A 1.1 Mb duplication CNV on chromosome 17 contributes to skeletal muscle development in Boer goats
Authors: Ying Yuan, Wei-Yi Zhang, Bai-Gao Yang, Dong-Ke Zhou, Lu Xu, Yong-Meng He, Hao-Yuan Zhang, Cheng-Li Liu, Yue-Hui Ma, Ming-Xing Chu, Wen-Guang Zhang, Hui-Jiang Gao, Lin Jiang, Fu-Ping Zhao, Lu- Pei Zhang, Ri-Su Na, Baatarchogt Oyungerel, Yan-Guo Han, Yan Zeng, Shi-Zhi Wang, Huai-Zhi Jiang, Hong- Ping Zhang, Xun-Ping Jiang, Jian-Ning He, Hao Liang, Kaushalendra Kaushalendra, Ya-Wang Sun, Yong- Fu Huang, Yong-Ju Zhao, Zhong-Quan Zhao, Guang-Xin E
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The Boer goat is one of the top meat breeds in modern animal husbandry and has attracted widespread attention for its unique growth performance. However, the genetic basis of muscle development in the Boer goat remains obscure. In this study, we identified specific structural variants in the Boer goat based on genome-wide selection signals and analyzed the basis of the molecular heredity of related candidate genes in muscle development. A total of 9 959 autosomal copy number variations (CNVs) were identified through selection signal analysis in 127 goat genomes. Specifically, we confirmed that the highest signal CNV (HSV) was a chromosomal arrangement containing an approximately 1.11 Mb (CHIR17:60062304–61171840 bp) duplicated fragment inserted in reverse orientation and a 5 362 bp deleted region (CHIR17:60145940–60151302 bp) with overlapping genes (e.g., ARHGAP10, NR3C2, EDNRA, PRMT9, and TMEM184C). The homozygous duplicated HSV genotype (+/+) was found in 96% of Boer goats but was not detected in Eurasian goats and was only detected in 4% of indigenous African goats. The expression network of three candidate genes (ARHGAP10, NR3C2, and EDNRA) regulating dose transcription was constructed by RNA sequencing. Results indicated that these genes were involved in the proliferation and differentiation of skeletal muscle satellite cells (SMSCs) and their overexpression significantly increased the expression of SAA3. The HSV of the Boer goat contributed to superior skeletal muscle growth via the dose effects of overlapping genes.