Asian Journal of Chemistry

2143-2146

Authors: Devendra Kumar* and Shiva Sharma

India is the largest producer of fruits as they are important part of their economy. But they are badly affected by insect-pest attack during harvesting. Farmers use a large amount of pesticides to protect them but due to their longer persistence they found in fruits in small quantities. This paper described an analytical methodology for the monitoring of 20 organochlorine pesticides in 5 fruits viz. pineapple (Ananas comosus), apple (Malus pumila), plum (Prunus domestica), papaya (Carica papaya) and mango (Mangifera indica) by using gas liquid chromatography equipped with electron capture detector (GLC-ECD). During the monitoring work it has been found that each fruit was contaminated with pesticides. Pineapple was found contaminated with β-BHC, δ-BHC, heptachlor epoxide, dieldrin, endrin and methoxychlor; apple was found contaminated γ-BHC, δ-BHC, heptachlor epoxide, dieldrin, endrin, endosulfan II and methoxychlor; plum and papaya were found contaminated with γ-BHC, β-BHC, δ-BHC, heptachlor epoxide, 4,4′-DDE, endrin, endosulfan sulfate and methoxychlor; mango was found contaminated with β-BHC, δ-BHC, 4,4′-DDE, endrin, endosulfan sulfate and methoxychlor. However, the detected concentration of pesticides was below the MRL values but their long term use can cause serious health problems.

2152-2156

Authors: V. Lekha and P. Pushpaletha

This study reports the indoor ozone (O3) pollution in the rural location of Kannur, a tropical site in India, during March 2018 and the influence of their most important determinants. Measurements of indoor O3, NO, NO2 and NOx were carried out in a residential building for a period of one week. Measurements were taken from the kitchen, as it had been identified as the most polluted spot. The background concentrations of O3, NO, NO2 and NOx were also monitored. Indoor O3 originates mainly from the outdoor environment and is also produced from indoor sources. The results show that there is a higher level of indoor O3 during cooking hours. The study indicated that cooking activities in the kitchen had a strong impact on the indoor O3 level. Ozone is an important gaseous pollutant which may lead to severe health problems such as decreased lung function and respiratory symptoms.

2165-2172

Authors: U.G. Ghoshir, S.R. Kande, G.G. Muley and A.B. Gambhire

A series of solid base fly ash hybrid materials were synthesized by doping alkali, alkaline earth metals with nitrogen, separately using co-precipitation process, combined with surfactant incorporating method. The catalysts were characterized by X-ray diffraction, scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy and photoluminescence spectroscopy. Results revealed that the co-doped hybrid materials are highly stable with particle size in the range of 40-60 nm. The surface basicity of fly ash was upraised by increased hydroxyl content by doping with alkali, alkaline earth metals with nitrogen. The basicity of hybrid material was measured by liquid phase, solvent free, single step condensation of 4-chlorobenzaldehyde and acetophenone giving higher conversion rate and selectivity of desired product chalcone. This conversion showed that the fly ash based hybrid material has sufficient basic site, responsible for the catalytic activity

2181-2184

Authors: Abdulrahman I. Alharthi

The effect of calcination on the performance of tungstophosphoric acid for the product of Knoevenagel condensation was investigated. Substituted aldehydes and dimedone has been used in the presence of calcined tungstophosphoric acid as a heterogeneous catalyst using grinding method at room temperature. The results of reactions revealed that calcined tungstophosphoric acid has superior catalytic activity comparing to non-calcined catalyst in terms of yield and reaction time. Maximum yield of model compound was achieved by using 10 mol% of calcined catalyst in a reaction time that does not exceed 10 min, whereas the yield at same amount of non-calcined catalyst was 86 % in a reaction time of 35 min.

2191-2196

Authors: S. Rathinamanivannan, K. Megha, Raja Chinnamanayakar, Ashok Kumar and M.R. Ezhilarasi

The new series of 1-(4,5-dihydro-5-phenyl-3-diphenylpyrazol-1-yl)butan-1-one derivatives were synthesized by cyclization method using biphenyl chalcone with n-butyric acid and hydrazine hydrate. The synthesized 1-(4,5-dihydro-5-phenyl-3-diphenylpyrazol-1-yl)butan-1-one derivatives chemical structures were confirmed from spectral data such as FT-IR, 1H and 13C NMR. 2-Pyrazoline derivatives were docked with bacterial (1UAG) and breast cancer (1OQA) protein. Based on high binding affinity score, the best compound was subjected to in vitro anticancer activity by MTT assay. Also, antimicrobial activity were studied for synthesized 2-pyrazoline derivatives

2201-2210

Authors: Sadaf Zaidi, Devdutt Chaturvedi, Mridula Saxena and Richa Srivastava

A series of dithiocarbamates of ω-substituted (2-naphthyloxy) alkanes was developed through condensation of 2-(2-chloro-alkoxy)-naphthalene to various kinds of aliphatic, aromatic, alicyclic, heterocyclic primary and secondary amines employing benzyl trimethyl ammonium hydroxide in catalytic quantity (Triton-B/CS2 system) afforded desired products in high yields (82-98 %). The complete series of synthesized compounds (4-48) were evaluated for antimicrobial activity through microdilution method using various bacterial and fungal strains. The antifungal and antibacterial values were estimated as MIC values. Fluconazole and ciprofloxacin [16 to 0.03 μg/mL] were used as the standard antifungal and antibacterial drug, respectively. Out of series of evaluated compounds, some of these compounds such as compounds 28, 29, 30, 31, 32, 33 have displayed maximum potency which is comparable to standard drugs.

2215-2221

Authors: P. Suresh Kumar, G.V. Krishna Mohan and A. Naga Babu

A novel and simultaneous stability indicating RP-HPLC method has been developed for quantitative analysis of bumetanide in fixed dose pharmaceutical formulations. Bumetanide and its degradation products are well separated by the Discovery C18, 250 × 4.6 mm, 5 μm column as a stationary phase and (50:50 v/v) of 0.1 % o-phthalaldehyde and acetonitrile as a mobile phase. All the compounds are monitored using photodiode array detector at 254 nm with an isocratic method and the flow rate of 1.0 mL/min was maintained. Validation of method was performed as per International Council for Harmonization (ICH) guidelines and the parameters namely; precision, accuracy, specificity, stability, robustness, linearity, limit of quantitation (LOQ) and limit of detection (LOD) were evaluated. The linearity of the proposed method was found to be 0.315-1.875 μg/mL for bumetanide and its impurities. The developed method is more economical and suitable for laboratory use because of solvent consumption is very less. Hence, the developed method can be used for the determination of bumetanide and its impurities in drug product stability studies and pharmaceutical formulations

2229-2232

Authors: M. Alexander, R.A. Kalaivani, V. Sriraman and U. Senthilkumar

Fabricated titanate nanotubes (f-TNT) are prepared by chemical deposition method using linen fiber. The f-TNTs is fabricated by TiO2 nanoparticles, the each TiO2 nanoparticles diameter has 80-100 nm in range. The Pd0 nanoparticle was coated on the surface of the f-TNT by chemical reduction method, using NaBH4 as reducing agent. The prepared Pd/f-TNTs characterized by FT-IR, FE-SEM and HRTEM, the result shows that the f-TNT diameter is 1500 nm and Pd nanoparticles range in 20-50 nm. The Pd/f-TNT nanocomposite modified glassy carbon (GC) electrode shows quasi irreversible redox behaviours in cyclic voltammeter. Those Pd/f-TNTs modified electrode is utilized for electrochemical polymerization of o-phenylenediamine in acid medium

2240-2244

Authors: Vihar Patel, Akshit A. Patel, Bharat Dixit and Ritu Dixit

Removal of dyes colour and Cu(II) metal ion from aqueous solution using acetone-formaldehyde-salicyclic acid (AFSA) resin has been carried out. The results revealed that AFSA resin acts as a cheap substitute to commercial adsorbent like activated carbons. The effects of pH, adsorbent dose, contact time, and initial dye and Cu(II) metal ion concentration on the adsorption efficiencies were investigated. Isotherm studies were conducted using Langmuir and Freundlich models, and thermodynamic studies were also performed. Adsorption of dyes was found to obey the Langmuir isotherm model and have endothermic process. The maximum adsorption capacities calculated from the Langmuir isotherm were found to be 88.18 and 2.9 mg/g for methyl orange dyes.

2249-2256

Authors: Ntaote David Shooto and Eliazer Bobby Naidoo

The present work reports the biosorption studies of metal ions viz., Pb(II) and Cu(II) from aqueous solution onto paw-paw seeds (PPS), acid treated paw-paw seeds (ATPPS) and base treated paw-paw seeds (BTPPS) adsorbents by batch method. A series of tests were carried to evaluate the effect of the system parameters, i.e. adsorbent dosage, initial concentration, temperature and contact time. The obtained kinetic data showed that the pseudo first order model best fitted the biosorption of both metal ions with (r2) values of 0.9918, 0.9674 and 0.9463 for Cu(II) onto PPS, ATPPS and BTPPS, respectively and for Pb(II) 0.8513, 0.8686 and 0.9434 onto PPS, ATPPS and BTPPS respectively. Estimated surface adsorption of intra-particle diffusion estimated that surface sorption dominated. Thermodynamic parameter (ΔGo) gave negative values indicating that the biosoption processes were spontaneous and feasible. ΔHo gave positive and negative values indicating that some biosorption processes were endothermic and some exothermic. Isotherm data indicated that Langmuir best described the biosorption. The reusability of PPS, ATPPS and BTPPS adsorbents was evaluated up to five cycles without showing significant drop in sorption efficiency

2261-2268

Authors: R. Suganthi and S. Jhancy Mary

Fabrication of substituted polyaniline nanocomposites with CuO results in hybrid materials with enhanced synergistic properties. Hence poly(2-chloroaniline)-composite-CuO, poly(2-chloroaniline)-composite-CuO/SDS, poly(2-methoxyaniline)-composite-CuO/SDS and poly(2-methylaniline)-composite-CuO/SDS nanocomposites were prepared chemically by in situ oxidative polymerization method. Characterization by a number of techniques such as FTIR, NMR and UV-visible spectroscopic methods, XRD and TEM are presented. The substituted polymers exhibited an appreciable interaction with the CuO (5 wt.%) nano fillers. Integral Procedural Decomposition Temperature (IPDT) and Oxidative Index(OI) calculations were done to establish the stability to heat. Thermal stability of the materials follows the trend p2ClAni-CuO-SDS > p2ClAni-CuO> p2MeAni-CuO-SDS> p2OMeAni-CuO-SDS. The electrical conductivities of poly(2-chloroaniline)-composite-CuO and poly(2-chloroaniline)-composite-CuO/SDS measured are 1.46 × 10-7 and 1.59 × 10-7 S cm-1, respectively and the presence of anionic surfactant does not change the electrical conductivity behaviour. The poly(2-methoxyaniline)/CuO-SDS and poly(2-methylaniline)/CuO-SDS exhibit an electrical conductivity of 1.68 × 10-6 and 1.24 × 10-6 S cm-1 respectively. The dielectric constant decreased with increase in frequency in the low frequency region due to electrical relaxation process. At low frequency there was a strong frequency dispersion of permittivity and above 2.5 Hz a frequency independent behavior was noted

2275-2283

Authors: Suresh Kumar Palacharla and G.V. Krishna Mohan

A single HPLC method for the determination of four active ingredients viz. aspirin, rosuvastatin, ezetimibe and clopidogrel in less run time is developed. HPLC method was developed and validated. X-terra C18 100 × 4.6 mm, 3.5 μ HPLC column, KH2PO4 buffer (mobile phase A) and acetonitrile (mobile phase B) were used. 20 μ injection volume, 1.0 mL/min flow rate, 230 nm and ambient column oven temperature were applied for separation. Gradient program: at 0 min mobile phase-B 13 %, at 4 min 13 %, at 8 min 44 %, 14 min 57 %, 17 min 57 %, 20 min 13 % and 25 min 13 %. Method validation was performed as per ICH guidance with precision, linearity, specificity, accuracy, ruggedness and robustness. Validation results were satisfactory and this method can be applied for regular drug product manufacturing

2287-2290

Authors: S. Pitchuanchom, M. Nontakitticharoen and H. Thaisuchat

The aim of this study is to report the development of Escherichia coli O157:H7 template for structure-based drug design. This template was validated by redocking with crystal ligand I. The results showed a good matching of docked and the crystallographic binding orientations with root mean square deviation (RMSD) less than 2.0 Å. Moreover, the developed template was applied to predict binding mode of 19 known E. coli inhibitors and 40 natural products. The results showed that the binding energy of almost E. coli inhibitors was related to their biological activity. The use of developed E. coli O157:H7 template in automated docking could speed up the process of novel drug discovery by allowing designed inhibitors to be tested computationally before the compounds are synthesized.

2298-2302

Authors: Dinh Nhat Do, Ngoc Tu Pham, Tri Duc Lam, Long Giang Bach, Duy Chinh Nguyen, Quoc Toan Tran4 and Le Thi Hong Nhan

Present study involves to the pilot scale hydrodistillation process for production of essential oil of orange peels. The production of orange peels essential oil and the effect of several factors such as time, temperature and the ratio of solid/solvent on yield and quality were studied. In this work, we used fresh orange peels of C. sinensis (orange). The quantitative and qualitative analyses of the essential oils of orange peels were performed by GC/MS and sensory analysis. The maximum yield of orange peels essential oil was 1.2384 % (fresh matter) at distillation conditions (ground fresh material, the material-water ratio of 1:2, time of 105 min from the first drop, the temperature of 132 ºC). Kinetic studies of hydrodistillation process showed that the extraction of orange peels essential oil follows first order kinetic (R2 > 0.95). Limonene is the dominant component of the orange peels essential oil that was identified by chromatography-mass spectrometry (94.22 %). The results of test indicated the orange peels essential oil has good qualities which were suitable to the National Standards of Vietnam.

2306-2310

Authors: S. Vidhya, A. Leema Rose and F. Janeeta Priya

Adapting eco-friendly methodologies is the need of the hour in the every field of research. In the present work an investigation on Gomphrena serrata leaves was evaluated as a corrosion inhibitor for the mild steel in HCl medium and as a reductant. The G. serrata as an efficient inhibitor it obeys Langmuir adsorption isotherm and served as anodic type inhibitor. The impedance study confirms the formation of thin film on the metal surface through its Cdl and Rct values. The copper nanoparticles and their derivatives have been used as a medicine to prevent infection, leg ulcers etc. Gomphrena serrata leaves have good medicinal values, to enhance its medicinal properties it was mediated for the synthesis of copper nanoparticles. The CuNPs formation was confirmed by SPR bands at 345 nm by microwave method and at 341 nm by thermostat method. The functional groups present in the G. serrata also present in the FTIR spectra of CuNPs and it confirms the G. serrata was used as a reducing as well as a capping agent

2319-2322

Authors: Z. Alfian, H. Marpaung, M. Taufik, S. Lenny, Andriayani and S.J. Samosir

Essential oils are volatile oils with different compositions according to their source of production and comprise a mixture of substances having different chemical and physical properties. One of the plants containing essential oil is Eucalyptus grandis grown at PT Toba Pulp Lestari (Indonesian company). The purpose of this work is to know the chemical contents and physical properties of essential oil from Eucalyptus grandis as well as to utilize unused leaves at PT. Toba Pulp Lestari to serve as an essential oil. The essential oil of Eucalyptus grandis leaves was extracted using hydrodistillation method, then essential oil is analyzed by GC-MS. The essential oil of Eucalyptus grandis leaves was obtained 0.4 %. The physical properties test with determination of density and refractive index of essential oil respectively were obtained at 0.9143 and 1.4653 nD at 29.4 ºC. The results of GC-MS analysis of essential oil showed 12 components viz. α-pinene (45.21 %), camphene (1.38 %), β-pinene (1.11 %), camphogen (0.74 %), 1,8-cineole (36.55 %), α-campholene aldehyde (0.73 % ), pinocarvone (0.83 %), α-terpineol (8.87 %), β-caryophyllene (1.72 %), spathulenol (0.84 %), elemol (0.85 % ) and 1-nonadecene (1.17 %).

2327-2330

Authors: Afrizal, Arif Rahman, Nurul Hidayati and S. Iwan

Monomer cholesteryl acrylate was doped by indium tin oxide (ITO) which succeeded in becoming composites in photopolymerization process using UV curing method having various polymerization time (15, 20, 25, 30 and 35 min) at 60-70 °C. The goal of this study is to know the effect of polymerization time to characterize the conductivity properties of composite cholesteryl acrylate-ITO. All the spectra confirmed the peaks at 600-550 cm-1 which attributed the doping of ITO in the composite cholesteryl acrylate-ITO. The XRD patterns of polymer cholesteryl acrylate-ITO has nematic chiral phase at 2θ = 15-20°. Some of the peaks are appeared at 15.2734° and 16.7266°, while other peaks of indium tin oxide appeared at 30°, 50° and 60°. The surface morphology of polymer cholesteryl acrylate-ITO showed the existence of sphere. The value conductivity of polymer cholesteryl acrylate-ITO is found to be 1.8390 × 10-10 S/m.

2337-2340

Authors: R. Ganesamoorthy and P. Sakthivel

Ten donor-acceptor based (D-A) cyano-stilbene derivatives were synthesized and their structure was confirmed by 1H NMR, GC-MS, FT-IR. The absorption was calculated by UV-visible spectrometer. From the UV-Vis study, stilbenes are having broad and strong absorption in the UV-visible region. The strong and broad absorption in the UV-visible region is the desired property for the high power conversion efficiency organic photovoltaic cells

2345-2350

Authors: Nguyen Thi Van Linh, Vuong Thi Ngoc Mai, Tran Thi Yen Nhi and Tri Duc Lam

This study aims to investigate the effect of xanthan gum and carboxymethylcellulose on physical and chemical qualities of green asparagus juice. We adopted the surface-response method and the CCD experiment design with respect to three response variables including stability, viscosity and colour of the product. It was revealed that both xanthan gum and carboxymethylcellulose concentrations are both positively correlated with viscosity and stability of the product. In addition, the coefficient of the interaction of xanthan gum and carboxymethylcellulose was not significant (p < 0.05). In sensory evaluation, both carboxymethylcellulose and xanthan gum were found to be influential on product state. However, hydrocolloid concentration effects were not profound on perceived product colour and odour

2357-2360

Authors: Jagvir Singh, Anuradha, Pradeep Kumar, Mordhwaj and Ravi Kumar

Co(II), Ni(II) and Cu(II) metal complexes of a novel Schiff base ligand (SBL) derived from condensation of 2-(4-fluorophenyl)prop-2-enaln and ethane-1,2-diamine were synthesized. All the complexes were characterized by elemental analyses, IR, UV-visible spectroscopy, magnetic susceptibility and conductance measurements and 1H NMR. From the elemental analysis data, 1:1 [M]:[ligand] metal chloride complexes are formed having the general composition [M(SBL)Cl2] and [Cu(SBL)] Cl2, [where M = Co(II), Ni(II) and SBL= {bis-2-(4-fluorophenyl)prop-2-enaln}ethane-1,2-diamine]. The result showed that the ligand is coordinated to the metal ions in a neutral tetradentate manner with ON donor sites and the nature of metal-ligand bonding can range from covalent to ionic. Antimicrobial activities of the newly synthesized chemical compounds were evaluated against Staphylococcus aureus (ATCC 25923), Staphylococcus aureus (ATCC 3160), Cabdida albicans (ATCC 227) and Staphylococcus cereviscae (ATCC 361) species and found that metal complexes exhibited more antimicrobial properties so they are more potential and significant than the ligand

2367-2369

Authors: Refdinal Nawfa , Adi Setyo Purnomo, and Herdayanto Sulistyo Putro

Penicillin-G antibiotic was used as the basic ingredient of making antibiotic type β-lactam such as tetracycline, amoxicillin, ampicillin and other antibiotics. Penicillin-G was splited into 6-amino penicillanic acid as the source of β-lactam. The biosynthetic pathway for the formation of penicillin-G in Penicillium chrysogenum cell through the formation of intermediates was carried out in the form of amino acids such as α-aminoadipate, L-cysteine, L-valine which are formed from glucose (food ingredients).The formation of 6-amino penicillanic acid is an amino acid combination of L-cysteine and L-valine, a step part of the formation of antibiotic penicillin-G in P. chrysogenum cells, thus, it is obvious that there are enzymes involved in its formation. The objective of this study was to examine the use of enzymes present in P. chrysogenum cells to produce penicillin-G and 6-amino penicillanic acid using the intermediate compounds α-aminoadipate, L-cysteine, L-valine and phenylacetic acid assisted by NAFA® coenzymes in P. chrysogenum cells which is more permeable. The research method started from producing biomass of P. chrysogenum cells that demonstrated penicillin-producing antibiotic capability, as the source of the enzyme, followed by addition of permeability treatment of P. chrysogenum cell membrane to get immobile of enzyme by its own cell therefore it can be used more than once. After that the enzyme activity was proven by adding α-aminoadipate, L-cysteine, L-valine, phenylacetic acid and NAFA® coenzyme for the formation of penicillin-G, whereas the addition of L-cystein, L-valine and NAFA® coenzyme were aimed to form 6-amino penicillanic acid. The results showed that P. chrysogenum is able to produce antibiotics with stationary early phase on day 6. The best increased permeability of P. chrysogenum cell membranes was obtained using a 1:4 of toluene:ethanol ratio mixture with the highest antibiotic concentration (130.06 mg/L) after testing for the enzymatic formation of antibacterial penicillin-G.

2375-2382

Authors: Venkatesh Desappan and Jaisankar Viswanathan

In this work, the modified S-diol were prepared from sesame oil to produce polyol. The thermal responsive vegetable oil-based polyurethane was prepared by polycondensation method. The different diisocyanate react with jatropha-sesame oil polyol to produce higher density with molecular weight of polyurethanes structure and functionality were characterized by Fourier transform spectroscopy, proton nuclear resonance spectroscopy and gel permeation chromatography. The thermal analysis was carried with TG analysis. The kinetic and thermodynamic parameters was calculated by TGA decomposition temperature and compared with PU-1, PU-2 and PU-3. Furthermore, the corresponding decomposition temperature of polyurethanes activation energy were calculated by Coats-Redfern, Sharp-Wentworth and Freeman-Carols methods, and it is investigated with lifetime of polyurethanes. Depending on the physically observed NH and C=O linkages of polyurethane matrix, which enhancing the hard and soft segment to improve their chain mobility. The success of the investigated polyurethanes, the corresponding estimated enthalpy, entropy and pre-exponential factors are much closer with toluene and phenyl based polyurethane matrix

2389-2393

Authors: Sravanthi Siliveri, Harinadha Babu Vamaraju and Shivaraj

In the present work, novel pyrazole fused dihydrofurans synthesized via a chronological addition of N-chloro succinimide and base piperidine to pyrano[3,2-c]pyrazole carbonitrile derivatives in methanol medium. Oxidative difunctionalization was done with the reagent N-chloro succinimide by the addition of both chlorine and alkoxy groups crosswise the chromene double bond. The addition of base results in the construction of dihydrofuran derivatives by ring contraction. The structures of newly synthesized compounds were characterized on the basis of physical and spectral data. Synthesized compounds were evaluated for antibacterial and anti-inflammatory activities. All the compounds exhibited significant antibacterial activity against all the four strains of bacteria and their MICs ranged between 1.56 and 12.55 μg/mL. In anti-inflammatory screening, among all the tested compounds, compounds 7, 8, 9, 11, 12, 13, 14, 16, 17 and 18 exhibited significant protection against the edema formation at a concentration of 100 mg/kg

2397-2402

Authors: Harpreet Kaur and Abhinav Sharma

In the present work, waste materials such as pomegranate and orange peels were taken to synthesize nanoparticles by biological method. The method did not involve the use of toxic reducing and capping agents. It uses only the environmentally benign biomolecules for reduction of AgNO3 and subsequent stabilization of silver nanoparticles. The size of pomegranate and orange silver nanoparticles varied from 8-25 nm with nearly spherical morphology as analyzed by XRD and TEM studies. The synthesized nanoparticles were studied for their dye degrading property. Different dyes such as crystal violet, methylene blue, malachite green and Congo red were taken for the study. The results indicated the presence of two diverse mechanism of dye removal. Crystal violet, methylene blue, malachite green was removed by adsorption onto both the nanoparticles, whereas Congo red was reduced by NaBH4 in the presence of catalytic pomegranate silver nanoparticles. Moreover, pomegranate silver nanoparticles have exhibited potent antitubercular activity with MIC value of 6.25 μg/mL and orange silver nanoparticles are moderately active with MIC value of 25 μg/mL. It could be concluded that the pomegranate silver nanoparticles would a promising candidate for drug development and catalysis.

2410-2412

Authors: K. Kavitha Priya and V. Anuradha

From the flowers of Leucas cephalotes, pyridine-3-carboxylic acid ester was isolated. Its structure was established as 2′,5′-dihydroxy-3′-pyridyl-(2,5-dihydroxy)pyridinyl-3-carboxylate on the basis of spectroscopic analysis. To out best of knowledge, this is the first report from Leucas cephalotes in particular and Labiatae family in general.