although not statistically significant (P> 0.05). While the levels obtained phorbolester range of 0.055% - 0.08%. It was concluded that the optimization of fermentation can be done without adding the amino acid supplementation of minerals calcium and phosphorus. Supplementation significantly affect a significant increase or decrease in some nutrients (crude fiber, gross energy, phosphor) and capable of suppressing a decrease in amino acids. Supplementation of amino acids Lysine and Methionin 0.05% is the best treatment.

157-164

Authors: Dian Ratnawati, Aryogi Aryogi

Palm oil waste (by product) can be used as a potential feed for livestock. Nevertheless, the study research of the effect of Palm oil waste (by product) as a feed to the bull performance was limited. The purpose of this research is to get technology to improve semen quality through improving protein of feed based on palm oil waste (by products). This research was conducted in PTPN 6 Jambi and used 30 bulls that separated into 3 treatments, treatment I (feed protein 12% and supplementation), treatment II (feed protein 12%) and treatment III (existing feed, feed protein 10%). Parameter were measured i.e sperm quality (sperm motility, mass movement, sperm concentration, percentage of live sperm, sperm abnormalities), feed consumption, body condition score (BCS) and average daily gain (ADG). Design of this research was completely randomized design. Data was analyzed use ANOVA. The result showed that there is no significantly different on semen quality between treatments. Semen quality of three treatments were appropriate to standard of quality semen of bull (sperm abnormality <20%, sperm motility >50% and sperm concentration >500 million/ml). It can be concluded that dietary protein level of 10% and 12% based on palm oil by product can maintain semen quality of bulls. Palm oil by product can be a potential feed to increase productivity and reproduction performance.

173-179

Authors: Lilik Eka Radiati, Firman Jaya, Heldy Oktavia

Carrot juice and milk were blended and fermented by culture bacteria Streptococcus thermophillus and Lactobacillus bulgaricus. Carrot juice affect significantly on lactic acid content (1.09± 0.12% – 1.15± 0.01%), pH value (3.80±0.06 – 4.17± 0.10), viscosity (133±2.30 cP–146±2.10cP), β-carotene (0-173.19±1.02 µg/g), EPS (11.90 ±0.50 - 18.00 ±0.40 mg/100g), β-D-galactosidase activity (2.27±0.30-192.40±0.48 µ/g) yogurt, but did not affect significantly on bacteria number (9.0± 0.5 – 9.8±0.4 log CFU/g). Carrot juice increased the yogurt culture activity with increasing acidifying, β-catotene, EPS and β-D-galactosidase, suggesting that yogurt could be fortified with carrot juice.