Studia Universitatis Moldaviae. Stiinte reale si ale naturii

3-9

Authors: Aurelia CRIVOI, Iurie BACALOV, Elena CHIRIŢA, Iulian PARA, Ilona POZDNEACOVA, Adriana DRUŢA

Bee products are a rich source of calories and an excellent source of nutrients containing a rich variety of vitamins. It also provides or complements mechanisms that naturally occur in an organism. Taking into account the benefits of bee products, they are consumed with great confidence in food.

15-18

Authors: Lidia COJOCARI, Aurelia CRIVOI, Ana TABAC

This article analyzes the causes of stress in pre-school children and its annihilation ways. Eventually , the children may have to be morally prepared for stressful situations because we cannot completely protect them from any negative situations and responsibilities.

24-29

Authors: Valentina BULIMAGA, Liliana ZOSIM, Maria PISOVA, Veaceslav REVA

Selenium-containing (Se-EPS) and EPS from Spirulina platensis cyanobacterium grown on the nutritive medium with and without selenite supplementation were obtained. The partially purified extracts (after removal of the proteins fraction) were subjected to HPLC separation on the Toyo-Pearl HW40F column. The UV spectra of the exopoly¬sac-charide preparations have been scanned and the technological scheme for obtaining of Se-EPZ produced by Spirulina platensis has been developed.

35-40

Authors: Petru CUZA

Was studied the specific distribution of the descendants of common oak (Quercus robur) by category of heights in autumn and spring sowing. It has been demonstrated that at the individual growing stage of the saplings, in the absence of competition between copies, the distribution of oak numbers by height classes, both in autumn and spring crops, is approaching the law of normal distribution. After realizing the massive state of the oak frequency curve in relation to the height, it has a right asymmetry. This demonstrates that in the competition for solar energy and mineral elements in the soil, a part of the genotypes, with superior hereditary attributes, growing fast inside tend to occupy dominant positions, embarrassing the growth of the least developed. In this way natural variability is reduced caused mainly by genetic diversity, as a result of the growing influence increasingly pronounced of environmental factors, especially trophic ones (mineral nutrition and illumination) on growth in oak height.

47-51

Authors: Алла ГЛАДКАЯ

The R. rhaponticum L root extract exhibits fungicidal and activator properties in the control of Sphaerotheca fuliginea on the family Cucurbitacea seedlings. The addition of microelements can make the composition more profitable. The combination of an extract R with a microelement Cu2 + increases the medical properties, and with microelements (Zn2+, Fe2+, Mg2+, B3+) enhances the prophylactic properties of the preparations.

57-60

Authors: Елена ПЕЛЯХ, Василий ЧОБАНУ

New hybrids of mint were obtained as a result of distinct hybridization and complicated selection in Laboratory of Plant Biochemistry of Moldavian State University. In crossing were involved species, ecotypes, chemical rases and natural populations from different geographical regions as a parental forms. The character of divergence depend on the origin of hybrids and on their genotypical specificities. Comparative studies of strains of mint revealed advantage some of hybrid thanks to high yield of oil, great amount of carvon, linalool, mentol etc.

68-77

Authors: Maria GONȚA, Vera MATVEEVICI, Viorica IAMBARȚEV, Larisa MOCANU, Gheorghe DUCA

It has been studied the process of diminishing the concentration of emollient 2,2-dihydroxymethyl proponic acid (DMPA) in the presence of the active orange textile dye (AP). It has been found that the effect of removing the organic components of the dye and thickeners mixture is increased by applying the electrofloating or electrofloatocoagulation method and the removal effect decreases when is applied the coagulation method. Also, it was found that the simulated systems which are low concentrations of dye AP (100,0 mg/L) and the con-cen¬tra¬tions of the thickener are up 20,0 to 80,0 mg/L) shall be treated by the combination of the electrofloatocoagulation and adsorption methods. If it is increasing the concentration of organic compounds, they are purified by a combination of electrofloatocoagulation and oxidation methods.

86-92

Authors: Iolanta BALAN

The Pseudo Jahn-Teller Effect (PJTE) is used to rationalize structural changes in the redox processes of NH3 and CH3 molecules by means of analyzing the changes in their symmetry and energy gaps between the ground and lowest excited electronic states that control their PJTE coupling along the distortion coordinates. The numerical values of the vibronic coupling constants were estimated by means of fitting the solutions of the secular equations to the ab initio calculated energy profiles. It is shown that oxidation of NH3 molecule by removing an electron from the high occupied molecular orbital (HOMO) leads to the suppression of the PJTE and to restoration of planar nuclear configuration. Reduction of CH3 molecule enhances the PJTE, leading to its pyramidalization.

97-106

Authors: Vasile RUSU

It was performed analysis of the characteristics of the electric double layer (EDL) close to the basal surface and edge surface of H-montmorillonite and pillared Al-montmorillonite. The thickness of compact layer (Stern layer) was esti¬mated on the basis of ions dimensions proposed by Marcus. For H-montmorillonite, the dielectric constant for Stern layer is about rI ≈ 7,2 for EDL in the proximity of the basal surface and about rI ≈ 8,5 for EDL in the proximity of the edge surface, while the surface potential 0 for these surfaces is about -0,083 ÷ -0,084 V. For pillared Al-montmorillonite, the corresponding dielectric constants for these surfaces are rI ≈ 9 and rI ≈ 12, and the the surface potential -0,121 ÷ -0,117 V in accordance with values established from Gouy-Chapman model. The electrical capacity of compact layer was estimated on the basis of established characteristics (δ, rI) and was established the increasing of electrical capacity as the thickness of compact layer is decreasing.

116-119

Authors: Vladislav BLONSCHI, Viorica GLADCHI

This paper contain the results of multi-annual study on the optimization of inhibition capacity determination, obtained after statistical processing of empirical data from 2008-2017. Thereafter of statistical processing of multi-annual data, it was proposed the average value of fading speed of PNDMA indicator in distilled water, that optimize computation of the inhibition capacity of waters.

3-7

Authors: Iurie BACALOV, Aurelia CRIVOI, Elena CHIRIȚA, Ana ILIEŞ, Iulian PARA, Adriana DRUȚA

Diabetes occurs due to insufficient insulin production by the pancreas, so the blood glucose level exceeds normal levels. Statistics show a permanent increase in diabetes cases. About 5% of the population (with some variation from one country to another) suffers from the clinical form of this disease, about 10% - of its subclinical form. Diabetes causes a number of functional disorders, as well as on gonads, that can lead to serious complications such as menopause in women and impotence in men.

14-18

Authors: Диана БАЛАН, Штефан ЦУРКАНУ, Аурелиан ГУЛЯ, Мария БЫРКЭ

There was studied the influence preparation cobalt on some biochemical blood indices of piglets in early postnatal ontogenesis. As a result of the studies was revealed that using the acid dibromanelinbisdi¬metilglioxima¬toco¬balt (III) has positively influences on some indices of the iron metabolism.

22-28

Authors: Mariana BUTNARU

The article exposed some descriptions and problems related to combating mental and behavioral disorders in epilepsy (TPCE), structure and operating principle of intelligent system to support these diseases. A module system developed by the author is the expert system for diagnosing TPCE. As a result, the system observes one of 24 diagnoses of TPCE according to the World Health Organization classifier ICD-10. The intelligent system is designed for diagnosis, for training and improvement of the health professionals.

37-40

Authors: Ala CHERDIVARĂ, Angela RUDACOVA, Serghei RUDACOV, Andrei ŞUTOV

Papain proteolysis of the storage 11S globulin Ara h3 from peanut seeds starts from the detachment of an extended -chain C-terminal sequence covering the region of -helices. Further cleavage of a loop between -strands E’ and F’ inside the central part of -chain -barrel occurs generating fragments connected with intact -chains via a disulfide bond and those retained inside the molecule of partially hydrolyzed 11S globulin due to non-covalent interactions. In accordance with the described proteolysis scenario, the C-terminal -chain region containing three of the four antigen determinants (IgE epitopes) identified in the peanut 11S globulin is destroyed during the initial papain action. Therefore, the level of Ara h3 allergenicity can be decreased via papain limited proteolysis.

46-50

Authors: Valentina BULIMAGA, Maria PISOVA, Liliana ZOSIM

Two-stage cultivation of cyanobacterium Spirulina platensis CNM CB-02 with NaCl (0,25 and 0,50 M) supple-men¬tation on the second stage of cultivation, without varying of the lighting regime ensured an increasing of the content of sulfated polysaccharides in biomass by 29 and 31,3%, respectively, compared to the reference sample (without NaCl). It wasn’t found an evident effect of NaCl on the total content of acidic polysaccharides in this case. Light intensity increasing (up to 5500 lx) with NaCl (0,25 M) supplementation ensured the increasing of the acidic polysaccharides content with 23,45%, compared to the reference sample (cultivated at 3500lx). CuSO4•5H2O (1-4 mg/l) hadn’t a significant effect on the content of acidic and sulfated polysaccharides in spirulina biomass, but considerably stimulated acidic exopoly¬saccharides production (up to 238,3 g / kg) at 5500 lx.

57-60

Authors: Sergiu DOBROJAN, Victor ŞALARU, Irina STRATULAT, Galina DOBROJAN, Evghenii SEMENIUC

Thanks to the biological fixation properties of atmospheric nitrogen and other physiological characteristics, the blue-green algae have a great potential to stimulate the productivity of crop plants having main premises for intensive applying in ecological farming. Algal bio-fertilizers administration contributes to stimulating growth at the stem height of tomato plants, increasing the number of seedling of tomatoes from 1.3 to 1.62 times and productivity tomatoes from 1.31 to 1.37 times compared to the control group where algal biomass was not given.

65-68

Authors: Iulian MAMAI

The main objective of the research constitutes the identification of the invasive (sinanthropi) flora within the reservation which has installed itself as a result of recent climatic changes or has been introduced accidentally as a result of the human activity. The invasive flora of the “Padurea Domneasca” Natural Reservation was researched during the vegetation period of the 2008-2016 years and it comprises 96 taxons, combined into 76 genii and 29 families.

74-82

Authors: Mihail MOROZ

It was studied action of remedies Catosal and Apifitostimulin-25% on nonspecific resistance of sheep and their offspring. The results showed that double administration, with 14 days, of studied remedies of sheep in last period of gestation, acted positively on nonspecific resistance in sheep and their offspring. The highest results were obtained in animal injected with Apifitostimulin-25%.

89-95

Authors: Tatiana ERHAN, Olga GARBUZ, Aurelian GULEA

The present paper describes the synthesis of 6 new thiosemicarbazides and 6 thioureas. The identity and purity of obtained organic compounds was proved by chromatography TSS, melting points, as well as by 13C and 1H NMR, IR spectra. The synthesized substances were tested, in vitro for the antiproliferative activity on cancer and normal cells of: HeLa (cervical cancer) cells, BxPc-3 (pancreatic cancer) cells, RD (rhabdomyosarcoma) cells and on normal MDCK cells. Structure-activity relationships were carried out.

108-117

Authors: Vasile RUSU

The relationship potential - the distance from the surface of the solid towards the solution by the Gouy-Chapman model, the Stern model, and the modified Gouy-Chapman model were analyzed. Modeling performed according to the Stern model for H-montmorillonite and pillared Al-montmorillonite highlights the peculiarities of the double electric layer close to the basal and edge surfaces. The potential dynamics in the solution by the modified Gouy-Chapman model becomes identical to the Stern model if the main characteristics of Stern layer are attributed.

3-9

Authors: Valentina BULIMAGA, Liliana ZOSIM, Maria PISOVA, Valeriu RUDIC* , Sergiu ŞOVA

Spirulina productivity and iron content in biomass recorded higher values at light intensity 5500 lx, compared to 3500 lx, but phycobiliproteins accumulation in spirulina biomass was more significant at 3500 lx. The content of the accumulated selenium in the phycobiliproteins registered the increased values with the increasing of selenite concentra¬tion, attesting its maximum value at 45 mg/l, at the both light intensity. Selenium content in the phycobiliproteins at 3500 lx was by 1.4-1.5 times higher, compared to that registered at 5500 lx.

16-20

Authors: Angela RUDAKOVA., Valentina BULIMAGA, Maria PISOVA, Veaceslav REVA, Natalia CLIMOVA, Sergiu RUDAKOV, Liliana ZOSIM

A comparative evaluation of papain and trypsin hydrolysates of phycocyanin and Se-phycocyanin after separation by HPLC with reverse phase on the ResourceTMRPC column was performed.The chromatographic profile of peptides from the trypsin and papain hydrolysates of phycocyanin at separation by gel filtration on Sephadex G-50 column was analyzed. UV-VIS spectra of peptides fractions obtained by gel filtration separation of phycocyanin hydrolysates revealed the presence of chromopeptides.

25-28

Authors: Galina DOBROJAN

This article reflects a systematization of the results presented in the literature in the field. The research approaches genus Anabaenopsis by studying history, systematic changes, highlighting the number of species and taxa of algae that are assigned of genre and some specific morphological characteristics.

34-38

Authors: Maria DUCA, Angela PORT, Steliana CLAPCO, Adriana ACCIU

The researches both from the scientific publications and three projects included in public databases: Angiosperm Phylogeny Website, version 13, Parasitic Plant Connection, Parasitic Plant Genome Project have been analysed and some correlation between degree of parasitism and genome evolution of Orobancheae have been highlighted. It has been concluded that number of chromosomes, presence of introns, deletion of some regions of plastid genome, provides useful information for studying of genome evolution mechanisms, specific taxonomic affiliations etc.

44-51

Authors: Maria MAGHER

Fire blight, a bacteriosis of fruit-trees and other rosaceous plants, can be a disastrous disease in orchards and is therefore of great economic importance. Many symptoms of fire blight can be seen during short time after infection. Using special microbiological media this pathogenic bacterium has been isolated from samples of infected apple, quince and apple trees. Inoculation tests confirmed its pathogenicity and virulence to host plants.

58-64

Authors: Алла ДОНОС, Светлана ГАРАЕВА, Анна ЛЕОРДА, Влада ФУРДУЙ, Галина ПОСТОЛАТИ, Галина РЕДКОЗУБОВА

The paper highlights the community-acquired children’s pneumonia and recurrent respiratory infections mechanisms of influence in the exchange of free amino acids serum, and shows the qualitative and quantitative amino acid modifications influence in the morphology and function of tissues and organs.

70-75

Authors: Constanţa MADAN

The research purpose is to study and reveal the structure of sexual offenses against minors in Moldova with obvious psychiatric consequences. 100 cases of sexual offenses against children examined at Clincal Department of Forensic Medical Center and at the Archive of the Psychiatric Hospital from Moldova were studied, during the period of 2010-2015. It was established the impact of the sexual abuse to minors according by some points, using an annex statistics.

83-90

Authors: Dumitru BULAT, Denis BULAT, Ion TODERAŞ, Nina FULGA

Under the intensification of anthropogenic pressure in aquatic ecosystems of Republic of Moldova, most of stenobionte species became threatened with extinction, but some species, potentially high adaptive, contrast, in present demonstrates an evident biological progression, in some hydrobiotops causing a stressed invasive effect. For Dniester River (limits of Republic of Moldova) three-spined stickleback – Gasterosteus aculeatus Linnaeus, 1758 is a good example, after the construction of Novodnestrovsk hydropower (1981) became a multidominate species forming to Soroca city a particularly large local population. In this paper are analyzed bio- ecological peculiarities of three-spined stickleback and are highlighted environmental factors that contributed to taxon invasion in Dniester River (limits of Republic of Moldova).

108-113

Authors: Ho Anh TUAN

Analysis and identification of 42 specimens of family Terapontidae collected from Gianh estuary and Gianh downstream in Quang Binh province in north center region Vietnam. We have classified four species: Terapon jarbua (Forsskål, 1775); Helotes sexlineatus (Quoy & Gaimard, 1825); Rhyncopelates oxyrhynchus (Temminck and Schlegel, 1842); Pelates quadrilineatus (Bloch, 1790). Species Rhyncopelates oxyrhynchus (Temminck and Schlegel, 1842) was first discovered in Gianh estuary and Gianh downstream in Quang Binh province in north center region Vietnam.

121-125

Authors: Viorica GLADCHI, Nelli GOREACEVA, Elena BUNDUCHI

In this paper it is presented the results of the multiannual study of the temporal and spatial variations of the organic substances (O.S.) in the Dniester waters on the portion Naslavcea village - accumulation tank from Dubasari. After peculiarities of the regime formation of the Middle Dniester, are highlighted two portions of the river, which differ in content and type of S.O., as well in the degree of pollution of the Dniester waters.

133-141

Authors: Angela LIS

It was studied direct photolysis in aqueous solutions of the thiourea and cysteine on the model system in order to estimate their role in the processes arising in the natural waters. It has been found that both of them, thiourea and cysteine, have been subjected to direct photolysis at irradiation with different artificial light sources. From the results obtained it was found that oxidation rate of these thiols depends directly proportional to the initial concentration of thiol in the system and the emission spectrum of the lamp. There were determined quantum yields of thiourea and cysteine using chemical actinometer K3[Fe(C2O4)3] · 3H2O. It was found that the quantum yield of thiourea is much greater than unity, which shows that on the destruction of thiourea only at the initial stage occurs photochemical reaction, which is followed by chemical reactions, possibly radical reactions, so thiourea can be the initiator of chemical self-purification reactions in the natural waters.

153-157

Authors: Gheorghi NICULIŢA

The presented data confirm the fact that the human society has become one of the factors forming the environment quality. Urban media differ in the ways they influence upon adjacent natural ecosystems. All of them are oriented so as to achieve an ecologic equilibrium that would combine decent living conditions for people from the one hand and a “healthy” environment from the other hand.

164-169

Authors: Ion TODERAŞ, Aurelian GULEA, Elena ROŞCOV, Olga GARBUZ

This work will focus on the study of groups of metalloorganic substances on aquatic organisms. Toxicological testing method of coordination compounds CMT-28 and CMA-18 is used on Paramecium caudatum Ehrnbg ciliates, at concentrations of 100, 10, 1, 0,1, 0,01 uM/L, compared to the prototype. Bioassay method allows us to develop fast and comparative conclusions, avoiding a high risk of errors. The investigation of metalloorganic compounds was performed by the method of serial dilutions in the liquid nutrient medium (1 g nutrient solution / 1l water). As a reference cultures were used ciliates Paramecium caudatum. Infusorians, as objects of research are very comfortable, they reproduce quickly enough, being the most convenient test-objects for study in the laboratory conditions, because of time factor which plays a decisive role and also is more simple to elucidate the impact of abiotic factors (metalloorganic substances) on the process of asexual reproduction of Paramecium caudatum. The activity of substances was evaluated according to the character of the toxicity of the preparations (LT50 and LC50), while the activity of ciliates was studied according to the character of viability and reproductive parameters (increasing or decreasing of numbers and their reproductive rate). The acute toxicity study (LC50 and LT50) of the compounds was performed according to the methodical recommendations on overall toxicity of pharmaceutical remedies. LC50 (lethal concentration, 50%) – concentration which causes death of 50% of test-organisms in a given period of time. LT50 (lethal time, 50%) – time, during which the used concentration of the test compound causes the death of 50% of the test organisms.

178-184

Authors: Tatiana PALAMARCIUC, Angela SÎRBU, Oleg PALAMARCIUC

The synthesis, crystallographic and magnetic studies of Fe(III) compounds with 4-phenylthiosemicarbazone and 4-phe¬nylthiosemicarbazone 2-acetylpyridine are presented. The spectrophotometric studies in solution were realized for Fe(II) ions with the same ligands. The coordination compounds [Fe(L1)2]BF4•CH3OH (1) and [Fe(L2)2]FeCl4 (2) were obtained from alcoholic solutions. Crystallographic studies reveal that the methylation of sulfur atom influence the coordination set on the Fe(III) ion. Compounds 1 and 2 shows different magnetic properties in function of temperature.

189-194

Authors: Roman RUSNAC, Anna RUSNAC, Olga GARBUZ, Nicanor BARBĂ, Aurelian GULEA

In this paper are described methods of synthesis of six organic compounds, were three of then are unknown. The structure of the compounds was determined by NMR spectroscopy 1H, 13C and IR spectra. Anticancer activity was studied using cervical cancer cells HeLa, where the compound N-[4-({2-[1-(pyridine-2-yl)ethylidene]hidrazincarbotioil}amino)phenyl] acetamide showed the highest inhibition, about 84% at concentration of 100 μM/L.

204-213

Authors: Vasile RUSU

It was developed the criterion for delimiting of characteristics for electric double layer (EDL) close to the basal surface and edge surface of pillared Al-montmorillonite, and the EDL characteristics in the Stern model were determined. Depending on the case, for EDL close to the basal surface or edge surface, the main characteristics of Stern layer can be estimated, i.e. the Stern potential , chemical specific adsorption energy Ø and charge density c.

3-9

Authors: Aurelia CRIVOI, Iurie BACALOV, Elena CHIRIŢA, Ana ILIEŞ, Valentin AŞEVSCHI, Lidia COJOCARI, Iulian PARA, Irina BACALOV

In terms of contemporary civilization, man lives in a spacious sound, continually accompanied by a "cortege" noise and vibration of the most varied intensities and effects having more or less aggressive on the comfort and even on his health. Due to its harmful and its presence in all departments of life, noise pollution is a major problem in all countries where we are witnessing a phenomenon of increased noise levels.

15-20

Authors: Ina DIDILICĂ

In this paper there is analyzed the influence of zinc and selenium on male reproduction, as well as the factors that bring to their infertility. In order to overcome the infertility problems, more studies showed that namely the influence of the process of spermatogenesis via the essential factors, participants in spermo-production could regulate the biological properties' growth of sperm. The sperm formation involves a series of molecular and morphological changes in the male germ cells which are represented by the diploid stem cells, the spermatogonia, whose stock is maintained through mitosis, especially in the initial phases of spermatogenesis. Thus, the nutrition supplementation with the above-mentioned mineral elements improve the function and the reproductive system, as well as other vital systems of the organism.

28-31

Authors: Iulian PARA

Diabetes is a disease metabolic chronic what is due to the primary deficit absolute or relative insulin and is characterized by disturbing the predominant carbohydrate metabolism, reduced tolerance to carbohydrates, the presence of glucose in urine, along with impairment and other metabolisms: Lipid and protein.

43-48

Authors: Нина ФУЛГА, Ион ТОДЕРАШ, Дмитрий БУЛАТ, Денис БУЛАТ, Надежда РАЙЛЯН

In the context of middle Dniester the three-spined stickleback female spawn three portions of eggs. The ovulation of the fourth generation of eggs in the current breeding season does not occur due to the lack of conditions for the completion of trophic substances accumulation. Before sweeping the next generation oocytes the value of gonadosomatic index is significantly decreasing (P≥0.95), which indicates a decrease in the relative weight of the ovaries and in the number of yolk oocytes, forming the second and third generation of the eggs of the given breeding season. The value of absolute fecundity of females significantly increases with the length and weight of the fish's body (P≥0.95), while the value of the relative fertility significantly decreases with increasing of age of producers (P≥0.99).

56-60

Authors: Ala CHERDIVARĂ, Angela RUDAKOV, Andrei ŞUTOV

Among seed storage proteins, the peanut 7S globulin Ara h1 exhibits a highest allergenicity. Multiple antigen determinants (IgE epitopes) have been identified inside the Ara h1 amino acid sequence. The presence of potential IgE epitopes inside structurally equivalent homologous sequence regions of 7S globulins from other plants (mainly from soybeans) was shown. Probable existence of a cross-immunoreactivity between Ara h1 and the subunits of soybean 11S globulin Gly m G5 was demonstrated.

72-75

Authors: Alina BEŞLIU, Elena CHIRIŢA, Agafia USATÎI

The present work provides the results of the research of the influence of the inorganic nanoparticles TiO2 and Fe3O4 with the size of 30 nm, in different concentrations, on the growth process and biochemical composition of biomass stalk of the yeast Rhodotorula gracilis CNMN-Y-30. It has been found that nanoparticles at concentrations of 0.5 to 5.0 mg/L did not significantly alter the reproduction of the cells and the productivity of the yeast. The positive effect is manifested by favorable changes in carbohydrate and protein content, at the cultivation in the presence of the nanoparticles TiO2 and Fe3O4.

80-85

Authors: Elena SAŞCO

The following characters have been studied: the length of embryonic root and stem at early ontogenetic stage, the callus frequency of mature embryos, callus area and biomass of autumn common wheat parent genotypes Niconia, Odeschi 267 and reciprocal hybrids F1, F2, BC derived from reciprocal crossover of the genitors. It has been determined that maternal genitor Niconia was involved in the development of growth characters in optimum conditions (witness). On background with culture filtrate H. avenae the resistant genotype Odeschi 267 manifested as a maternal and also as paternal donor for the root and only maternal – for the stem. The hybrids of reciprocal combinations have demonstrated high specificity in phenotypic manifestation of callus frequency and callus area characters, between indexes being established negative correlations in witness and background choices with FC H. avenae. Genotype Niconia has been attested as an efficient genitor for the heredity of callus frequency, while for the callus area character – parent Odeschi 267. In phenotypic expression of callus genesis maternal effect manifested for characters at hybrids F1, F2, while the paternal form influenced the alternative proportion of additive factors in character expression at retro crossover hybrids. Through cluster analysis (dendrograms, k-means) of the growth index and callus genesis, it has ascertain similarities and repartition differences of reciprocal hybrids, the manifestation of genes that control characters – being influenced by FC H. avenae.

91-94

Authors: Irina STRATULAT, Victor ŞALARU, Sergiu DOBROJAN

In this study is presented the biochemical components of algal biomass Nostoc flagelliforme (Berk et Curt) Elenk. cultivated in laboratory conditions in Z-8 medium. The algal biomass has the following chemical content: Mn – 683,9 mg/kg, Zn – 131,5 mg/kg, Pb – <10 mg/kg, Fe – 1,09 g/kg, Na+ - 2,10 g/kg, K+ – 8,01 g/kg, Mg2+ – 5,40 g/kg, Ca2+ – 7,94 g/kg. The highest amount of amino acids are specific for: glutamic acid – 3,8012 mg/100 mg, aspartic acid – 2,7838 mg/100 mg, leucine – 2,4651 mg/100 mg, alanine – 2,3513 mg/100 mg. According to these results the blue-green alga Nostoc flagelliforme can be considered an important potential source of nutritional supplements.

103-107

Authors: Ludmila CUHARSCAIA

According to the Morld Conservation Union, invasive alien species are the second most significant threat to bio¬diversity, after habitat loss. In this article have been generalized and analyzed last data about specific composition of invasive flora from the Chisinau ecosystem, have been studied and analyzed some eco-biological characters of alien invazive plant species. Was assessed their negative and pozitiv impacts on the urban ecosystem, economy and human health.

122-127

Authors: Tatiana DUDNICENCO

In the results of these studies have been elaborated the ecotourism itinerary of „Rudi-Arionesti” landscape reserve, which are representative and accessible for ecotourists: „Rudi” monastery, „La trei cruci” Roman fortress, „Farfuria tur¬cului” medieval settlement, „Raposatilor” cave, „La santuri” Thracian origin fortress, „Valea Bradului”, „Izvorul Verde”. It have been described the main points for visitation within the selected route. It have been emphasized the ecological problems of „Rudi-Arionesti” landscape reservation which represents one of the basic destinations of ecotourism routes from Donduseni and Soroca districts and have been proposed solutions of its solving.

131-143

Authors: Anastasia PAHOLNIŢCAIA

The paper presents a review of the Schiff bases that contain hydrazinobenzothiazole, 4-aminotriazole and lysine and coordination compounds with these ligands described in the literature. 3d metal coordination compounds with these ligands have different composition, structure and properties. Both ligands and coordination compounds manifest antitumor, antibacterial, and antituberculous activities. Copper(II) coordination compounds with these ligands manifest better anti¬tumor activity than corresponding Schiff bases.

151-152

Authors: Viorica Gladchi

Când Omul ajunge la etapa realizărilor multiaspectuale, de regulă, în momentul atingerii unui jubileu, se opreşte puţin din alergătură, se uită la sine dintr-o parte, examinează toate reuşitele, biruinţele (în luptă cu insuccesele), îşi apreciază culmile atinse pe parcursul vieţii, face o com¬paraţie între „ce este” şi „ce ar fi fost dacă”, neapărat se luminează la faţă şi mulţumeşte Domnului pentru ziua de ieri, cea de azi, dar, mai ales, pentru cea de mâine. Acest Om poate fi asemuit cu Aurelian Gulea, academician, doctor habilitat, profesor universitar, şef al Departamentului Chimie la Facultatea de Chimie şi Tehnologie Chimică a USM.

03-08

Authors: Valentina BULIMAGA, Liliana ZOSIM, Daniela ELENCIUC

The effects of chromium glycinate and lighting regime on spirulina productivity and chromium accumula¬tion in biomass were studied. The stimulatory effect of coordination compound [Cr(Gly)3]Cl (5-30 mg/l) on productivity of spirulina, grown in both lighting regime: continuous and with photoperiod 14/10 hours, as well as chromium accumulation in biomass were established.

15-20

Authors: Angela RUDAKOVA, Andrei SHUTOV, Serghei RUDACOV, Valentina BULIMAGA, Natalia CLIMOVA, Irina KAHOVSKAIA, Maria PISOVA

The elaboration of the procedures of obtaining of bioactive peptides derived from phycocyanin, as a result of it proteolytic hydrolysis presents great interest for researchers in the terms of theirs use as anti-cancer drugs and for other therapeutic properties. It can be assumed that peptides derived from phycocyanin could manifest more pronounced therapeutic effects compared to phycocyanin. Dynamics of phycocyanin proteolisis by papain, as well as mechanism of phycocyanin hydrolysis were studied in the present work.

25-32

Authors: Victor COVALIOV, Valentin BOBEICĂ, Olga COVALIOVA

The combined application of a series of new approaches, including chemical stimulation of methanogenesis, intro¬duction of the compounds precursors of vitamin B12, recirculating of CO2 and adding of H2, have led to the 1,5-2 times enrichment with vitamin B12 of the sludge resulted from anaerob-digestive treatment of post-distillery vinasse. As a result, the quality improvement of vitaminized forage concentrate was reached, at the same time with the intensification of biomethane production, thus enhancing the ecologico-economic efficiency of the vinasse anaerobic treatment. A part of cobalamin is adsorbed on the particles of the forming sludge, whereas the remaining part is subjected to the advanced adsorption treatment on th mineral adsorbent – diatomite. The product formed can be used as a forage additive for cattle and poultry in agriculture.

37-44

Authors: Maria TIMOŞCO, Aliona VELCIU, Victoria BOGDAN

Experimentally in the condition of “in vitro and “in vivo” on laboratory and pattern animals (mice and guinea-pigs) was confirmed the posibility of utilization the microbe preparations CABILAC and ACLOFEM with prophylactic and treatment of the intestinal disorder and diarrhea. In the basis of intensive antagonistic activity at Escherichia, Proteus, Clostridium and Staphylococcus and adhesion capacity advanced, also and results of his tested on animals to be recommended with inculcation in practice.

56-57

Authors: Inga OLARI, Eugeniu DUDNIC, Natalia DUDNIC

All the changes that occur at the level of organs and systems reflected in the composition of the blood, so we chose the study of the action of increased consumption of soybean on the hematological indicators in white laboratory rat.

68-72

Authors: Ştefan RUSU, Dumitru ERHAN, Maria ZAMORNEA, Oleg CHIHAI, Anatol SAVIN, Elena GHERASIM, Galina MELNIC, Vasile BUZA, Mihail PRUTEANU, Tudor ANGHEL

The study on parasitic fauna in wild mammals from natural reservation “Codrii” in Republic of Moldova has revealed the high level of their infestation with fascicles, dicrocelium, strongiles and protista. The red deer (Cervus elaphus Linaeus, 1758) have been infested with Dicrocoelium lanceolatum (12,8%), Fasciola hepatica (9,5%), strongiles larvae (88,0%) and ovocysts of Eimeria spp. (28,2%); the Japanese deer (Cervus nippon Temminsk, 1838) has been infested with D. lanceolatum (14,9%), F. hepatica (10,2%), strongiles larvae (79,8%) and ovocysts of Eimeria spp. (22,4%); roedeer (Capreolus capreolus Linaeus, 1758) – with D. lanceolatum (20,1%), F. hepatica (3,2%), strongiles larvae (89,4%) and ovocysts of Eimeria spp. (38,1%). The wild boar revealed 3,6% level of infestation with Dicrocoelium lanceolatum, larvae of Strongyloides ransomi in 72,3% of cases, Metastrongylus elongatus (87,5%), and Eimeria spp. (46,3%). The jackrabbit revealed 28,2% infestation level with D. lanceolatum and 59,4% level of infestation with Strongyloides papillosus. The high level of parasitic infestation of the wild animals again demonstrates that they play an important role in maintaining the epizootic chain of these diseases and also have crucial role in infesting humans and domestic animals. Therefore it is of paramount importance that parasitic diseases in wild animals are monitored on annual basis that allows to determin their evolution, emergence of new parasitic agents and elaborating new combating measures for such.

81-84

Authors: Sergiu DOBROJAN, Irina STRATULAT, Alina TROFIM, Galina DOBROJAN

This article presents the amino acid content of the blue-green alga Nostoc gelatinosum (Schousb) Elenk. collected from the Drew nutritive medium. From the essential amino group in Nostoc gelatinosum (Schousb) Elenk. biomass, major amounts are contained leucine (2,142 ± 0,06 mg/100 mg), threonine (1,188 ± 0,02 mg/100 mg) and valine (1,085 ± 0,03 mg/100 mg). From nonessential amino acids predominates aspartic acid (4,523 ± 0,11 mg/100 mg) and glutamic acid (2,774 ± 0.07 mg/100 mg). The alga Nostoc gelatinosum (Schousb) Elenk. has a high content of amino acids and can serve as a source for vast areas of application.

88-94

Authors: Alina TROFIM, Victor ŞALARU

In this article are presented structural features of edaphical algal flora of greenhouses from village Chistelnita. Taxonomic structure consists of 53 species and varieties of algae belonging to 31 genera, 22 families, 16 orders and 7 classes, of which the highest diversity have Hormogoniophyceae and Xanthophyceae classes, with 16 and 14 species. Ecological characteristics of algal flora demonstrates the predominance of P ecobiomorphic, which are devoid of heterocyst and spread through the particles of ground or on the surface of it. In soils algal flora were discovered four species belonging to CF ecobiomorphic and they are nitrogen fixing: Anabaena sp., Cylindrospermum licheniforme (Bory.) Kutz., Nostoc linckia (Roth.) Born. et Flah. and Nostoc sp., which according to data from the literature, is promising strains for promoting organic farming.

103-109

Authors: Regina FASOLA

Within the present study was assessed the content of heavy metals in the soils of the Cereşnovăţ river basin, and particularly within the forest ecosystem “Racovăţii de Sud”. The content of heavy metals in the soils of the Cereşnovăţ river basin was determined according to the methods recommended by ICP Forest program after soil types, on fixed layers of 10 cm. Based on the outcomes was observed that the accumulation of heavy metals is dependent and influenced by various factors: human factor, the distance from the pollution source, the terrain, the physical properties of migration of heavy metals and soil type. It was found that low - medium content of heavy metals in the soils of Cereşnovăţ river basin presents favorable ecological conditions to biotic components of the forest ecosystem “Racovăţii de Sud”. Results allow us to conclude that the health of biodiversity and functioning capacity of the ecosystem are not threatened by edaphic factor.

114-118

Authors: Maria SANDU, Galina DRAGALINA

The quantity of water on the planet is estimated at 1,338 million km3 of which only 2,57% is freshwater. But from liquid freshwater, only 3% is expected to be taken for human consumption, which, in fact, is in growing. For example, global water consumption has tripled from 1950 to 1990 and in the period of 1990-1995 years it increased six times. Problems related to water quantity and quality are related to its uneven distribution around the globe, by reducing the amount of potable water, increasing pollution with organic substances, nitrogen compounds, heavy metals, etc. Water quality can be improved only by empowering all citizens in the Republic of Moldova, by motivating them to get involved in all activities to protect waters in the territory. This study comes to argue the importance of examining water quality, listing the causes of pollution, involvement in all activities to prevent and reduce pollution.

125-129

Authors: Valentin CODREANU

As a result of quantitative anatomic study of leaf blade of 5 species of genus Clematis L., is established that anomocytic morphological type of stomatal apparatus (complex) is characteristic for the abaxial and adaxial epidermis. Stomatal density of leaf epidermis of clematises is specific, but varies in different years. The density of leaf stomata characterizes every species and varies, in drought 2007 year, from 119,05/mm2 at species Clematis vitalba to 182,05 stomata/mm2, at species C.tangutica. The greater average thickness of leaf blade and present of stomata in adaxial epidermis of leaf blade evidence more drought resistant species of clematises.

142-155

Authors: Mihail SECU

There were highlighted manganese(III) coordination compounds with Salen type ligands, which exhibit single molecule magnet (SMM) and single chain magnet (SCM) behaviour. It was narrated the principle of assembly bis(salicylidene)isothio¬semicarbazide similar with Salen type ligands, for identify and describe the new methods of rational synthesis of single molecule magnets and single chain magnets, which have as building blocks these manganese(III) complexes.

168-175

Authors: Ion ARSENE

Dinuclear manganese based enzymes engage in processes as diverse as hydrogen peroxide disproportionation and α-amino acidhydrolysis. N,N’-bis(2-pyridylmethyl)-ethylamine dimanganese complexes manifest a high catalytic activity in the hydrogen peroxide decomposition in two stages. In this work the results of DFT calculations on two complexes, [(L)2(Mn(III))2(CH3CO2)2(OH)2]2+ and [(L)2(Mn(IV))2(CH3CO2)2(O)2]2+, involved in steps (I) and (II), different values of the total spin are reported. According to these calculations it results that both systems with high-spin electronic terms have lowest energy. The general energy gain calculated in this processes is -46.56 kcal/mol and this fact confirms the feasibility of this reaction.