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Zero order and first order derivative Spectrophotometric determination of Metformin HCL in bulk dosage form
Authors: Rajesh Shukla, Sunil Singh, Jay Ram Patel, Sarita Kare, Reenu Yadav
Number of views: 457
Objective: A simple, accurate, sensitive and precise Zero order and First order Ultraviolet spectrophotometric method has been developed for the determination of Metformine HCL in bulk dosage form. Materials and methods: The solutions of standard and sample were prepared in methanol. In the UV specrophotometric method, the quantitative determination of the drug was carried for zero order at 234nm and first order at 246nm, the linearity range was found to be 2-12 μg/ml and 2-12 μg/ml respectively. Results: The calibration graphs constructed at their wavelength of determination were found to be linear for zero and first derivative spectrophotometric methods. The proposed methods have been extensively validated statistically that included parameters such as linearity, accuracy, precision, LOD, LOQ, recovery and robustness. There was no significant difference between the performance of the proposed method regarding the mean values and standard deviations. Conclusion: The described methods can be readily utilized for analysis of pharmaceutical formulation.