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Growth Kinetics of Four Fresh Water Isolated Microalgae for Optimal Biomass and Lipid Production using Response Surface Methodology
Authors: Shakeel Ahmed Adhoni, Shanthanu M. Raikarand C.T. & Shivasharana
Number of views: 377
Microalgae require a wide range of chemical and physical factors for their growth, proliferation, and
differentiation. These factors affect the morphology, physiology and metabolic activities of the organism. Therefore, in the
present investigation, lipid producing microalgae were isolated and identified, abiotic growth factors such as different
autotropic media, pH, nutrients, and media optimization studies were carried out to obtain highest biomass and lipid from
microalgae which have the potential for effective biodiesel production. Growth studies were carried out of the isolated
algal strains, the optimum temperature for all the experiments was kept at 25 ± 1 oC and light intensity of 1.2 ± 0.2 klux
was maintained. Four strains with highest percentage wise Biomass : Lipid ratio were considered for studies, these strains
are Chlorella vulgaris AS-3, Chlorella pyrenoidosa AS-6, Scenedesmus dimorphus AS-13, all 3 strains were isolated from
unkal lake and Scenedesmus quadricauda AS-18 was isolated from Rayanaal lake. Out of the four isolated strains
Chlorella vulgaris (AS-3) showed a significant increase in lipid content by 3 %. The organism was found to grow well in
the optimized BG-11 media with the pH of 6.5 and culture age of 6 weeks and concentration of sodium nitrate, FAC,
potassium bicarbonate and magnesium sulphate of 2, 0.24, 0.3 and 0.8 g/L respectively. The data obtained by the by the
above growth studies can be used for mass culturing of the organism in invivo conditions in open ponds and furthers
studies can be done using different media compositions. Hence, AS-3 was found to be an ideal candidate for biodiesel
production and further characterization of the microalgae was carried out.